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Protein

Enolase 4

Gene

eno4

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.By similarity
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gapdh), Glyceraldehyde-3-phosphate dehydrogenase (gapdh)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase 4 (eno4)
  5. Pyruvate kinase (pkm), Pyruvate kinase (XENTR_v90027607mg), Pyruvate kinase (pklr), Pyruvate kinase (pkm), Pyruvate kinase (pkm)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei287SubstrateBy similarity1
Active sitei467Proton acceptorBy similarity1
Binding sitei518SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase 4 (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
Gene namesi
Name:eno4
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
Proteomesi
  • UP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-1009720. eno4.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003484581 – 574Enolase 4Add BLAST574

Structurei

3D structure databases

ProteinModelPortaliB0BM20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

HOVERGENiHBG107850.
InParanoidiB0BM20.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 2 hits.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 2 hits.

Sequencei

Sequence statusi: Complete.

B0BM20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYRAGDAAR RARERYELKQ AAAEFYRKLS VTERLEEALN STFCLGPEDV
60 70 80 90 100
YGHLANYFAQ FSKPPTICQI RGRKVLDGTG EPTVEAEVFC TVKNMDKRIC
110 120 130 140 150
SSVISAASEH PKASKGPEQE SNHSADIAIQ WLNDLSPKLR GMSPDEQNKI
160 170 180 190 200
DQLLSDFYQP KIEEEKERRQ MEREASPMPL QPEPSPVTSP APGKKKGSGK
210 220 230 240 250
GKKAAVVEKP IPPEETPEAV VPGSPAIGAL SLAVAKASSV LSKTPLYLHI
260 270 280 290 300
RALRNEKLPT EFFMPTPMIS ILSCGTSSPG KLNLMKEVLI IPQTGLTVQQ
310 320 330 340 350
SLDMALMLQN QIVKQINAAS KTGPAIKNVS PLGCMLIGGD RIEQPLDLIC
360 370 380 390 400
EACQHVGLEL GTNLYLAINC AAHELMDYNK GKYEVLSGTF KSPDEMIDLY
410 420 430 440 450
VDLINRQPAI LALLDPLRKE DTVQWESLAK ALGSKCYLFA DAASKPVCKL
460 470 480 490 500
LESGSMNSPP CSGTVIKHTN EITISQLLGV FKLIEGENRV AVLGCPYKES
510 520 530 540 550
VGDSTADLAV GLGARFVKLG GLLRGERTTK YNRLLAIEDE LTQAGALGFW
560 570
TKNEFPVLCE VQNQPGPQET PETQ
Length:574
Mass (Da):62,651
Last modified:February 26, 2008 - v1
Checksum:i67D536E7B27C74C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC158253 mRNA. Translation: AAI58254.1.
RefSeqiNP_001119992.1. NM_001126520.1.
UniGeneiStr.28845.

Genome annotation databases

GeneIDi100144948.
KEGGixtr:100144948.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC158253 mRNA. Translation: AAI58254.1.
RefSeqiNP_001119992.1. NM_001126520.1.
UniGeneiStr.28845.

3D structure databases

ProteinModelPortaliB0BM20.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100144948.
KEGGixtr:100144948.

Organism-specific databases

CTDi387712.
XenbaseiXB-GENE-1009720. eno4.

Phylogenomic databases

HOVERGENiHBG107850.
InParanoidiB0BM20.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 2 hits.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiENO4_XENTR
AccessioniPrimary (citable) accession number: B0BM20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: February 26, 2008
Last modified: February 15, 2017
This is version 39 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Although it belongs to the enolase family, Leu-332 is present instead of the conserved Glu which is expected to be an active site residue.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.