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Protein

DNA topoisomerase 3

Gene

topB2

Organism
Actinobacillus pleuropneumoniae serotype 3 (strain JL03)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71Magnesium 1; catalyticUniRule annotation
Metal bindingi103 – 1031Magnesium 1; catalyticUniRule annotation
Metal bindingi103 – 1031Magnesium 2UniRule annotation
Metal bindingi105 – 1051Magnesium 2UniRule annotation
Active sitei329 – 3291O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, TopoisomeraseUniRule annotationSAAS annotation

Keywords - Ligandi

DNA-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciAPLE434271:GIX7-739-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topB2Imported
Synonyms:topBUniRule annotation
Ordered Locus Names:APJL_0746Imported
OrganismiActinobacillus pleuropneumoniae serotype 3 (strain JL03)Imported
Taxonomic identifieri434271 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
Proteomesi
  • UP000008547 Componenti: Chromosome

Interactioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei61 – 611Interaction with DNAUniRule annotation
Sitei170 – 1701Interaction with DNAUniRule annotation
Sitei178 – 1781Interaction with DNAUniRule annotation
Sitei185 – 1851Interaction with DNAUniRule annotation
Sitei331 – 3311Interaction with DNAUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB0BP24.
SMRiB0BP24. Positions 1-609.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 134134ToprimInterPro annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni194 – 1996Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotationSAAS annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000086848.
KOiK03169.
OMAiANQRYGF.
OrthoDBiEOG60CWM6.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 2 hits.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0BP24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVFLAEKPS LARAIADVLP KPHTRGDGYI QCGENDTVTW CIGHLLEQAE
60 70 80 90 100
PDIYDERFKM WRMEHLPIVP QQWKLVPKKE TLKQLKAVEK LVKKADVLVN
110 120 130 140 150
AGDPDREGQL LVDEVFSYLN VPPEKRNRIQ RCLVSDLNPS AVKKAIDKLQ
160 170 180 190 200
NNRDFIPLAT SALARARADW LYGINMTRAY TLQGRWAGYQ GVLSVGRVQT
210 220 230 240 250
PVLGLIVRRD LEIENFVPKD YFEVLAHIVV PETQERFTAQ WQPSKACEDY
260 270 280 290 300
QDEDGRVLSK SLAENVVKRI TDQAALVTDY QDKIETETAP LPYSLSVLQI
310 320 330 340 350
DAARRYGLSA QAVLDICQKL YETHKLITYP RSDNRYLPNE HYADRFKVMA
360 370 380 390 400
AIAHHLDEYK EKPEAVNPEI RNRCWNDKKV EAHHAIIPTV RQGSVKLTEN
410 420 430 440 450
EKRIYQLIAR QYLMQFCPDA EYRKGKIVLE IAGGTFNAQA RNLIVAGWKA
460 470 480 490 500
LLGKEDSDEV LEPLLPVVKK GQQLHCEKGE IVSKKTQPPR HFTDASLLSA
510 520 530 540 550
MTGIARFVQD KQLKKVLRET DGLGTEATRA GIIELLFKRG FLIKKGRNIH
560 570 580 590 600
STEAGRILIS ALPDEATQPD MTAHWEMQLT DISKKQASYQ QFMQVLTSKL
610 620 630
PELLHTERQK LQNLSRIQPP ASYRKRSNFK KNLQTAEK
Length:638
Mass (Da):72,884
Last modified:February 26, 2008 - v1
Checksum:iEC81EFD543D000E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000687 Genomic DNA. Translation: ABY69309.1.
RefSeqiWP_005607634.1. NC_010278.1.

Genome annotation databases

EnsemblBacteriaiABY69309; ABY69309; APJL_0746.
GeneIDi4848998.
KEGGiapj:APJL_0746.
PATRICi20750733. VBIActPle136345_0750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000687 Genomic DNA. Translation: ABY69309.1.
RefSeqiWP_005607634.1. NC_010278.1.

3D structure databases

ProteinModelPortaliB0BP24.
SMRiB0BP24. Positions 1-609.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY69309; ABY69309; APJL_0746.
GeneIDi4848998.
KEGGiapj:APJL_0746.
PATRICi20750733. VBIActPle136345_0750.

Phylogenomic databases

HOGENOMiHOG000086848.
KOiK03169.
OMAiANQRYGF.
OrthoDBiEOG60CWM6.

Enzyme and pathway databases

BioCyciAPLE434271:GIX7-739-MONOMER.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 2 hits.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China."
    Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T.
    , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H.
    PLoS ONE 3:E1450-E1450(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JL03Imported.

Entry informationi

Entry nameiB0BP24_ACTPJ
AccessioniPrimary (citable) accession number: B0BP24
Entry historyi
Integrated into UniProtKB/TrEMBL: February 26, 2008
Last sequence update: February 26, 2008
Last modified: July 6, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.