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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Actinobacillus pleuropneumoniae serotype 3 (strain JL03)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi47Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi51Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi79Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei81Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei148Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei173Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei177Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei371Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei375Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei481Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei530Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei557Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei793Substrate; via amide nitrogenUniRule annotation1
Binding sitei801Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei818Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:APJL_1461
OrganismiActinobacillus pleuropneumoniae serotype 3 (strain JL03)
Taxonomic identifieri434271 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
Proteomesi
  • UP000008547 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Tat-type signalUniRule annotationAdd BLAST32
ChainiPRO_100018634933 – 827Periplasmic nitrate reductaseUniRule annotationAdd BLAST795

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB0BR28.
SMRiB0BR28.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 934Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 217Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni241 – 245Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni507 – 508Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni717 – 726Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000031441.
KOiK02567.
OMAiTTDEVWP.
OrthoDBiPOG091H060P.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct_NapA. 1 hit.
InterProiView protein in InterPro
IPR009010. Asp_de-COase-like_dom_sf.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
PANTHERiPTHR11615:SF123. PTHR11615:SF123. 1 hit.
PfamiView protein in Pfam
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
SMARTiView protein in SMART
SM00926. Molybdop_Fe4S4. 1 hit.
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiView protein in PROSITE
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0BR28-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELNRRDFMK ANAAMAAAAA AGMTIPVKNV YAADDGIRWD KAPCRFCGTG
60 70 80 90 100
CSVLVGTKDG RVVATQGDPD AEVNRGLNCI KGYFLSKIMY GADRVQTPLL
110 120 130 140 150
RMKDGKFHKE GDFTPVSWDQ AFTIMAEKVK DILKKKEPNS VGMFSSGQTT
160 170 180 190 200
IFEGYAKVKL WKGGLRSNTI DPNARHCMAS AAVAFMRTFG MDEPMGCYND
210 220 230 240 250
IEKTDAFVLW GSNMAEMHPI LWSRISDRRL SDDKVKVVVM STFEHRSFEL
260 270 280 290 300
ADTPIIFKPH SDLAILNYIA NYIIQNDKVN WDFVNKHTKF KRGETDIGYG
310 320 330 340 350
LRPDHPRQKA AKNAKTAGKM YDSDFEEFKK IVEPYTLEKA HEISGVPKDQ
360 370 380 390 400
LETLAKMYAD PKQTLVSFWT MGFNQHVRGV WVNHMVYNVH LLTGKISTPG
410 420 430 440 450
CGPFSLTGQP SACGTAREVG TFVHRLPADM VVTNPKHVEI VEKAWKLPKG
460 470 480 490 500
TIPTVPGYPA VMQSRMLKDG KLNFLWQLCT NNMQGGPNIN EEIFPGWRNP
510 520 530 540 550
ENFIVVSDPY PSVSAVAADL ILPTCMWVEK EGAYGNAERR TQFWRQQVKG
560 570 580 590 600
PGESKSDLWQ IVEFSKYFKT DEVWDEALLA QMPEYRGKTL YEVLYKNGQV
610 620 630 640 650
DKFDTPTNIP GYINDEAEHF GYYLQKGLFE EYAAFGRGHG HDLADFDTYH
660 670 680 690 700
QVRGLRWPVV DGKETLWRYR EGFDPYVKAG EGVSFYGYPD KKAIILGVPY
710 720 730 740 750
EEPAESPDEE YDLWLCTGRV LEHWHTGTMT RRVPELHRSF PNNLCWMHPD
760 770 780 790 800
DAKARGLRHG DKVKLITRRG EIITHLDTRG RNKCPKGLIY TTFFDAGQLA
810 820
NKLTLDATDP ISGETDYKKC AVKVVKA
Length:827
Mass (Da):93,572
Last modified:February 26, 2008 - v1
Checksum:iBE020AD5642AF456
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000687 Genomic DNA. Translation: ABY70013.1.
RefSeqiWP_005619821.1. NC_010278.1.

Genome annotation databases

EnsemblBacteriaiABY70013; ABY70013; APJL_1461.
GeneIDi4850345.
KEGGiapj:APJL_1461.

Similar proteinsi

Entry informationi

Entry nameiNAPA_ACTPJ
AccessioniPrimary (citable) accession number: B0BR28
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 26, 2008
Last modified: January 31, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families