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Protein

Leucine-rich repeat-containing G-protein coupled receptor 4

Gene

lgr4

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye. May play a role in regulating the circadian rhythms of plasma lipids (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Biological rhythms, Differentiation, Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat-containing G-protein coupled receptor 4
Gene namesi
Name:lgr4
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
Proteomesi
  • UP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-5959108. lgr4.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 547526ExtracellularSequence analysisAdd
BLAST
Transmembranei548 – 56821Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini569 – 57810CytoplasmicSequence analysis
Transmembranei579 – 59921Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini600 – 62324ExtracellularSequence analysisAdd
BLAST
Transmembranei624 – 64421Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini645 – 66622CytoplasmicSequence analysisAdd
BLAST
Transmembranei667 – 68721Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini688 – 70619ExtracellularSequence analysisAdd
BLAST
Transmembranei707 – 72721Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini728 – 75932CytoplasmicSequence analysisAdd
BLAST
Transmembranei760 – 78021Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini781 – 7866ExtracellularSequence analysis
Transmembranei787 – 80721Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini808 – 955148CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 955934Leucine-rich repeat-containing G-protein coupled receptor 4PRO_0000422816Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 37PROSITE-ProRule annotation
Disulfide bondi35 ↔ 45PROSITE-ProRule annotation
Glycosylationi201 – 2011N-linked (GlcNAc...)Sequence analysis
Glycosylationi296 – 2961N-linked (GlcNAc...)Sequence analysis
Glycosylationi316 – 3161N-linked (GlcNAc...)Sequence analysis
Disulfide bondi341 ↔ 366PROSITE-ProRule annotation
Glycosylationi384 – 3841N-linked (GlcNAc...)Sequence analysis
Disulfide bondi472 ↔ 525PROSITE-ProRule annotation
Disulfide bondi473 ↔ 478PROSITE-ProRule annotation
Disulfide bondi621 ↔ 696PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiB0BLW3.

Expressioni

Developmental stagei

Maternally and zygotically expressed. Zygotic expression increases after gastrula stage. Expressed in all three germ layers.1 Publication

Interactioni

Protein-protein interaction databases

DIPiDIP-60560N.
IntActiB0BLW3. 1 interaction.
MINTiMINT-8297617.
STRINGi8364.ENSXETP00000061102.

Structurei

Secondary structure

1
955
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi33 – 375Combined sources
Beta strandi39 – 446Combined sources
Beta strandi62 – 654Combined sources
Turni76 – 816Combined sources
Beta strandi87 – 893Combined sources
Turni100 – 1056Combined sources
Beta strandi111 – 1133Combined sources
Turni124 – 1296Combined sources
Beta strandi135 – 1373Combined sources
Turni148 – 1536Combined sources
Beta strandi159 – 1613Combined sources
Helixi172 – 1754Combined sources
Beta strandi183 – 1853Combined sources
Turni196 – 2016Combined sources
Beta strandi207 – 2093Combined sources
Turni220 – 2256Combined sources
Beta strandi231 – 2333Combined sources
Helixi244 – 2485Combined sources
Beta strandi254 – 2563Combined sources
Turni267 – 2726Combined sources
Beta strandi278 – 2803Combined sources
Turni291 – 2944Combined sources
Beta strandi302 – 3065Combined sources
Beta strandi324 – 3318Combined sources
Helixi339 – 3435Combined sources
Beta strandi349 – 3513Combined sources
Beta strandi371 – 3733Combined sources
Beta strandi384 – 3874Combined sources
Beta strandi395 – 3973Combined sources
Helixi407 – 4093Combined sources
Beta strandi419 – 4213Combined sources
Beta strandi424 – 4263Combined sources
Beta strandi439 – 4424Combined sources
Helixi450 – 4523Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LI1X-ray2.66A/B23-454[»]
4LI2X-ray3.19A23-454[»]
ProteinModelPortaliB0BLW3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 5934LRRNTAdd
BLAST
Repeati57 – 8125LRR 1Add
BLAST
Repeati83 – 10523LRR 2Add
BLAST
Repeati106 – 12924LRR 3Add
BLAST
Repeati131 – 15323LRR 4Add
BLAST
Repeati155 – 17723LRR 5Add
BLAST
Repeati178 – 20124LRR 6Add
BLAST
Repeati203 – 22523LRR 7Add
BLAST
Repeati226 – 24924LRR 8Add
BLAST
Repeati250 – 27223LRR 9Add
BLAST
Repeati274 – 29623LRR 10Add
BLAST
Repeati320 – 34324LRR 11Add
BLAST
Repeati345 – 36521LRR 12Add
BLAST
Repeati366 – 38924LRR 13Add
BLAST
Repeati390 – 41324LRR 14Add
BLAST
Repeati415 – 43723LRR 15Add
BLAST

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Contains 15 LRR (leucine-rich) repeats.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
KOG2087. Eukaryota.
COG4886. LUCA.
HOVERGENiHBG031675.
KOiK04309.
OrthoDBiEOG7DNNTF.
TreeFamiTF316814.

