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B0B9C6

- SYI_CHLT2

UniProt

B0B9C6 - SYI_CHLT2

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei592 – 5921ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCTRA471472:GJ9M-289-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CTL0274
OrganismiChlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B)
Taxonomic identifieri471472 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000795: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10361036Isoleucine--tRNA ligasePRO_1000216253Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi471472.CTL0274.

Structurei

3D structure databases

ProteinModelPortaliB0B9C6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 5611"HIGH" regionAdd
BLAST
Motifi589 – 5935"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0B9C6 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDNEDKISIS AKEEKILSFW KEQDIFQKTL DNREGCPTFS FYDGPPFATG
60 70 80 90 100
LPHYGHLLAG TIKDVVCRYA SMDGHYVPRR FGWDCHGVPV EYEVEKSLGL
110 120 130 140 150
TEPGAIERFG VANFNEECRK IVFRYADEWK YFVDRIGRWV DFSATWRTMD
160 170 180 190 200
LSFMESVWWV FRSLYDQGLV YEGTKVVPFS TKLGTPLSNF EAGQNYKEVD
210 220 230 240 250
DPSVVVKFAL QDNQGFLLAW TTTPWTLVSN MALAVHPELT YVRIKDKESG
260 270 280 290 300
DEYILGQESL PRWFPDRESY EWIGQLSGKS LVGQSYEPLF PYFQDKKELG
310 320 330 340 350
AFRILPADFI EESEGTGIVH MAPAFGEADF FACQEHNVPL VCPVDNQGCY
360 370 380 390 400
TAEVKDFVGE YIKSADKGIA RRLKNENKLF YQGTVRHRYP FCWRTDSPLI
410 420 430 440 450
YKAVNSWFVA VEKVKSKMLK ANESIHWTPG HIKQGRFGKW LEGARDWAIS
460 470 480 490 500
RNRYWGTPIP IWRSDDGELL VIGSIQELEA LSGQKIVDLH RHFIDEIEIN
510 520 530 540 550
QNGKSFRRIP YVFDCWFDSG AMPYAQNHYP FERAEETEAC FPADFIAEGL
560 570 580 590 600
DQTRGWFYTL TVIAAALFDQ PAFKNVIVNG IILAEDGNKM SKRLNNYPSP
610 620 630 640 650
KMIMDAYGAD ALRLYLLNSV VVKAEDLRFS DKGVESVLKQ VLLPLSNALA
660 670 680 690 700
FYKTYAELYG FDPKETDNIE LAEIDRWILS SLYSLLGKTR ESMSQYDLHA
710 720 730 740 750
AVNPFVDFIE DLTNWYIRRS RRRFWDAEDS TDRRAAFSTL YEVLVVFSKV
760 770 780 790 800
IAPFIPFISE DMYQQLRGET DPESVHLCDF PHVVLEKILP NLERKMQDIR
810 820 830 840 850
EIVALGHSLR KEHKLKVRQP LQNVYIVGSQ ERMEALAQVG SLIGEELNVK
860 870 880 890 900
DVHFCSETPE YVTTLIKPNF RTLGKKVGNR LPEIQRALAG LPQEQIQAFM
910 920 930 940 950
HKGQMVVSLG EETISLDKED ITVSWASAEG FVARSSASFV AVLDCQLTEP
960 970 980 990 1000
LIMEGIAREL VNKINTMRRN GKLHVSDRIA IRLHAPVIVQ EAFALHKEYI
1010 1020 1030
CEETLTTSVS VIDYKEGEEW DINGHAVSFV LERVER
Length:1,036
Mass (Da):118,804
Last modified:February 26, 2008 - v1
Checksum:i37BDB13DEAC3062B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM884176 Genomic DNA. Translation: CAP03713.1.
RefSeqiYP_001654358.1. NC_010287.1.

Genome annotation databases

EnsemblBacteriaiCAP03713; CAP03713; CTL0274.
GeneIDi5858570.
KEGGictb:CTL0274.
PATRICi20376044. VBIChlTra32542_0298.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM884176 Genomic DNA. Translation: CAP03713.1 .
RefSeqi YP_001654358.1. NC_010287.1.

3D structure databases

ProteinModelPortali B0B9C6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 471472.CTL0274.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAP03713 ; CAP03713 ; CTL0274 .
GeneIDi 5858570.
KEGGi ctb:CTL0274.
PATRICi 20376044. VBIChlTra32542_0298.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CTRA471472:GJ9M-289-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 434/Bu / ATCC VR-902B.

Entry informationi

Entry nameiSYI_CHLT2
AccessioniPrimary (citable) accession number: B0B9C6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 26, 2008
Last modified: October 29, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3