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B0BR28

- NAPA_ACTPJ

UniProt

B0BR28 - NAPA_ACTPJ

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Actinobacillus pleuropneumoniae serotype 3 (strain JL03)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi79 – 791Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciAPLE434271:GIX7-1480-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:APJL_1461
OrganismiActinobacillus pleuropneumoniae serotype 3 (strain JL03)
Taxonomic identifieri434271 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
ProteomesiUP000008547: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
BLAST
Chaini33 – 827795Periplasmic nitrate reductasePRO_1000186349Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi434271.APJL_1461.

Structurei

3D structure databases

ProteinModelPortaliB0BR28.
SMRiB0BR28. Positions 35-826.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 93574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiAGKMHDS.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0BR28-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELNRRDFMK ANAAMAAAAA AGMTIPVKNV YAADDGIRWD KAPCRFCGTG
60 70 80 90 100
CSVLVGTKDG RVVATQGDPD AEVNRGLNCI KGYFLSKIMY GADRVQTPLL
110 120 130 140 150
RMKDGKFHKE GDFTPVSWDQ AFTIMAEKVK DILKKKEPNS VGMFSSGQTT
160 170 180 190 200
IFEGYAKVKL WKGGLRSNTI DPNARHCMAS AAVAFMRTFG MDEPMGCYND
210 220 230 240 250
IEKTDAFVLW GSNMAEMHPI LWSRISDRRL SDDKVKVVVM STFEHRSFEL
260 270 280 290 300
ADTPIIFKPH SDLAILNYIA NYIIQNDKVN WDFVNKHTKF KRGETDIGYG
310 320 330 340 350
LRPDHPRQKA AKNAKTAGKM YDSDFEEFKK IVEPYTLEKA HEISGVPKDQ
360 370 380 390 400
LETLAKMYAD PKQTLVSFWT MGFNQHVRGV WVNHMVYNVH LLTGKISTPG
410 420 430 440 450
CGPFSLTGQP SACGTAREVG TFVHRLPADM VVTNPKHVEI VEKAWKLPKG
460 470 480 490 500
TIPTVPGYPA VMQSRMLKDG KLNFLWQLCT NNMQGGPNIN EEIFPGWRNP
510 520 530 540 550
ENFIVVSDPY PSVSAVAADL ILPTCMWVEK EGAYGNAERR TQFWRQQVKG
560 570 580 590 600
PGESKSDLWQ IVEFSKYFKT DEVWDEALLA QMPEYRGKTL YEVLYKNGQV
610 620 630 640 650
DKFDTPTNIP GYINDEAEHF GYYLQKGLFE EYAAFGRGHG HDLADFDTYH
660 670 680 690 700
QVRGLRWPVV DGKETLWRYR EGFDPYVKAG EGVSFYGYPD KKAIILGVPY
710 720 730 740 750
EEPAESPDEE YDLWLCTGRV LEHWHTGTMT RRVPELHRSF PNNLCWMHPD
760 770 780 790 800
DAKARGLRHG DKVKLITRRG EIITHLDTRG RNKCPKGLIY TTFFDAGQLA
810 820
NKLTLDATDP ISGETDYKKC AVKVVKA
Length:827
Mass (Da):93,572
Last modified:February 26, 2008 - v1
Checksum:iBE020AD5642AF456
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000687 Genomic DNA. Translation: ABY70013.1.
RefSeqiYP_001652457.1. NC_010278.1.

Genome annotation databases

EnsemblBacteriaiABY70013; ABY70013; APJL_1461.
GeneIDi5850761.
KEGGiapj:APJL_1461.
PATRICi20752207. VBIActPle136345_1454.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000687 Genomic DNA. Translation: ABY70013.1 .
RefSeqi YP_001652457.1. NC_010278.1.

3D structure databases

ProteinModelPortali B0BR28.
SMRi B0BR28. Positions 35-826.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 434271.APJL_1461.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABY70013 ; ABY70013 ; APJL_1461 .
GeneIDi 5850761.
KEGGi apj:APJL_1461.
PATRICi 20752207. VBIActPle136345_1454.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi AGKMHDS.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci APLE434271:GIX7-1480-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China."
    Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T.
    , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H.
    PLoS ONE 3:E1450-E1450(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JL03.

Entry informationi

Entry nameiNAPA_ACTPJ
AccessioniPrimary (citable) accession number: B0BR28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 26, 2008
Last modified: October 29, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3