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Protein

NAD(P)H-hydrate epimerase

Gene

Naxe

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.UniRule annotation

Catalytic activityi

(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi114 – 1141PotassiumUniRule annotation
Metal bindingi179 – 1791PotassiumUniRule annotation
Binding sitei212 – 2121NAD(P)HXUniRule annotation
Metal bindingi215 – 2151PotassiumUniRule annotation

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • NADHX epimerase activity Source: MGI
  • NADPHX epimerase activity Source: MGI
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Enzyme and pathway databases

BRENDAi5.1.99.6. 5301.
ReactomeiR-RNO-197264. Nicotinamide salvaging.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H-hydrate epimeraseUniRule annotation (EC:5.1.99.6)
Alternative name(s):
Apolipoprotein A-I-binding proteinUniRule annotation
Short name:
AI-BPUniRule annotation
NAD(P)HX epimeraseImported
Gene namesi
Name:NaxeImported
Synonyms:Aibp, Apoa1bp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1304699. Naxe.

Subcellular locationi

  • Mitochondrion UniRule annotation
  • Secreted UniRule annotation

  • Note: In sperm, secretion gradually increases during capacitation.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5353MitochondrionUniRule annotationAdd
BLAST
Chaini54 – 282229NAD(P)H-hydrate epimerasePRO_0000416310Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei138 – 1381N6-succinyllysineBy similarity

Post-translational modificationi

Undergoes physiological phosphorylation during sperm capacitation, downstream to PKA activation.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiB0BNM1.
PeptideAtlasiB0BNM1.
PRIDEiB0BNM1.

PTM databases

iPTMnetiB0BNM1.
PhosphoSiteiB0BNM1.

Expressioni

Gene expression databases

BgeeiENSRNOG00000019201.
GenevisibleiB0BNM1. RN.

Interactioni

Subunit structurei

Homodimer. Interacts with APOA1 and APOA2.UniRule annotation

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025986.

Structurei

3D structure databases

ProteinModelPortaliB0BNM1.
SMRiB0BNM1. Positions 50-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini59 – 269211YjeF N-terminalUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni113 – 1175NAD(P)HXUniRule annotation
Regioni183 – 1897NAD(P)HXUniRule annotation

Sequence similaritiesi

Belongs to the NnrE/AIBP family.UniRule annotation
Contains 1 YjeF N-terminal domain.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2585. Eukaryota.
COG0062. LUCA.
GeneTreeiENSGT00390000007227.
HOGENOMiHOG000174236.
HOVERGENiHBG058276.
InParanoidiB0BNM1.
OMAiHLGMFGY.
OrthoDBiEOG091G0J79.
PhylomeDBiB0BNM1.
TreeFamiTF300197.

Family and domain databases

Gene3Di3.40.50.10260. 1 hit.
HAMAPiMF_01966. NADHX_epimerase. 1 hit.
InterProiIPR004443. YjeF_N_dom.
IPR032976. YJEFN_prot_eukaryotes.
[Graphical view]
PANTHERiPTHR13232. PTHR13232. 1 hit.
PfamiPF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMiSSF64153. SSF64153. 1 hit.
TIGRFAMsiTIGR00197. yjeF_nterm. 1 hit.
PROSITEiPS51385. YJEF_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0BNM1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGLRTLLGL GLLVAGSRLP RIASRQSVCR AGPIWWGTQH RSSETMASAA
60 70 80 90 100
VKYLSQEEAQ AVDEELFNEY QFSVDQLMEL AGLSCATAIA KAYPPTSMSK
110 120 130 140 150
SPPTVLVICG PGNNGGDGLV CARHLKLFGY QPTIYYPKRP NKPLFTGLVT
160 170 180 190 200
QCQKMDIPFL GEMPPEPMMV DELYELVVDA IFGFSFKGDV REPFHSILSV
210 220 230 240 250
LSGLTVPIAS IDIPSGWDVE KGNPSGIQPD LLISLTAPKK SATQFTGRYH
260 270 280
YLGGRFVPPA LEKKYQLNLP AYPDTECVYR LQ
Length:282
Mass (Da):30,891
Last modified:February 26, 2008 - v1
Checksum:iC879808921955A18
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473976 Genomic DNA. Translation: EDM00740.1.
BC158876 mRNA. Translation: AAI58877.1.
RefSeqiXP_006232671.1. XM_006232609.2.
UniGeneiRn.14886.

Genome annotation databases

EnsembliENSRNOT00000025986; ENSRNOP00000025986; ENSRNOG00000019201.
GeneIDi295229.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473976 Genomic DNA. Translation: EDM00740.1.
BC158876 mRNA. Translation: AAI58877.1.
RefSeqiXP_006232671.1. XM_006232609.2.
UniGeneiRn.14886.

3D structure databases

ProteinModelPortaliB0BNM1.
SMRiB0BNM1. Positions 50-282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025986.

PTM databases

iPTMnetiB0BNM1.
PhosphoSiteiB0BNM1.

Proteomic databases

PaxDbiB0BNM1.
PeptideAtlasiB0BNM1.
PRIDEiB0BNM1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025986; ENSRNOP00000025986; ENSRNOG00000019201.
GeneIDi295229.

Organism-specific databases

CTDi128240.
RGDi1304699. Naxe.

Phylogenomic databases

eggNOGiKOG2585. Eukaryota.
COG0062. LUCA.
GeneTreeiENSGT00390000007227.
HOGENOMiHOG000174236.
HOVERGENiHBG058276.
InParanoidiB0BNM1.
OMAiHLGMFGY.
OrthoDBiEOG091G0J79.
PhylomeDBiB0BNM1.
TreeFamiTF300197.

Enzyme and pathway databases

BRENDAi5.1.99.6. 5301.
ReactomeiR-RNO-197264. Nicotinamide salvaging.

Miscellaneous databases

PROiB0BNM1.

Gene expression databases

BgeeiENSRNOG00000019201.
GenevisibleiB0BNM1. RN.

Family and domain databases

Gene3Di3.40.50.10260. 1 hit.
HAMAPiMF_01966. NADHX_epimerase. 1 hit.
InterProiIPR004443. YjeF_N_dom.
IPR032976. YJEFN_prot_eukaryotes.
[Graphical view]
PANTHERiPTHR13232. PTHR13232. 1 hit.
PfamiPF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMiSSF64153. SSF64153. 1 hit.
TIGRFAMsiTIGR00197. yjeF_nterm. 1 hit.
PROSITEiPS51385. YJEF_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNNRE_RAT
AccessioniPrimary (citable) accession number: B0BNM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: February 26, 2008
Last modified: September 7, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.