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Protein

NAD(P)H-hydrate epimerase

Gene

Naxe

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.UniRule annotationBy similarity

Catalytic activityi

(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi114PotassiumUniRule annotation1
Metal bindingi179PotassiumUniRule annotation1
Binding sitei212NAD(P)HXUniRule annotation1
Metal bindingi215PotassiumUniRule annotation1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • NADHX epimerase activity Source: MGI
  • NADPHX epimerase activity Source: MGI
  • nucleotide binding Source: UniProtKB-KW
  • protein homodimerization activity Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionIsomerase
LigandMetal-binding, NAD, NADP, Nucleotide-binding, Potassium

Enzyme and pathway databases

BRENDAi5.1.99.6. 5301.
ReactomeiR-RNO-197264. Nicotinamide salvaging.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H-hydrate epimeraseUniRule annotation (EC:5.1.99.6)
Alternative name(s):
Apolipoprotein A-I-binding proteinUniRule annotation
Short name:
AI-BPUniRule annotation
NAD(P)HX epimeraseImported
Gene namesi
Name:NaxeImported
Synonyms:Aibp, Apoa1bp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1304699. Naxe.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 53MitochondrionUniRule annotationAdd BLAST53
ChainiPRO_000041631054 – 282NAD(P)H-hydrate epimeraseAdd BLAST229

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei138N6-succinyllysineBy similarity1

Post-translational modificationi

Undergoes physiological phosphorylation during sperm capacitation, downstream to PKA activation.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiB0BNM1.
PeptideAtlasiB0BNM1.
PRIDEiB0BNM1.

PTM databases

iPTMnetiB0BNM1.
PhosphoSitePlusiB0BNM1.

Expressioni

Gene expression databases

BgeeiENSRNOG00000019201.
GenevisibleiB0BNM1. RN.

Interactioni

Subunit structurei

Homodimer. Interacts with APOA1 and APOA2.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025986.

Structurei

3D structure databases

ProteinModelPortaliB0BNM1.
SMRiB0BNM1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 269YjeF N-terminalUniRule annotationAdd BLAST211

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni113 – 117NAD(P)HXUniRule annotation5
Regioni183 – 189NAD(P)HXUniRule annotation7

Sequence similaritiesi

Belongs to the NnrE/AIBP family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2585. Eukaryota.
COG0062. LUCA.
GeneTreeiENSGT00390000007227.
HOGENOMiHOG000174236.
HOVERGENiHBG058276.
InParanoidiB0BNM1.
OMAiATQFTGR.
OrthoDBiEOG091G0J79.
PhylomeDBiB0BNM1.
TreeFamiTF300197.

Family and domain databases

Gene3Di3.40.50.10260. 1 hit.
HAMAPiMF_01966. NADHX_epimerase. 1 hit.
InterProiView protein in InterPro
IPR004443. YjeF_N_dom.
IPR032976. YJEFN_prot_eukaryotes.
PANTHERiPTHR13232. PTHR13232. 1 hit.
PfamiView protein in Pfam
PF03853. YjeF_N. 1 hit.
SUPFAMiSSF64153. SSF64153. 1 hit.
TIGRFAMsiTIGR00197. yjeF_nterm. 1 hit.
PROSITEiView protein in PROSITE
PS51385. YJEF_N. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0BNM1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGLRTLLGL GLLVAGSRLP RIASRQSVCR AGPIWWGTQH RSSETMASAA
60 70 80 90 100
VKYLSQEEAQ AVDEELFNEY QFSVDQLMEL AGLSCATAIA KAYPPTSMSK
110 120 130 140 150
SPPTVLVICG PGNNGGDGLV CARHLKLFGY QPTIYYPKRP NKPLFTGLVT
160 170 180 190 200
QCQKMDIPFL GEMPPEPMMV DELYELVVDA IFGFSFKGDV REPFHSILSV
210 220 230 240 250
LSGLTVPIAS IDIPSGWDVE KGNPSGIQPD LLISLTAPKK SATQFTGRYH
260 270 280
YLGGRFVPPA LEKKYQLNLP AYPDTECVYR LQ
Length:282
Mass (Da):30,891
Last modified:February 26, 2008 - v1
Checksum:iC879808921955A18
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473976 Genomic DNA. Translation: EDM00740.1.
BC158876 mRNA. Translation: AAI58877.1.
RefSeqiXP_006232671.1. XM_006232609.2.
UniGeneiRn.14886.

Genome annotation databases

EnsembliENSRNOT00000025986; ENSRNOP00000025986; ENSRNOG00000019201.
GeneIDi295229.

Similar proteinsi

Entry informationi

Entry nameiNNRE_RAT
AccessioniPrimary (citable) accession number: B0BNM1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: February 26, 2008
Last modified: August 30, 2017
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families