Reviewed, UniProtKB/Swiss-Prot B0BQ90 (SYD_ACTPJ)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	APJL_1169

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity. HAMAP MF_00044
Subcellular location	Cytoplasm By similarity HAMAP MF_00044.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_1000090953

Sequences

	Sequence		Length	Mass (Da)
	B0BQ90-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 3CFE6D25A9076D8B	FASTA	591	66,809
	10 20 30 40 50 60 MMRSHYCGAL NRSHVGQTVT LSGWVHRVRN LGRFIFMQIR DREGIVQVFF DEKDEAIFKI 70 80 90 100 110 120 ASSLRSEACV QIQGEVIARD ESQINKEMAT GEIEVLVKNV VVYNNADVLP LDFNQNNTEE 130 140 150 160 170 180 QRLKYRYLDL RRPEMAEKLK TRAKITSFVR RYMDDNGFLD IETPMLTKAT PEGARDYLVP 190 200 210 220 230 240 SRVHNGKFYA LPQSPQLFKQ LLMMSGFDRY YQIVKCFRDE DLRADRQPEF TQIDVETSFL 250 260 270 280 290 300 TAEEVRELME NMIHGLWLDR LNVDLGKFPI MTWQEAMQRF GSDKPDLRNP LELVDVADIL 310 320 330 340 350 360 KDVEFKVFNE PANSADGRVT VLRVPNGASL TRKQIDEYTQ FVGIYGAKGL AWAKINNVNA 370 380 390 400 410 420 GMEGIQSPVA KFLNEEVFKA LIERTNATSG DILFFGADKW QVVTDSMGAL RLKVGRDLAL 430 440 450 460 470 480 TDLSAWKPLW VIDFPMFEKD DEGNLSAMHH PFTSPKNLTP EELAANPVNA VANAYDMVIN 490 500 510 520 530 540 GYEVGGGSVR IYDPKMQQTV FGILGINEQD QQEKFGFLLD ALKFGTPPHA GLAFGLDRLT 550 560 570 580 590 MLITGTENIR DVIAFPKTTA AACLMTEAPS FANPQALEEL GIAVLKKEKA E

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000687 Genomic DNA. Translation: ABY69725.1.
RefSeq	YP_001652169.1.
3D structure databases
SMR	B0BQ90. Positions 2-587.
ModBase	Search...
Genome annotation databases
GeneID	5850872.
GenomeReviews	Gene locus APJL_1169 in contig CP000687_GR.
KEGG	apj:APJL_1169.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	PLFEYDE.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR002312. Asp-tRNA-synth_IIb. IPR020564. Asp-tRNA-synth_IIb_bac-type. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_ACTPJ

Accession

Primary (citable) accession number: B0BQ90

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 26, 2008
Last modified:	February 9, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)