Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B0BPF7 (SYL_ACTPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:APJL_0884
OrganismActinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]
Taxonomic identifier434271 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Protein attributes

Sequence length861 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 861861Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091283

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B0BPF7 [UniParc].

Last modified February 26, 2008. Version 1.
Checksum: 3F8A8665AFF303D5

FASTA86197,751
        10         20         30         40         50         60 
MQQQYNPSAI EPKVQQFWAE NKVFKAVKDV TKEKYYCLSM LPYPSGKLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VVSRYQRMIG KNVLQPMGWD AFGLPAEGAA VKNNTAPAKW TYENIEYMKG QLKMLGFSYD 

       130        140        150        160        170        180 
WDREVTTCRP EYYKWEQWFF TELYKKGLVY KKTSTVNWCP NDATVLANEQ VHEGCCWRCD 

       190        200        210        220        230        240 
TPVEQREIPQ WFIKITDYAE ELLTHLDNLP QWPDQVKTMQ RNWIGRSEGV EITFKIAGSN 

       250        260        270        280        290        300 
AELPVYTTRP DTFFGVSYVA IAAAHPLAEM AAENNPALAE FIREAKNTKV AEAELATMEK 

       310        320        330        340        350        360 
KGMATGLFAI HPLTGKEVPV WVANFVLMHY GTGAVMAVPA HDERDFEFAQ KYGLQINQVI 

       370        380        390        400        410        420 
QPLDGSEWDF SKAAYTEHGK LINSAEFDDL NFEQAFNAIA DKLESMKVGK RQVNFRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMMTTEDG EVVTVPMQDL PVILPEDVVM NGVQSPIKAD PEWAKTTYNG 

       490        500        510        520        530        540 
KPALKETDTF DTFMESSWYY ARYTCPQYHE GMLDSDEANY WLPVDQYIGG IEHATMHLLY 

       550        560        570        580        590        600 
FRFFHKLLRD AGILNSDEPA TKLLCQGMVL ADAFYYTSPT NERIWVSPTQ VTLERDEKGR 

       610        620        630        640        650        660 
IIKATDPEGR ELVHSGMTKM SKSKNNGIDP QEMVEKYGAD TVRLFMMFAS PAEMTLEWQE 

       670        680        690        700        710        720 
SGVEGAKRFL GRVWNLVYEY SQNPATAALD VAALSKAQKE LRRDVHKTIA KVSDDIGRRQ 

       730        740        750        760        770        780 
TFNTAIAAIM ELMNKLTKAP LENEQDKAVM AEALSAVVRM LYPITPHICF ELWQALGNND 

       790        800        810        820        830        840 
TIDFAPWVVA DESAMVEDEK LVVVQVNGKV RGKVTVSATA TEDEVKAIAK ADANVAKFLE 

       850        860 
GVEIVKEIYV PYKMLSFAVK A 

« Hide

References

[1]"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China."
Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. expand/collapse author list , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H.
PLoS ONE 3:E1450-E1450(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JL03.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000687 Genomic DNA. Translation: ABY69442.1.
RefSeqYP_001651886.1. NC_010278.1.

3D structure databases

ProteinModelPortalB0BPF7.
SMRB0BPF7. Positions 228-413.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING434271.APJL_0884.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY69442; ABY69442; APJL_0884.
GeneID5852448.
KEGGapj:APJL_0884.
PATRIC20751012. VBIActPle136345_0884.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycAPLE434271:GIX7-879-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ACTPJ
AccessionPrimary (citable) accession number: B0BPF7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: February 26, 2008
Last modified: April 16, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries