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Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC1

Gene

Polr2e

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription

Enzyme and pathway databases

ReactomeiR-RNO-112382. Formation of RNA Pol II elongation complex.
R-RNO-113418. Formation of the Early Elongation Complex.
R-RNO-1834949. Cytosolic sensors of pathogen-associated DNA.
R-RNO-5250924. B-WICH complex positively regulates rRNA expression.
R-RNO-5578749. Transcriptional regulation by small RNAs.
R-RNO-674695. RNA Polymerase II Pre-transcription Events.
R-RNO-6781823. Formation of TC-NER Pre-Incision Complex.
R-RNO-6782135. Dual incision in TC-NER.
R-RNO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-RNO-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-RNO-6803529. FGFR2 alternative splicing.
R-RNO-6807505. RNA polymerase II transcribes snRNA genes.
R-RNO-72086. mRNA Capping.
R-RNO-72163. mRNA Splicing - Major Pathway.
R-RNO-72165. mRNA Splicing - Minor Pathway.
R-RNO-72203. Processing of Capped Intron-Containing Pre-mRNA.
R-RNO-73762. RNA Polymerase I Transcription Initiation.
R-RNO-73772. RNA Polymerase I Promoter Escape.
R-RNO-73776. RNA Polymerase II Promoter Escape.
R-RNO-73777. RNA Polymerase I Chain Elongation.
R-RNO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-RNO-73863. RNA Polymerase I Transcription Termination.
R-RNO-75953. RNA Polymerase II Transcription Initiation.
R-RNO-75955. RNA Polymerase II Transcription Elongation.
R-RNO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-RNO-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-RNO-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-RNO-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
R-RNO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC1
Short name:
RNA polymerases I, II, and III subunit ABC1
Alternative name(s):
DNA-directed RNA polymerase II subunit E
RPB5 homolog
Gene namesi
Name:Polr2e
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1589817. Polr2e.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210DNA-directed RNA polymerases I, II, and III subunit RPABC1PRO_0000329305Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiB0BNE2.
PRIDEiB0BNE2.

Expressioni

Gene expression databases

GenevisibleiB0BNE2. RN.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively. In RNA Pol II, this subunit is present in 2-fold molar excess over the other subunits. Interacts with URI1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi605776. 1 interaction.
STRINGi10116.ENSRNOP00000018169.

Structurei

3D structure databases

ProteinModelPortaliB0BNE2.
SMRiB0BNE2. Positions 3-209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3218. Eukaryota.
COG2012. LUCA.
GeneTreeiENSGT00390000013841.
HOGENOMiHOG000205213.
HOVERGENiHBG057019.
InParanoidiB0BNE2.
KOiK03013.
OMAiNQLMRIQ.
OrthoDBiEOG7M6D8D.
PhylomeDBiB0BNE2.
TreeFamiTF103040.

Family and domain databases

Gene3Di3.40.1340.10. 1 hit.
3.90.940.20. 1 hit.
HAMAPiMF_00025. RNApol_RpoH_RPB5.
InterProiIPR014381. DNA_RNA_pol_RPB5_euk/virus.
IPR005571. RNA_pol_Rpb5_N.
IPR000783. RNA_pol_subH/Rpb5_C.
IPR020608. RNA_pol_subH/Rpb5_CS.
IPR020609. RpoH/RPB5.
[Graphical view]
PfamiPF01191. RNA_pol_Rpb5_C. 1 hit.
PF03871. RNA_pol_Rpb5_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000747. RPB5. 1 hit.
ProDomiPD005155. RNA_pol_subH/Rpb5_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53036. SSF53036. 1 hit.
SSF55287. SSF55287. 1 hit.
PROSITEiPS01110. RNA_POL_H_23KD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0BNE2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQFGDKPSE
60 70 80 90 100
GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKVY CQRMQEENIT
110 120 130 140 150
RALIVVQQGM TPSAKQSLVD MAPKYVLEQF LQQELLINIT EHELVPEHVV
160 170 180 190 200
MTKEEVTELL ARYKLRESQL PRIQAGDPVA RYFGIKRGQV VKIIRPSETA
210
GRYITYRLVQ
Length:210
Mass (Da):24,570
Last modified:February 26, 2008 - v1
Checksum:iE09DEB40E05F3554
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC158787 mRNA. Translation: AAI58788.1.
RefSeqiNP_001103084.1. NM_001109614.1.
UniGeneiRn.65038.

