B0BNY1 (AROA_ACTPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	APJL_0697

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000099660

Sequences

	Sequence		Length	Mass (Da)
	B0BNY1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 6F96F8055D1DE572	FASTA	432	46,908
	10 20 30 40 50 60 MEKITLAPIS RVEGEINLPG SKSLSNRALL LAALAKGTTK VTNLLDSDDI RHMLNALKAL 70 80 90 100 110 120 GVNYSLSEDK TVCTVEGVGG AFNWKNGLAL FLGNAGTAMR PLTAALCLKG ATEAEVVLTG 130 140 150 160 170 180 EPRMKERPIK HLVDALRQAG ASVQYLENEG YPPVAIRNSG LKGGKVQIDG SISSQFLTAL 190 200 210 220 230 240 LMAAPLAEGD MEIEIIGELV SKPYIDITLA MMKDFGVKVE NRNYQTFVVK GNQSYLSPEK 250 260 270 280 290 300 YLVEGDASSA SYFLAAGAIK GKVKVTGIGK NSIQGDRLFA NVLEAMGAKI TWGDDFIQAE 310 320 330 340 350 360 QGKLKGVDMD MNHIPDAAMT IATAALFAEG ETVIRNIYNW RVKETDRLTA MATELRKVGA 370 380 390 400 410 420 TVEEGEDFIR IQPLPLTQFQ HAEIATYNDH RMAMCFSLIA LSDTPVTILD PKCTAKTFPT 430 YFTEFEKLSE RT

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JL03.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000687 Genomic DNA. Translation: ABY69266.1.
RefSeq	YP_001651710.1. NC_010278.1.
3D structure databases
ProteinModelPortal	B0BNY1.
ModBase	Search...
Protein-protein interaction databases
STRING	B0BNY1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5852699.
GenomeReviews	Gene locus APJL_0697 in contig CP000687_GR.
KEGG	apj:APJL_0697.
PATRIC	20750632. VBIActPle136345_0701.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646626.
OMA	GADIEWG.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	APLE434271:APJL_0697-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_ACTPJ

Accession

Primary (citable) accession number: B0BNY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 26, 2008
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program