Reviewed, UniProtKB/Swiss-Prot B0BPJ8 (SYC_ACTPJ)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	APJL_0925

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Cysteinyl-tRNA synthetase HAMAP MF_00041		PRO_1000090813
Regions
	Motif	30 – 40	11	"HIGH" region HAMAP MF_00041
	Motif	266 – 270	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	28	1	Zinc By similarity
	Metal binding	209	1	Zinc By similarity
	Metal binding	234	1	Zinc By similarity
	Metal binding	238	1	Zinc By similarity
	Binding site	269	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0BPJ8-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: A2C4006AB0B031C2	FASTA	459	52,162
	10 20 30 40 50 60 MLKIYNTLKR EKEEFKPINP NQVGMYVCGV TVYDLCHFGH GRTFVSFDVI ARYLRYLGYN 70 80 90 100 110 120 LRYVRNITDV DDKIIKRALE NNETCDQLVD RMIAEMHKDF DDLNILRPDV EPRATKHIPE 130 140 150 160 170 180 IVAMVEKLIA NGHAYVAADG DVMFDVESFK KYGALSRQNL EQLQAGARVE IKSVKKNPMD 190 200 210 220 230 240 FVLWKMSKEG EPSWQSPWGN GRPGWHIECS AMNSKELGEH FDIHGGGSDL MFPHHENEIA 250 260 270 280 290 300 QSCCAHGGDY VNYWLHTGML TIDDEKMSKS LGNFFTIRTM LEKYESETLR YFFLTAHYRS 310 320 330 340 350 360 LLNYSLNNLD LARSALERLY TSLRGCDLSV EVAGGEQYVE AFKAAMDDDF NTPGALAVLF 370 380 390 400 410 420 EIAREVNKLK TEDMAKANGL AVRLKELAGV LGLLYQDPEA FLQGDADNDE VAEIEALIKQ 430 440 450 RNEAKAAKNW AVADEVRDKL KAMNIVLEDT PNGTTWRKA

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000687 Genomic DNA. Translation: ABY69483.1.
RefSeq	YP_001651927.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5852396.
GenomeReviews	Gene locus APJL_0925 in contig CP000687_GR.
KEGG	apj:APJL_0925.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VLWKAAK.
Family and domain databases
HAMAP	MF_00041. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ACTPJ

Accession

Primary (citable) accession number: B0BPJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 26, 2008
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)