B0BRB3 (ISPH_ACTPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	APJL_1546

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000098929

Sequences

	Sequence		Length	Mass (Da)
	B0BRB3 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: FFF5B0E5C050478A	FASTA	314	34,093
	10 20 30 40 50 60 MNILLANPRG FCAGVDRAIS IVELALEIHG APIYVRHEVV HNRFVVDGLK AKGAVFVEEL 70 80 90 100 110 120 DEVPDGAIVI FSAHGVSQEV RQEAKRRDLK VFDATCPLVT KVHMQVARAS KKGTKAILIG 130 140 150 160 170 180 HEGHPEVIGT MGQYDNQDAG IFLVESVEDI AKLPVSSQDD LTFMTQTTLS IDDTSDVIEA 190 200 210 220 230 240 LKEKYPAIQG PRKNDICYAT TNRQQAVREL AKQSQLVLVV GSKNSSNSNR LAELASRMGV 250 260 270 280 290 300 PSKLIDGPQD IDPSWLDGVE TIGITAGASA PEVLVQSVVE HLKTLGVTGV SNLEGCEENM 310 VFEVPKELRI HEVK

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JL03.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000687 Genomic DNA. Translation: ABY70098.1.
RefSeq	YP_001652542.1. NC_010278.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B0BRB3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5851054.
GenomeReviews	Gene locus APJL_1546 in contig CP000687_GR.
KEGG	apj:APJL_1546.
PATRIC	20752387. VBIActPle136345_1539.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335228.
OMA	AMAPECD.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	APLE434271:APJL_1546-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_ACTPJ

Accession

Primary (citable) accession number: B0BRB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 26, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program