Family and domain databases

Gene3Di3.80.10.10. 3 hits.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002131. Gphrmn_rcpt_fam.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
PF13855. LRR_8. 4 hits.
[Graphical view]
PRINTSiPR00373. GLYCHORMONER.
PR00237. GPCRRHODOPSN.
SMARTiSM00369. LRR_TYP. 14 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS51450. LRR. 15 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0BLW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCPGWPLAL FALLLASCSG GPSGVSSPAP CPAPCACDLD GGADCSGKGL
60 70 80 90 100
VTVPDGLSVF THSLDLSMNN ITKLPEGAFK GFPYLEELRL AGNDLSIIHP
110 120 130 140 150
MALSGLKELK VLTLQNNQLK TVPSESLKGL VSLQSLRLDA NHIVTVPEDS
160 170 180 190 200
FEGLVQLRHL WLDDNSLTEV PIRPLSNLPS LQALTLALNK ISHIPDYAFS
210 220 230 240 250
NLSSLVVLHL HNNKIRTLGP HCFHGLDNLE ALDLNYNNLI DFPDSIRSLP
260 270 280 290 300
NLKELGFHSN SITIIPDGAF VKNPLLRTIH LYDNPLSFVG NSAFQNLSDL
310 320 330 340 350
HFLIIRGASN VQWFPNLTGT NNLESLTLTG TKIRSIPIKF CQEQKMLRTL
360 370 380 390 400
DLSYNEISAL VGFEGCSSLE EVYLQNNQIQ EVQNETFQGL AALRMLDLSR
410 420 430 440 450
NRIHTIHKEA FVTLKALTNL DLSFNDLTAF PTAGLHGLNQ LKLTGNPNFK
460 470 480 490 500
ETLTAKDLIK LSSVSVPYAY QCCAFSACNS YMTTTVEEDR LRAQRLLLDH
510 520 530 540 550
DRAAMDPDYM GTEDDKEHVQ ALIQCNPATG PFKPCEYLLG SWMIRLTVWF
560 570 580 590 600
IFLLALIFNV IVIVTMFASC SQLTSSKLFI GLIAVSNLFM GVYTGTLTVL
610 620 630 640 650
DTISWGQFAE FGIWWETGNG CKVAGFLAIF SSESAIFFLM LAAIERSLSA
660 670 680 690 700
KDIIKKEKHQ HLRKFQVASL LAVLLAAAAG CLPLFHIGEF SSSPLCLPFP
710 720 730 740 750
TGETPSLGFT VTLVLLNSLA FLIMVITYTK LYCTIEKEDL SENAESSMIK
760 770 780 790 800
HVAWLIFTNC IFFCPVAFFS FAPLITAIYI SPEIMKSVTL IFLPLPACLN
810 820 830 840 850
PVLYVFFNPK FKEDWKLLRW RLTKRSGSVA VATNSQRGCV TQDFYYDFGM
860 870 880 890 900
YSHLQGGNFA VCDYCESVLL KNPPPCKHLI KSHSCPTLAV VPCQRPDNYW
910 920 930 940 950
SEFGTQSAHS DCADEEDSFV SDSSDQVQVC GRACFYQSRG LPLVRYAYNI

PRMKD
Length:955
Mass (Da):105,725
Last modified:February 26, 2008 - v1
Checksum:iE36B9BD93557CCA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAMC01072689 Genomic DNA. No translation available.
AAMC01072690 Genomic DNA. No translation available.
AAMC01072691 Genomic DNA. No translation available.
AAMC01072692 Genomic DNA. No translation available.
AAMC01072693 Genomic DNA. No translation available.
AAMC01072694 Genomic DNA. No translation available.
AAMC01072695 Genomic DNA. No translation available.
AAMC01072696 Genomic DNA. No translation available.
BC158183 mRNA. Translation: AAI58184.1.
RefSeqiNP_001119970.1. NM_001126498.1.
UniGeneiStr.14297.

Genome annotation databases

GeneIDi100144922.
KEGGixtr:100144922.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAMC01072689 Genomic DNA. No translation available.
AAMC01072690 Genomic DNA. No translation available.
AAMC01072691 Genomic DNA. No translation available.
AAMC01072692 Genomic DNA. No translation available.
AAMC01072693 Genomic DNA. No translation available.
AAMC01072694 Genomic DNA. No translation available.
AAMC01072695 Genomic DNA. No translation available.
AAMC01072696 Genomic DNA. No translation available.
BC158183 mRNA. Translation: AAI58184.1.
RefSeqiNP_001119970.1. NM_001126498.1.
UniGeneiStr.14297.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LI1X-ray2.66A/B23-454[»]
4LI2X-ray3.19A23-454[»]
ProteinModelPortaliB0BLW3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60560N.
IntActiB0BLW3. 1 interaction.
MINTiMINT-8297617.
STRINGi8364.ENSXETP00000061102.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiB0BLW3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100144922.
KEGGixtr:100144922.

Organism-specific databases

CTDi55366.
XenbaseiXB-GENE-5959108. lgr4.

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
KOG2087. Eukaryota.
COG4886. LUCA.
HOVERGENiHBG031675.
KOiK04309.
OrthoDBiEOG7DNNTF.
TreeFamiTF316814.

Family and domain databases

Gene3Di3.80.10.10. 3 hits.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002131. Gphrmn_rcpt_fam.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
PF13855. LRR_8. 4 hits.
[Graphical view]
PRINTSiPR00373. GLYCHORMONER.
PR00237. GPCRRHODOPSN.
SMARTiSM00369. LRR_TYP. 14 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS51450. LRR. 15 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "LGR4 and LGR5 are R-spondin receptors mediating Wnt/beta-catenin and Wnt/PCP signalling."
    Glinka A., Dolde C., Kirsch N., Huang Y.L., Kazanskaya O., Ingelfinger D., Boutros M., Cruciat C.M., Niehrs C.
    EMBO Rep. 12:1055-1061(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiLGR4_XENTR
AccessioniPrimary (citable) accession number: B0BLW3
Secondary accession number(s): F6US71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: February 26, 2008
Last modified: May 11, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.