Reviewed, UniProtKB/Swiss-Prot B0BNS8 (SYA_ACTPJ)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	APJL_0644

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 874	874	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347476

Sequences

	Sequence		Length	Mass (Da)
	B0BNS8-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: AE6613FF70B97D9D	FASTA	874	96,370
	10 20 30 40 50 60 MKTTSEIRQS FLDFFHSKGH TVVPSSSLVP ENDPTLLFTN AGMNQFKDVF LGLEKRPYTR 70 80 90 100 110 120 ATTAQRCVRA GGKHNDLENV GYTARHHTFF EMMGNFSFGD YFKHDAIQFG WEYLTSPQWL 130 140 150 160 170 180 GLPKEKLYVT VYETDDEAYD IWNKIVGVPT DHIIRIGDNK GAPYASDNFW AMGDTGPCGP 190 200 210 220 230 240 CTEIFYDHGE TFWGGLPGSP EEDGDRYIEV WNIVFMQFNR LADGTMEKLP KPSVDTGMGL 250 260 270 280 290 300 ERMTAVMQHV NSNYETDIFQ TLIKEVAGLL NVSDLDNKSL RVVADHIRAC SYLIADGVVP 310 320 330 340 350 360 SNEGRGYVLR RIIRRAVRHG NLLGAKEAFF YKLVPTLATV MGHAGEVLTQ KQAHIQKTLK 370 380 390 400 410 420 AEEEQFARTL ERGLALLEDA LTKVENNTLS GEVAFKLYDT YGFPLDLTAD VCRERELTID 430 440 450 460 470 480 EAGFEAEMTA QRERAKASSN FGTDYNNVIK VEGQTDFIGY DNLEAQATIV GLFSNGKAVD 490 500 510 520 530 540 TIQSGESAVI ILDQTPFYAE MGGQVGDSGL ISTEICNFTV NDTQKYGQVF GHIGQLTSGS 550 560 570 580 590 600 LSIGDKVTAT VHATRRIAIT ANHSATHLLH SALREVLGDH VAQKGSLVSE NILRFDFSQP 610 620 630 640 650 660 EAISKSQLEE IERIVNRKIR ENIQVTIETM DIESAKKKGA MALFGEKYGD VVRVVGMTEF 670 680 690 700 710 720 SIELCGGTHV QRTGDIGLFK LVSEGAVAAG IRRVEAVTAE TAIEWLHNQQ KVLQQSAEFL 730 740 750 760 770 780 KADSNSLVEK IQQLQDKAKR TEKELQQLKD KLAAQAGSEL VKQANKINGV NVVVQKLENV 790 800 810 820 830 840 EVKSLRTMVD DLKNQLESAI VVFGTVADEK VNLIVGVTKD LSSKVNAGEL VGAMAQQVGG 850 860 870 KGGGRADMAM AGGSEPQNLD NALKFAEEWI QAKL

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000687 Genomic DNA. Translation: ABY69213.1.
RefSeq	YP_001651657.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5852763.
GenomeReviews	Gene locus APJL_0644 in contig CP000687_GR.
KEGG	apj:APJL_0644.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TLMFTNS.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ACTPJ

Accession

Primary (citable) accession number: B0BNS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 26, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)