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B0BR28 (NAPA_ACTPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:APJL_1461
OrganismActinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]
Taxonomic identifier434271 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Protein attributes

Sequence length827 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232Tat-type signal Potential
Chain33 – 827795Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000186349

Regions

Domain37 – 93574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding441Iron-sulfur (4Fe-4S) By similarity
Metal binding471Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding791Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B0BR28 [UniParc].

Last modified February 26, 2008. Version 1.
Checksum: BE020AD5642AF456

FASTA82793,572
        10         20         30         40         50         60 
MELNRRDFMK ANAAMAAAAA AGMTIPVKNV YAADDGIRWD KAPCRFCGTG CSVLVGTKDG 

        70         80         90        100        110        120 
RVVATQGDPD AEVNRGLNCI KGYFLSKIMY GADRVQTPLL RMKDGKFHKE GDFTPVSWDQ 

       130        140        150        160        170        180 
AFTIMAEKVK DILKKKEPNS VGMFSSGQTT IFEGYAKVKL WKGGLRSNTI DPNARHCMAS 

       190        200        210        220        230        240 
AAVAFMRTFG MDEPMGCYND IEKTDAFVLW GSNMAEMHPI LWSRISDRRL SDDKVKVVVM 

       250        260        270        280        290        300 
STFEHRSFEL ADTPIIFKPH SDLAILNYIA NYIIQNDKVN WDFVNKHTKF KRGETDIGYG 

       310        320        330        340        350        360 
LRPDHPRQKA AKNAKTAGKM YDSDFEEFKK IVEPYTLEKA HEISGVPKDQ LETLAKMYAD 

       370        380        390        400        410        420 
PKQTLVSFWT MGFNQHVRGV WVNHMVYNVH LLTGKISTPG CGPFSLTGQP SACGTAREVG 

       430        440        450        460        470        480 
TFVHRLPADM VVTNPKHVEI VEKAWKLPKG TIPTVPGYPA VMQSRMLKDG KLNFLWQLCT 

       490        500        510        520        530        540 
NNMQGGPNIN EEIFPGWRNP ENFIVVSDPY PSVSAVAADL ILPTCMWVEK EGAYGNAERR 

       550        560        570        580        590        600 
TQFWRQQVKG PGESKSDLWQ IVEFSKYFKT DEVWDEALLA QMPEYRGKTL YEVLYKNGQV 

       610        620        630        640        650        660 
DKFDTPTNIP GYINDEAEHF GYYLQKGLFE EYAAFGRGHG HDLADFDTYH QVRGLRWPVV 

       670        680        690        700        710        720 
DGKETLWRYR EGFDPYVKAG EGVSFYGYPD KKAIILGVPY EEPAESPDEE YDLWLCTGRV 

       730        740        750        760        770        780 
LEHWHTGTMT RRVPELHRSF PNNLCWMHPD DAKARGLRHG DKVKLITRRG EIITHLDTRG 

       790        800        810        820 
RNKCPKGLIY TTFFDAGQLA NKLTLDATDP ISGETDYKKC AVKVVKA 

« Hide

References

[1]"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China."
Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. expand/collapse author list , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H.
PLoS ONE 3:E1450-E1450(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JL03.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000687 Genomic DNA. Translation: ABY70013.1.
RefSeqYP_001652457.1. NC_010278.1.

3D structure databases

ProteinModelPortalB0BR28.
SMRB0BR28. Positions 35-826.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING434271.APJL_1461.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY70013; ABY70013; APJL_1461.
GeneID5850761.
KEGGapj:APJL_1461.
PATRIC20752207. VBIActPle136345_1454.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAFTTDEMW.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycAPLE434271:GIX7-1480-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_ACTPJ
AccessionPrimary (citable) accession number: B0BR28
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 26, 2008
Last modified: February 19, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families