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Protein

Ectonucleotide pyrophosphatase/phosphodiesterase family member 6

Gene

Enpp6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Choline-specific glycerophosphodiester phosphodiesterase. The preferred substrate may be lysosphingomyelin (By similarity). Hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. Hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine in vitro, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate. Does not hydrolyze diacyl phospholipids such as phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol and phosphatidic acid (By similarity).By similarity

Catalytic activityi

A lysophosphatidylcholine + H2O = a monoacylglycerol + phosphocholine.
sn-glycero-3-phosphocholine + H2O = glycerol + phosphocholine.

Cofactori

Enzyme regulationi

Inhibited by EDTA and EGTA in vitro.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711NucleophileSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-RNO-6814848. Glycerophospholipid catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 6 (EC:3.1.4.-, EC:3.1.4.38)
Short name:
E-NPP 6
Short name:
NPP-6
Alternative name(s):
Choline-specific glycerophosphodiester phosphodiesterase
Glycerophosphocholine cholinephosphodiesterase
Short name:
GPC-Cpde
Gene namesi
Name:Enpp6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi1311645. Enpp6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 419397Ectonucleotide pyrophosphatase/phosphodiesterase family member 6PRO_0000366924Add
BLAST
Propeptidei420 – 44021Removed in mature formSequence analysisPRO_0000420894Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei71 – 711PhosphoserineCombined sources
Glycosylationi100 – 1001N-linked (GlcNAc...)By similarity
Glycosylationi118 – 1181N-linked (GlcNAc...)By similarity
Disulfide bondi142 ↔ 154By similarity
Glycosylationi341 – 3411N-linked (GlcNAc...)By similarity
Glycosylationi404 – 4041N-linked (GlcNAc...)By similarity
Disulfide bondi412 – 412InterchainBy similarity
Lipidationi419 – 4191GPI-anchor amidated serineSequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiB0BND0.
PRIDEiB0BND0.

PTM databases

iPTMnetiB0BND0.
PhosphoSiteiB0BND0.
UniCarbKBiB0BND0.

Expressioni

Gene expression databases

BgeeiENSRNOG00000009660.
ExpressionAtlasiB0BND0. baseline and differential.
GenevisibleiB0BND0. RN.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013005.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2645. Eukaryota.
COG1524. LUCA.
GeneTreeiENSGT00760000119157.
HOGENOMiHOG000112376.
HOVERGENiHBG107851.
InParanoidiB0BND0.
OMAiCNVVGIT.
OrthoDBiEOG091G06NA.
PhylomeDBiB0BND0.
TreeFamiTF330032.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0BND0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKLWTFLL LFGFSWVWPA SAHRKLLVLL LDGFRSDYIS EDALASLPGF
60 70 80 90 100
REIVNRGVKV DYLTPDFPSL SYPNYYTLMT GRHCEVHQMI GNYMWDPRTN
110 120 130 140 150
KSFDIGVNRD SLMPLWWNGS EPLWITLMKA RRKVYMYYWP GCEVEILGVR
160 170 180 190 200
PTYCLEYKNV PTDINFANAV SDALDSLKSG RADLAAIYHE RIDVEGHHYG
210 220 230 240 250
PSSPQRKDAL KAVDTVLKYM TQWIQERGLQ NDLNVILFSD HGMTDIFWMD
260 270 280 290 300
KVIELSKYIS LDDLQQVKDQ GPVVSLWPVP EKHSEIYHKL RTVEHMTVYE
310 320 330 340 350
KEAIPNRFYY KKGKFVSPLT LVADEGWFIA ESREALPFWM NSTGKREGWQ
360 370 380 390 400
HGWHGYDNEL MDMRGIFLAF GPDFKSNFRA APIRSVDVYN IMCNVVGITP
410 420 430 440
LPNNGSWSRV VCMLKSQTSS SPSIPPNSCA LVLILLLYFV
Length:440
Mass (Da):50,701
Last modified:February 26, 2008 - v1
Checksum:i4A76DB3E3EF8AD08
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC158772 mRNA. Translation: AAI58773.1.
RefSeqiXP_006253218.1. XM_006253156.2.
UniGeneiRn.8484.

Genome annotation databases

EnsembliENSRNOT00000013005; ENSRNOP00000013005; ENSRNOG00000009660.
GeneIDi306460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC158772 mRNA. Translation: AAI58773.1.
RefSeqiXP_006253218.1. XM_006253156.2.
UniGeneiRn.8484.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013005.

PTM databases

iPTMnetiB0BND0.
PhosphoSiteiB0BND0.
UniCarbKBiB0BND0.

Proteomic databases

PaxDbiB0BND0.
PRIDEiB0BND0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013005; ENSRNOP00000013005; ENSRNOG00000009660.
GeneIDi306460.

Organism-specific databases

CTDi133121.
RGDi1311645. Enpp6.

Phylogenomic databases

eggNOGiKOG2645. Eukaryota.
COG1524. LUCA.
GeneTreeiENSGT00760000119157.
HOGENOMiHOG000112376.
HOVERGENiHBG107851.
InParanoidiB0BND0.
OMAiCNVVGIT.
OrthoDBiEOG091G06NA.
PhylomeDBiB0BND0.
TreeFamiTF330032.

Enzyme and pathway databases

ReactomeiR-RNO-6814848. Glycerophospholipid catabolism.

Miscellaneous databases

PROiB0BND0.

Gene expression databases

BgeeiENSRNOG00000009660.
ExpressionAtlasiB0BND0. baseline and differential.
GenevisibleiB0BND0. RN.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENPP6_RAT
AccessioniPrimary (citable) accession number: B0BND0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: February 26, 2008
Last modified: September 7, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.