B0BR08 (B0BR08_ACTPJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutathione biosynthesis bifunctional protein GshAB HAMAP-Rule MF_00782

Alternative name(s):
Gamma-GCS-GS HAMAP-Rule MF_00782

Gene names

Name:	gshA EMBL ABY69993.1
Synonyms:	gshAB HAMAP-Rule MF_00782, gshF HAMAP-Rule MF_00782
Ordered Locus Names:	APJL_1439

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP] EMBL ABY69993.1

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus › Actinobacillus pleuropneumoniae › Actinobacillus pleuropneumoniae serovar 3

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine By similarity. HAMAP-Rule MF_00782
Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP-Rule MF_00782 ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00782
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP-Rule MF_00782 Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00782
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00782
Sequence similarities	Contains 1 ATP-grasp domain. HAMAP-Rule MF_00782 In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily. HAMAP-Rule MF_00782

Ontologies

Keywords
Biological process	Glutathione biosynthesis HAMAP-Rule MF_00782
Ligand	ATP-binding HAMAP-Rule MF_00782 Magnesium HAMAP-Rule MF_00782 Manganese HAMAP-Rule MF_00782 Metal-binding HAMAP-Rule MF_00782 Nucleotide-binding
Molecular function	Ligase HAMAP-Rule MF_00782
Technical term	Complete proteome Multifunctional enzyme HAMAP-Rule MF_00782
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-cysteine ligase activity Inferred from electronic annotation. Source: HAMAP glutathione synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Domain	494 – 753	260	ATP-grasp By similarity HAMAP-Rule MF_00782
	Nucleotide binding	521 – 580	60	ATP By similarity HAMAP-Rule MF_00782
	Region	1 – 337	337	Glutamate--cysteine ligase By similarity HAMAP-Rule MF_00782
Sites
	Metal binding	702	1	Magnesium or manganese 1 By similarity HAMAP-Rule MF_00782
	Metal binding	723	1	Magnesium or manganese 1 By similarity HAMAP-Rule MF_00782
	Metal binding	723	1	Magnesium or manganese 2 By similarity HAMAP-Rule MF_00782
	Metal binding	725	1	Magnesium or manganese 2 By similarity HAMAP-Rule MF_00782

Sequences

	Sequence		Length	Mass (Da)
	B0BR08 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: C13B124E7E70A8D0	FASTA	757	85,384
	10 20 30 40 50 60 MKLQQLIKTH HLGLLFQQGK FGIEKESQRI DNKGNIVTTA HPSVFGNRSY HPYIQTDFAE 70 80 90 100 110 120 SQLELITPPN DKLENTYRWL SAIHEVTLRS LPDDEYIFPF SMPAGLPPES EIKEAQLDNE 130 140 150 160 170 180 WDVKYREHLS AIYGKYKQMV SGIHYNFQIS DEFVESAFAL QTEYPNKIAF RNALYMKLAN 190 200 210 220 230 240 NFLRYQWILV YLLAATPTVE AQYFGENRPL AEGQLVRSLR SGPYGYVNAP HIVINHDSLQ 250 260 270 280 290 300 QYVESLEHFV ATGDLLAEKE FYSNVRLRGA KKARELLEKG VKYAEFRLFD LNPFSPYGIE 310 320 330 340 350 360 LADAKFIHLF LLAMLWMDET SGQREVEIGT QKLYQVALED PRSHTAFQAE GEAILNLMLA 370 380 390 400 410 420 MLDDLSVPQN EKDLLQQKLA QFADPSQTVN GRLLAAIEQA GSYKALGAQL AQQYKAQAFE 430 440 450 460 470 480 RFYAISAFDN MELSTQALLF DAIQQGLQIE LLDENDQFLA LKFGDHLEYV KNGNMTSHDQ 490 500 510 520 530 540 YISPLIMENK VVTKKVLAKA GFNVPKSIEF TSVEQAVAHY PLFEGKAMVI KPKSTNYGLG 550 560 570 580 590 600 ITIFQQGVTD KADFAKAIEI AFREDKEVMV EDYLVGTEYR FFVLGDETLA VLLRVPANVK 610 620 630 640 650 660 GDGIHTVREL VEAKNSDPLR GDGSRSPLKK IALGDIELLQ LKEQGLTPDS IPADGQIVQL 670 680 690 700 710 720 RANSNISTGG DSIDMTDQMH DSYKQLAVGI AKEMGAKVCG VDLIIPDLTK AAEPSLRSWG 730 740 750 VIEANFNPMM MMHIFPYQGK SRRLTKAVLK MLFPELP

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JL03.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000687 Genomic DNA. Translation: ABY69993.1.
RefSeq	YP_001652437.1. NC_010278.1.
3D structure databases
ProteinModelPortal	B0BR08.
ModBase	Search...
Protein-protein interaction databases
STRING	434271.APJL_1439.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABY69993; ABY69993; APJL_1439.
GeneID	5851148.
KEGG	apj:APJL_1439.
PATRIC	20752160. VBIActPle136345_1431.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1181.
HOGENOM	HOG000156471.
KO	K01919.
OMA	PYIQTDF.
ProtClustDB	PRK02471.
Enzyme and pathway databases
BioCyc	APLE434271:GIX7-1459-MONOMER.
UniPathway	UPA00142; UER00209. UPA00142; UER00210.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 3 hits.
HAMAP	MF_00782. Glut_biosynth.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR007370. Glu_cys_ligase. IPR006335. Glut_cys-rel. [Graphical view]
Pfam	PF04262. Glu_cys_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01435. glu_cys_lig_rel. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B0BR08_ACTPJ

Accession

Primary (citable) accession number: B0BR08

Entry history

Integrated into UniProtKB/TrEMBL:	February 26, 2008
Last sequence update:	February 26, 2008
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)