B0BQ51 (NAGZ_ACTPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-hexosaminidase
EC=3.2.1.52

Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase

Gene names

Name:	nagZ
Ordered Locus Names:	APJL_1130

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus › Actinobacillus pleuropneumoniae › Actinobacillus pleuropneumoniae serovar 3

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides By similarity. HAMAP-Rule MF_00364
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP-Rule MF_00364
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_00364
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Beta-hexosaminidase HAMAP-Rule MF_00364		PRO_1000121052
Sites
	Active site	247	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0BQ51 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 47C95172D84F3CC5	FASTA	345	38,994
	10 20 30 40 50 60 MLLIDIKDKE LSQEEVEILE HPLVSGLILF SRNFHDKVQL EALVKSIRQR VKKPLLITVD 70 80 90 100 110 120 QEGGRVQRFR EGFTKLPAMQ AFHTLAKNPQ ESTALARQTG WLMAAEMFAL DIDLSFAPVL 130 140 150 160 170 180 DLGHQCKAIG DRSFGENPDA MLPIAEAFID GMREMGMATT GKHFPGHGHV LADSHLETPF 190 200 210 220 230 240 DDRPKEAIFN RDILPFKQLI SKGKLSAVMP AHVIYTQCDS QPASGSEYWL KQVLRNQLNF 250 260 270 280 290 300 NGVIFSDDLG MKGAGFMGNF VERSEKAIHA GCDLLLLCNE PEGVIQVLDG LKYQPTKTQT 310 320 330 340 ERHISLMKRK TVRWNELEAS PRYQQAQQRL TALQNEWLEY KAQHC

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JL03.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000687 Genomic DNA. Translation: ABY69686.1.
RefSeq	YP_001652130.1. NC_010278.1.
3D structure databases
ProteinModelPortal	B0BQ51.
ModBase	Search...
Protein-protein interaction databases
STRING	434271.APJL_1130.
Protein family/group databases
CAZy	GH3. Glycoside Hydrolase Family 3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABY69686; ABY69686; APJL_1130.
GeneID	5852189.
KEGG	apj:APJL_1130.
PATRIC	20751496. VBIActPle136345_1124.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1472.
HOGENOM	HOG000248526.
KO	K01207.
OMA	VVLHCNG.
ProtClustDB	PRK05337.
Enzyme and pathway databases
BioCyc	APLE434271:GIX7-1126-MONOMER.
UniPathway	UPA00544.
Family and domain databases
Gene3D	3.20.20.300. 1 hit.
HAMAP	MF_00364. NagZ.
InterPro	IPR022956. Beta_hexosaminidase_bac. IPR019800. Glyco_hydro_3_AS. IPR001764. Glyco_hydro_3_N. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00933. Glyco_hydro_3. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGZ_ACTPJ

Accession

Primary (citable) accession number: B0BQ51

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 26, 2008
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program