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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlamydia trachomatis serovar L2b (strain UCH-1/proctitis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei592 – 5921ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCTRA471473:GHJK-288-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CTLon_0269
OrganismiChlamydia trachomatis serovar L2b (strain UCH-1/proctitis)
Taxonomic identifieri471473 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000794 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10361036Isoleucine--tRNA ligasePRO_1000216255Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi471473.CTLon_0269.

Structurei

3D structure databases

ProteinModelPortaliB0BB05.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 5611"HIGH" regionAdd
BLAST
Motifi589 – 5935"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0BB05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNEDKISIS AKEEKILSFW KEQDIFQKTL DNREGCPTFS FYDGPPFATG
60 70 80 90 100
LPHYGHLLAG TIKDVVCRYA SMDGHYVPRR FGWDCHGVPV EYEVEKSLGL
110 120 130 140 150
TEPGAIERFG VANFNEECRK IVFRYADEWK YFVDRIGRWV DFSATWRTMD
160 170 180 190 200
LSFMESVWWV FRSLYDQGLV YEGTKVVPFS TKLGTPLSNF EAGQNYKEVD
210 220 230 240 250
DPSVVVKFAL QDNQGFLLAW TTTPWTLVSN MALAVHPELT YVRIKDKESG
260 270 280 290 300
DEYILGQESL PRWFPDRESY EWIGQLSGKS LVGQSYEPLF PYFQDKKELG
310 320 330 340 350
AFRILPADFI EESEGTGIVH MAPAFGEADF FACQEHNVPL VCPVDNQGCY
360 370 380 390 400
TAEVKDFVGE YIKSADKGIA RRLKNENKLF YQGTVRHRYP FCWRTDSPLI
410 420 430 440 450
YKAVNSWFVA VEKVKSKMLK ANESIHWTPG HIKQGRFGKW LEGARDWAIS
460 470 480 490 500
RNRYWGTPIP IWRSDDGELL VIGSIQELEA LSGQKIVDLH RHFIDEIEIN
510 520 530 540 550
QNGKSFRRIP YVFDCWFDSG AMPYAQNHYP FERAEETEAC FPADFIAEGL
560 570 580 590 600
DQTRGWFYTL TVIAAALFDQ PAFKNVIVNG IILAEDGNKM SKRLNNYPSP
610 620 630 640 650
KMIMDAYGAD ALRLYLLNSV VVKAEDLRFS DKGVESVLKQ VLLPLSNALA
660 670 680 690 700
FYKTYAELYG FDPKETDNIE LAEIDRWILS SLYSLLGKTR ESMSQYDLHA
710 720 730 740 750
AVNPFVDFIE DLTNWYIRRS RRRFWDAEDS ADRRAAFSTL YEVLVVFSKV
760 770 780 790 800
IAPFIPFISE DMYQQLRGET DPESVHLCDF PHVVLEKILP NLERKMQDIR
810 820 830 840 850
EIVALGHSLR KEHKLKVRQP LQNVYIVGSQ ERMEALAQVG SLIGEELNVK
860 870 880 890 900
DVHFCSETPE YVTTLIKPNF RTLGKKVGNR LPEIQRALAG LPQEQIQAFM
910 920 930 940 950
HKGQMVVSLG EETISLDKED ITVSWASAEG FVARSSASFV AVLDCQLTEP
960 970 980 990 1000
LIMEGIAREL VNKINTMRRN GKLHVSDRIA IRLHAPVIVQ EAFALHKEYI
1010 1020 1030
CEETLTTSVS VIDYKEGEEW DINGHAVSFV LERVER
Length:1,036
Mass (Da):118,774
Last modified:February 25, 2008 - v1
Checksum:i3EC21D4DEC10D32B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM884177 Genomic DNA. Translation: CAP06667.1.
RefSeqiYP_001653370.1. NC_010280.2.

Genome annotation databases

EnsemblBacteriaiCAP06667; CAP06667; CTLon_0269.
KEGGictl:CTLon_0269.
PATRICi20382111. VBIChlTra68089_0297.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM884177 Genomic DNA. Translation: CAP06667.1.
RefSeqiYP_001653370.1. NC_010280.2.

3D structure databases

ProteinModelPortaliB0BB05.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi471473.CTLon_0269.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAP06667; CAP06667; CTLon_0269.
KEGGictl:CTLon_0269.
PATRICi20382111. VBIChlTra68089_0297.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciCTRA471473:GHJK-288-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UCH-1/proctitis.

Entry informationi

Entry nameiSYI_CHLTB
AccessioniPrimary (citable) accession number: B0BB05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2009
Last sequence update: February 25, 2008
Last modified: March 31, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.