B0B8L8 (B0B8L8_CHLT2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL CAP03455.1

Gene names

Name:	topA EMBL CAP03455.1
Ordered Locus Names:	CTL0011

Organism

Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B) [Complete proteome] [HAMAP] EMBL CAP03455.1

Taxonomic identifier

471472 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	857 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B0B8L8 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 923CE42248BFF19F	FASTA	857	96,705
	10 20 30 40 50 60 MKKSLIIVES PAKIKTLRKL LGEGFIFDSS LGHIVDLPAK GFGIDIENGF VPDYQILEGK 70 80 90 100 110 120 EEVIRKICAE AKKCDVVYLA PDPDREGEAI AWHIANQLPK DTKIQRISFN AITKGAVTEA 130 140 150 160 170 180 LKHPREIDMA LVNAQQARRF LDRIVGYKIS PILGRKLQRW SGVSAGRVQS VALKLVVDRE 190 200 210 220 230 240 YAIERFVPVE FWNIRVHLKD PQTQKTFWAH LHSVNGKKWE KEIPEGKTSD EVILIDSKEK 250 260 270 280 290 300 ADEIVALLES ATYVVDRVES KEKKRHAYPP FITSTLQQEA SRHYRFSSSR TMNIAQTLYE 310 320 330 340 350 360 GVDLDSQGAV GLITYMRTDS VRTDPEAVKQ VRKYIEGHFG KEFVPSSPNV YATKKMAQDA 370 380 390 400 410 420 HEAIRPTDVT ITPESIRSKL TEDQYKLYSL IWKRFVASQM ISAIYDTLAI RITTNKGIDL 430 440 450 460 470 480 RATGSCLKFK GFLAVYEEKR DEEGDEEENI HLPKLNERDV LTKEELEAEQ SHTKPLPRFT 490 500 510 520 530 540 EASLVKELEK SGIGRPSTYA TIMNKIQSRE YTLKEGQRLR PTELGKVVCQ FLETNFPRIM 550 560 570 580 590 600 DIGFTAGMED ELELIADNKK PWKQLLQEFC ELFLPFVVTA EKEAFIPRIV TEIDCPKCHK 610 620 630 640 650 660 GKLVKIWAKN RYFFGCSEYP TCDYKTSEEE LTFDKNEYAE DTPWDAPCAL CGGEMKVRHG 670 680 690 700 710 720 KFGSFLGCEN YPKCHYIVNL FKKGEAGAEP EATVHCPAEG CTGHLVKRRS RFNKMFYSCS 730 740 750 760 770 780 EYPACSVIGN SVDAVIEKYA GTPKTPYEKK PKAKKSIAST KGKAAKTVKK SSATTKKRAT 790 800 810 820 830 840 KAYTPSAALA AVIGADPVGR PEATKKLWEY IKEKGLQSPQ NKKIIIPDSK LQGVIGADPI 850 DMFALSKKLS AHLIKEE

References

[1]

"Chlamydia trachomatis: genome sequence analysis of lymphogranuloma venereum isolates." Thomson N.R., Holden M.T.G., Carder C., Lennard N., Lockey S.J., Marsh P., Skipp P., O'Connor C.D., Goodhead I., Norbertzcak H., Harris B., Ormond D., Rance R., Quail M.A., Parkhill J., Stephens R.S., Clarke I.N. Genome Res. 18:161-171(2008) [PubMed: 18032721] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM884176 Genomic DNA. Translation: CAP03455.1.
RefSeq	YP_001654102.1. NC_010287.1.
3D structure databases
ProteinModelPortal	B0B8L8.
ModBase	Search...
Protein-protein interaction databases
STRING	B0B8L8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5858735.
GenomeReviews	Gene locus CTL0011 in contig AM884176_GR.
KEGG	ctb:CTL0011.
PATRIC	20375436. VBIChlTra32542_0012.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	PRK06319.
Family and domain databases
InterPro	IPR019835. SWIB_domain. IPR003121. SWIB_MDM2_domain. IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF02201. SWIB. 1 hit. PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00151. SWIB. 1 hit. SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF47592. MDM2. 1 hit. SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B0B8L8_CHLT2

Accession

Primary (citable) accession number: B0B8L8

Entry history

Integrated into UniProtKB/TrEMBL:	February 26, 2008
Last sequence update:	February 26, 2008
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)