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B0BR28

- NAPA_ACTPJ

UniProt

B0BR28 - NAPA_ACTPJ

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Actinobacillus pleuropneumoniae serotype 3 (strain JL03)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (26 Feb 2008)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi44 – 441Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi79 – 791Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciAPLE434271:GIX7-1480-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:APJL_1461
    OrganismiActinobacillus pleuropneumoniae serotype 3 (strain JL03)
    Taxonomic identifieri434271 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
    ProteomesiUP000008547: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
    BLAST
    Chaini33 – 827795Periplasmic nitrate reductasePRO_1000186349Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi434271.APJL_1461.

    Structurei

    3D structure databases

    ProteinModelPortaliB0BR28.
    SMRiB0BR28. Positions 35-826.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 93574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiAGKMHDS.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B0BR28-1 [UniParc]FASTAAdd to Basket

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    MELNRRDFMK ANAAMAAAAA AGMTIPVKNV YAADDGIRWD KAPCRFCGTG    50
    CSVLVGTKDG RVVATQGDPD AEVNRGLNCI KGYFLSKIMY GADRVQTPLL 100
    RMKDGKFHKE GDFTPVSWDQ AFTIMAEKVK DILKKKEPNS VGMFSSGQTT 150
    IFEGYAKVKL WKGGLRSNTI DPNARHCMAS AAVAFMRTFG MDEPMGCYND 200
    IEKTDAFVLW GSNMAEMHPI LWSRISDRRL SDDKVKVVVM STFEHRSFEL 250
    ADTPIIFKPH SDLAILNYIA NYIIQNDKVN WDFVNKHTKF KRGETDIGYG 300
    LRPDHPRQKA AKNAKTAGKM YDSDFEEFKK IVEPYTLEKA HEISGVPKDQ 350
    LETLAKMYAD PKQTLVSFWT MGFNQHVRGV WVNHMVYNVH LLTGKISTPG 400
    CGPFSLTGQP SACGTAREVG TFVHRLPADM VVTNPKHVEI VEKAWKLPKG 450
    TIPTVPGYPA VMQSRMLKDG KLNFLWQLCT NNMQGGPNIN EEIFPGWRNP 500
    ENFIVVSDPY PSVSAVAADL ILPTCMWVEK EGAYGNAERR TQFWRQQVKG 550
    PGESKSDLWQ IVEFSKYFKT DEVWDEALLA QMPEYRGKTL YEVLYKNGQV 600
    DKFDTPTNIP GYINDEAEHF GYYLQKGLFE EYAAFGRGHG HDLADFDTYH 650
    QVRGLRWPVV DGKETLWRYR EGFDPYVKAG EGVSFYGYPD KKAIILGVPY 700
    EEPAESPDEE YDLWLCTGRV LEHWHTGTMT RRVPELHRSF PNNLCWMHPD 750
    DAKARGLRHG DKVKLITRRG EIITHLDTRG RNKCPKGLIY TTFFDAGQLA 800
    NKLTLDATDP ISGETDYKKC AVKVVKA 827
    Length:827
    Mass (Da):93,572
    Last modified:February 26, 2008 - v1
    Checksum:iBE020AD5642AF456
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000687 Genomic DNA. Translation: ABY70013.1.
    RefSeqiYP_001652457.1. NC_010278.1.

    Genome annotation databases

    EnsemblBacteriaiABY70013; ABY70013; APJL_1461.
    GeneIDi5850761.
    KEGGiapj:APJL_1461.
    PATRICi20752207. VBIActPle136345_1454.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000687 Genomic DNA. Translation: ABY70013.1 .
    RefSeqi YP_001652457.1. NC_010278.1.

    3D structure databases

    ProteinModelPortali B0BR28.
    SMRi B0BR28. Positions 35-826.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 434271.APJL_1461.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABY70013 ; ABY70013 ; APJL_1461 .
    GeneIDi 5850761.
    KEGGi apj:APJL_1461.
    PATRICi 20752207. VBIActPle136345_1454.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi AGKMHDS.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci APLE434271:GIX7-1480-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China."
      Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T.
      , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H.
      PLoS ONE 3:E1450-E1450(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JL03.

    Entry informationi

    Entry nameiNAPA_ACTPJ
    AccessioniPrimary (citable) accession number: B0BR28
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: February 26, 2008
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3