Genome annotation databases

EnsembliENSRNOT00000018169; ENSRNOP00000018169; ENSRNOG00000013545.
GeneIDi690966.
KEGGirno:690966.
UCSCiRGD:1589817. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC158787 mRNA. Translation: AAI58788.1.
RefSeqiNP_001103084.1. NM_001109614.1.
UniGeneiRn.65038.

3D structure databases

ProteinModelPortaliB0BNE2.
SMRiB0BNE2. Positions 3-209.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi605776. 1 interaction.
STRINGi10116.ENSRNOP00000018169.

Proteomic databases

PaxDbiB0BNE2.
PRIDEiB0BNE2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018169; ENSRNOP00000018169; ENSRNOG00000013545.
GeneIDi690966.
KEGGirno:690966.
UCSCiRGD:1589817. rat.

Organism-specific databases

CTDi5434.
RGDi1589817. Polr2e.

Phylogenomic databases

eggNOGiKOG3218. Eukaryota.
COG2012. LUCA.
GeneTreeiENSGT00390000013841.
HOGENOMiHOG000205213.
HOVERGENiHBG057019.
InParanoidiB0BNE2.
KOiK03013.
OMAiNQLMRIQ.
OrthoDBiEOG7M6D8D.
PhylomeDBiB0BNE2.
TreeFamiTF103040.

Enzyme and pathway databases

ReactomeiR-RNO-112382. Formation of RNA Pol II elongation complex.
R-RNO-113418. Formation of the Early Elongation Complex.
R-RNO-1834949. Cytosolic sensors of pathogen-associated DNA.
R-RNO-5250924. B-WICH complex positively regulates rRNA expression.
R-RNO-5578749. Transcriptional regulation by small RNAs.
R-RNO-674695. RNA Polymerase II Pre-transcription Events.
R-RNO-6781823. Formation of TC-NER Pre-Incision Complex.
R-RNO-6782135. Dual incision in TC-NER.
R-RNO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-RNO-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-RNO-6803529. FGFR2 alternative splicing.
R-RNO-6807505. RNA polymerase II transcribes snRNA genes.
R-RNO-72086. mRNA Capping.
R-RNO-72163. mRNA Splicing - Major Pathway.
R-RNO-72165. mRNA Splicing - Minor Pathway.
R-RNO-72203. Processing of Capped Intron-Containing Pre-mRNA.
R-RNO-73762. RNA Polymerase I Transcription Initiation.
R-RNO-73772. RNA Polymerase I Promoter Escape.
R-RNO-73776. RNA Polymerase II Promoter Escape.
R-RNO-73777. RNA Polymerase I Chain Elongation.
R-RNO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-RNO-73863. RNA Polymerase I Transcription Termination.
R-RNO-75953. RNA Polymerase II Transcription Initiation.
R-RNO-75955. RNA Polymerase II Transcription Elongation.
R-RNO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-RNO-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-RNO-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-RNO-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
R-RNO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

PROiB0BNE2.

Gene expression databases

GenevisibleiB0BNE2. RN.

Family and domain databases

Gene3Di3.40.1340.10. 1 hit.
3.90.940.20. 1 hit.
HAMAPiMF_00025. RNApol_RpoH_RPB5.
InterProiIPR014381. DNA_RNA_pol_RPB5_euk/virus.
IPR005571. RNA_pol_Rpb5_N.
IPR000783. RNA_pol_subH/Rpb5_C.
IPR020608. RNA_pol_subH/Rpb5_CS.
IPR020609. RpoH/RPB5.
[Graphical view]
PfamiPF01191. RNA_pol_Rpb5_C. 1 hit.
PF03871. RNA_pol_Rpb5_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000747. RPB5. 1 hit.
ProDomiPD005155. RNA_pol_subH/Rpb5_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53036. SSF53036. 1 hit.
SSF55287. SSF55287. 1 hit.
PROSITEiPS01110. RNA_POL_H_23KD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiRPAB1_RAT
AccessioniPrimary (citable) accession number: B0BNE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: February 26, 2008
Last modified: June 8, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.