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Protein

Beta-hexosaminidase

Gene

nagZ

Organism
Actinobacillus pleuropneumoniae serotype 3 (strain JL03)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides.UniRule annotation

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei60 – 601SubstrateUniRule annotation
Binding sitei68 – 681SubstrateUniRule annotation
Binding sitei132 – 1321SubstrateUniRule annotation
Sitei173 – 1731Important for catalytic activityUniRule annotation
Active sitei175 – 1751Proton donor/acceptorUniRule annotation
Active sitei247 – 2471NucleophileUniRule annotation

GO - Molecular functioni

  1. beta-N-acetylhexosaminidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cell cycle Source: UniProtKB-KW
  3. cell division Source: UniProtKB-KW
  4. cell wall organization Source: UniProtKB-KW
  5. peptidoglycan biosynthetic process Source: UniProtKB-KW
  6. peptidoglycan turnover Source: UniProtKB-HAMAP
  7. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciAPLE434271:GIX7-1126-MONOMER.
UniPathwayiUPA00544.

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-hexosaminidaseUniRule annotation (EC:3.2.1.52UniRule annotation)
Alternative name(s):
Beta-N-acetylhexosaminidaseUniRule annotation
N-acetyl-beta-glucosaminidaseUniRule annotation
Gene namesi
Name:nagZUniRule annotation
Ordered Locus Names:APJL_1130
OrganismiActinobacillus pleuropneumoniae serotype 3 (strain JL03)
Taxonomic identifieri434271 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
ProteomesiUP000008547: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Beta-hexosaminidasePRO_1000121052Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi434271.APJL_1130.

Structurei

3D structure databases

ProteinModelPortaliB0BQ51.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni162 – 1632Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1472.
HOGENOMiHOG000248526.
KOiK01207.
OMAiHILALYE.
OrthoDBiEOG6BCT06.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
HAMAPiMF_00364. NagZ.
InterProiIPR022956. Beta_hexosaminidase_bac.
IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0BQ51-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLIDIKDKE LSQEEVEILE HPLVSGLILF SRNFHDKVQL EALVKSIRQR
60 70 80 90 100
VKKPLLITVD QEGGRVQRFR EGFTKLPAMQ AFHTLAKNPQ ESTALARQTG
110 120 130 140 150
WLMAAEMFAL DIDLSFAPVL DLGHQCKAIG DRSFGENPDA MLPIAEAFID
160 170 180 190 200
GMREMGMATT GKHFPGHGHV LADSHLETPF DDRPKEAIFN RDILPFKQLI
210 220 230 240 250
SKGKLSAVMP AHVIYTQCDS QPASGSEYWL KQVLRNQLNF NGVIFSDDLG
260 270 280 290 300
MKGAGFMGNF VERSEKAIHA GCDLLLLCNE PEGVIQVLDG LKYQPTKTQT
310 320 330 340
ERHISLMKRK TVRWNELEAS PRYQQAQQRL TALQNEWLEY KAQHC
Length:345
Mass (Da):38,994
Last modified:February 26, 2008 - v1
Checksum:i47C95172D84F3CC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000687 Genomic DNA. Translation: ABY69686.1.
RefSeqiWP_012263104.1. NC_010278.1.
YP_001652130.1. NC_010278.1.

Genome annotation databases

EnsemblBacteriaiABY69686; ABY69686; APJL_1130.
GeneIDi5852189.
KEGGiapj:APJL_1130.
PATRICi20751496. VBIActPle136345_1124.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000687 Genomic DNA. Translation: ABY69686.1.
RefSeqiWP_012263104.1. NC_010278.1.
YP_001652130.1. NC_010278.1.

3D structure databases

ProteinModelPortaliB0BQ51.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi434271.APJL_1130.

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY69686; ABY69686; APJL_1130.
GeneIDi5852189.
KEGGiapj:APJL_1130.
PATRICi20751496. VBIActPle136345_1124.

Phylogenomic databases

eggNOGiCOG1472.
HOGENOMiHOG000248526.
KOiK01207.
OMAiHILALYE.
OrthoDBiEOG6BCT06.

Enzyme and pathway databases

UniPathwayiUPA00544.
BioCyciAPLE434271:GIX7-1126-MONOMER.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
HAMAPiMF_00364. NagZ.
InterProiIPR022956. Beta_hexosaminidase_bac.
IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China."
    Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T.
    , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H.
    PLoS ONE 3:E1450-E1450(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JL03.

Entry informationi

Entry nameiNAGZ_ACTPJ
AccessioniPrimary (citable) accession number: B0BQ51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 26, 2008
Last modified: February 4, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.