Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leucine-rich repeat-containing G-protein coupled receptor 4

Gene

lgr4

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye. May play a role in regulating the circadian rhythms of plasma lipids (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Biological rhythms, Differentiation, Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat-containing G-protein coupled receptor 4
Gene namesi
Name:lgr4
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
Proteomesi
  • UP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-5959108. lgr4.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 547ExtracellularSequence analysisAdd BLAST526
Transmembranei548 – 568Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini569 – 578CytoplasmicSequence analysis10
Transmembranei579 – 599Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini600 – 623ExtracellularSequence analysisAdd BLAST24
Transmembranei624 – 644Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini645 – 666CytoplasmicSequence analysisAdd BLAST22
Transmembranei667 – 687Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini688 – 706ExtracellularSequence analysisAdd BLAST19
Transmembranei707 – 727Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini728 – 759CytoplasmicSequence analysisAdd BLAST32
Transmembranei760 – 780Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini781 – 786ExtracellularSequence analysis6
Transmembranei787 – 807Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini808 – 955CytoplasmicSequence analysisAdd BLAST148

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000042281622 – 955Leucine-rich repeat-containing G-protein coupled receptor 4Add BLAST934

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 37PROSITE-ProRule annotation
Disulfide bondi35 ↔ 45PROSITE-ProRule annotation
Glycosylationi201N-linked (GlcNAc...)Sequence analysis1
Glycosylationi296N-linked (GlcNAc...)Sequence analysis1
Glycosylationi316N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi341 ↔ 366PROSITE-ProRule annotation
Glycosylationi384N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi472 ↔ 525PROSITE-ProRule annotation
Disulfide bondi473 ↔ 478PROSITE-ProRule annotation
Disulfide bondi621 ↔ 696PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiB0BLW3.

Expressioni

Developmental stagei

Maternally and zygotically expressed. Zygotic expression increases after gastrula stage. Expressed in all three germ layers.1 Publication

Interactioni

Protein-protein interaction databases

DIPiDIP-60560N.
IntActiB0BLW3. 1 interactor.
MINTiMINT-8297617.
STRINGi8364.ENSXETP00000061102.

Structurei

Secondary structure

1955
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 37Combined sources5
Beta strandi39 – 44Combined sources6
Beta strandi62 – 65Combined sources4
Turni76 – 81Combined sources6
Beta strandi87 – 89Combined sources3
Turni100 – 105Combined sources6
Beta strandi111 – 113Combined sources3
Turni124 – 129Combined sources6
Beta strandi135 – 137Combined sources3
Turni148 – 153Combined sources6
Beta strandi159 – 161Combined sources3
Helixi172 – 175Combined sources4
Beta strandi183 – 185Combined sources3
Turni196 – 201Combined sources6
Beta strandi207 – 209Combined sources3
Turni220 – 225Combined sources6
Beta strandi231 – 233Combined sources3
Helixi244 – 248Combined sources5
Beta strandi254 – 256Combined sources3
Turni267 – 272Combined sources6
Beta strandi278 – 280Combined sources3
Turni291 – 294Combined sources4
Beta strandi302 – 306Combined sources5
Beta strandi324 – 331Combined sources8
Helixi339 – 343Combined sources5
Beta strandi349 – 351Combined sources3
Beta strandi371 – 373Combined sources3
Beta strandi384 – 387Combined sources4
Beta strandi395 – 397Combined sources3
Helixi407 – 409Combined sources3
Beta strandi419 – 421Combined sources3
Beta strandi424 – 426Combined sources3
Beta strandi439 – 442Combined sources4
Helixi450 – 452Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LI1X-ray2.66A/B23-454[»]
4LI2X-ray3.19A23-454[»]
ProteinModelPortaliB0BLW3.
SMRiB0BLW3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 59LRRNTAdd BLAST34
Repeati57 – 81LRR 1Add BLAST25
Repeati83 – 105LRR 2Add BLAST23
Repeati106 – 129LRR 3Add BLAST24
Repeati131 – 153LRR 4Add BLAST23
Repeati155 – 177LRR 5Add BLAST23
Repeati178 – 201LRR 6Add BLAST24
Repeati203 – 225LRR 7Add BLAST23
Repeati226 – 249LRR 8Add BLAST24
Repeati250 – 272LRR 9Add BLAST23
Repeati274 – 296LRR 10Add BLAST23
Repeati320 – 343LRR 11Add BLAST24
Repeati345 – 365LRR 12Add BLAST21
Repeati366 – 389LRR 13Add BLAST24
Repeati390 – 413LRR 14Add BLAST24
Repeati415 – 437LRR 15Add BLAST23

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Contains 15 LRR (leucine-rich) repeats.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
KOG2087. Eukaryota.
COG4886. LUCA.
HOVERGENiHBG031675.
KOiK04309.
TreeFamiTF316814.

