B0B7L3 (RNC_CHLT2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	CTL0549

Organism

Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B) [Complete proteome] [HAMAP]

Taxonomic identifier

471472 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chlamydiae/Verrucomicrobia group › Chlamydiae › Chlamydiia › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia › Chlamydia trachomatis

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg2+ By similarity. HAMAP-Rule MF_00104
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00104
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00104.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding RNA-binding rRNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Ribonuclease 3 HAMAP-Rule MF_00104		PRO_1000094099
Regions
	Domain	7 – 135	129	RNase III
	Domain	160 – 229	70	DRBM
Sites
	Active site	52	1	Potential
	Active site	124	1	By similarity
	Metal binding	48	1	Magnesium By similarity
	Metal binding	121	1	Magnesium By similarity
	Metal binding	124	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0B7L3 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 9AC603E1DB831C6A	FASTA	231	25,595
	10 20 30 40 50 60 MQHTVDIQAI ESKLNFTFSH PRLLITALTH PSYRNEFPSA EEDSERLEFL GDAVLGLVVT 70 80 90 100 110 120 EHLFLLFPAL NEGLLSTTRA ALVNAEACFE YTQKLSLGEH LLIGRGEKMQ SHRGKISAYA 130 140 150 160 170 180 NLFEAILGAV YLDGGLSPAR QIIVPLLPDK ESILPLMLVN PKNRLQQFTQ QTLKVLPSYK 190 200 210 220 230 ALPWKSEDGS PGYHVQVFVN GDLWGEGFAG SKKEAEKLAA KQALSTHDNK N

References

[1]

"Chlamydia trachomatis: genome sequence analysis of lymphogranuloma venereum isolates." Thomson N.R., Holden M.T.G., Carder C., Lennard N., Lockey S.J., Marsh P., Skipp P., O'Connor C.D., Goodhead I., Norbertzcak H., Harris B., Ormond D., Rance R., Quail M.A., Parkhill J., Stephens R.S., Clarke I.N. Genome Res. 18:161-171(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 434/Bu / ATCC VR-902B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM884176 Genomic DNA. Translation: CAP03989.1.
RefSeq	YP_001654626.1. NC_010287.1.
3D structure databases
ProteinModelPortal	B0B7L3.
ModBase	Search...
Protein-protein interaction databases
STRING	471472.CTL0549.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAP03989; CAP03989; CTL0549.
GeneID	5858279.
KEGG	ctb:CTL0549.
PATRIC	20376648. VBIChlTra32542_0590.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0571.
HOGENOM	HOG000246808.
KO	K03685.
OMA	SAYANLF.
ProtClustDB	PRK00102.
Enzyme and pathway databases
BioCyc	CTRA471472:GJ9M-575-MONOMER.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit. 3.30.160.20. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_CHLT2

Accession

Primary (citable) accession number: B0B7L3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 26, 2008
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program