Family and domain databases

Gene3Di3.80.10.10. 3 hits.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002131. Gphrmn_rcpt_fam.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
PF13855. LRR_8. 4 hits.
[Graphical view]
PRINTSiPR00373. GLYCHORMONER.
PR00237. GPCRRHODOPSN.
SMARTiSM00369. LRR_TYP. 14 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS51450. LRR. 15 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0BLW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCPGWPLAL FALLLASCSG GPSGVSSPAP CPAPCACDLD GGADCSGKGL
60 70 80 90 100
VTVPDGLSVF THSLDLSMNN ITKLPEGAFK GFPYLEELRL AGNDLSIIHP
110 120 130 140 150
MALSGLKELK VLTLQNNQLK TVPSESLKGL VSLQSLRLDA NHIVTVPEDS
160 170 180 190 200
FEGLVQLRHL WLDDNSLTEV PIRPLSNLPS LQALTLALNK ISHIPDYAFS
210 220 230 240 250
NLSSLVVLHL HNNKIRTLGP HCFHGLDNLE ALDLNYNNLI DFPDSIRSLP
260 270 280 290 300
NLKELGFHSN SITIIPDGAF VKNPLLRTIH LYDNPLSFVG NSAFQNLSDL
310 320 330 340 350
HFLIIRGASN VQWFPNLTGT NNLESLTLTG TKIRSIPIKF CQEQKMLRTL
360 370 380 390 400
DLSYNEISAL VGFEGCSSLE EVYLQNNQIQ EVQNETFQGL AALRMLDLSR
410 420 430 440 450
NRIHTIHKEA FVTLKALTNL DLSFNDLTAF PTAGLHGLNQ LKLTGNPNFK
460 470 480 490 500
ETLTAKDLIK LSSVSVPYAY QCCAFSACNS YMTTTVEEDR LRAQRLLLDH
510 520 530 540 550
DRAAMDPDYM GTEDDKEHVQ ALIQCNPATG PFKPCEYLLG SWMIRLTVWF
560 570 580 590 600
IFLLALIFNV IVIVTMFASC SQLTSSKLFI GLIAVSNLFM GVYTGTLTVL
610 620 630 640 650
DTISWGQFAE FGIWWETGNG CKVAGFLAIF SSESAIFFLM LAAIERSLSA
660 670 680 690 700
KDIIKKEKHQ HLRKFQVASL LAVLLAAAAG CLPLFHIGEF SSSPLCLPFP
710 720 730 740 750
TGETPSLGFT VTLVLLNSLA FLIMVITYTK LYCTIEKEDL SENAESSMIK
760 770 780 790 800
HVAWLIFTNC IFFCPVAFFS FAPLITAIYI SPEIMKSVTL IFLPLPACLN
810 820 830 840 850
PVLYVFFNPK FKEDWKLLRW RLTKRSGSVA VATNSQRGCV TQDFYYDFGM
860 870 880 890 900
YSHLQGGNFA VCDYCESVLL KNPPPCKHLI KSHSCPTLAV VPCQRPDNYW
910 920 930 940 950
SEFGTQSAHS DCADEEDSFV SDSSDQVQVC GRACFYQSRG LPLVRYAYNI

PRMKD
Length:955
Mass (Da):105,725
Last modified:February 26, 2008 - v1
Checksum:iE36B9BD93557CCA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAMC01072689 Genomic DNA. No translation available.
AAMC01072690 Genomic DNA. No translation available.
AAMC01072691 Genomic DNA. No translation available.
AAMC01072692 Genomic DNA. No translation available.
AAMC01072693 Genomic DNA. No translation available.
AAMC01072694 Genomic DNA. No translation available.
AAMC01072695 Genomic DNA. No translation available.
AAMC01072696 Genomic DNA. No translation available.
BC158183 mRNA. Translation: AAI58184.1.
RefSeqiNP_001119970.1. NM_001126498.1.
UniGeneiStr.14297.

Genome annotation databases

GeneIDi100144922.
KEGGixtr:100144922.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAMC01072689 Genomic DNA. No translation available.
AAMC01072690 Genomic DNA. No translation available.
AAMC01072691 Genomic DNA. No translation available.
AAMC01072692 Genomic DNA. No translation available.
AAMC01072693 Genomic DNA. No translation available.
AAMC01072694 Genomic DNA. No translation available.
AAMC01072695 Genomic DNA. No translation available.
AAMC01072696 Genomic DNA. No translation available.
BC158183 mRNA. Translation: AAI58184.1.
RefSeqiNP_001119970.1. NM_001126498.1.
UniGeneiStr.14297.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LI1X-ray2.66A/B23-454[»]
4LI2X-ray3.19A23-454[»]
ProteinModelPortaliB0BLW3.
SMRiB0BLW3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60560N.
IntActiB0BLW3. 1 interactor.
MINTiMINT-8297617.
STRINGi8364.ENSXETP00000061102.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiB0BLW3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100144922.
KEGGixtr:100144922.

Organism-specific databases

CTDi55366.
XenbaseiXB-GENE-5959108. lgr4.

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
KOG2087. Eukaryota.
COG4886. LUCA.
HOVERGENiHBG031675.
KOiK04309.
TreeFamiTF316814.

Family and domain databases

Gene3Di3.80.10.10. 3 hits.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002131. Gphrmn_rcpt_fam.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
PF13855. LRR_8. 4 hits.
[Graphical view]
PRINTSiPR00373. GLYCHORMONER.
PR00237. GPCRRHODOPSN.
SMARTiSM00369. LRR_TYP. 14 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS51450. LRR. 15 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLGR4_XENTR
AccessioniPrimary (citable) accession number: B0BLW3
Secondary accession number(s): F6US71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: February 26, 2008
Last modified: November 2, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.