B0AYL1 (B0AYL1_BACAN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL EDR16519.1
ORF Names:	BAC_3980 EMBL EDR16519.1

Organism

Bacillus anthracis str. A0488 EMBL EDR16519.1

Taxonomic identifier

486624 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	692 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733 RuleBase RU000518
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B0AYL1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 9EBA587C6F59848E	FASTA	692	79,064
	10 20 30 40 50 60 MSDYLVIVES PSKAKTIEKY LGKKYKVVAS MGHVRDLPKS QMGIEVKNNF TPKYITIRGK 70 80 90 100 110 120 GPVLKDLKSA AKKAKKVYLA ADPDREGEAI AWHLANTLNV DVESDCRVVF NEITKDAIKE 130 140 150 160 170 180 SFKHPRAINM DLVDAQQARR ILDRLVGYNI SPLLWKKVKK GLSAGRVQSV AVRLIIERER 190 200 210 220 230 240 EIQSFEPEEF WTIKTEFVKG KDTFEASFYG VDGEKVQLTN ETQVNEIIEQ LKDNAFSVEN 250 260 270 280 290 300 VTRKERKRNP ALPFTTSSLQ QEAARKLNMR AKKTMMLAQQ LYEGIDLGKQ GTVGLITYMR 310 320 330 340 350 360 TDSTRISETA QTEARTYITE AYGTEYIGAE KKKETKKSNA QDAHEAIRPT SVMRKPEELK 370 380 390 400 410 420 SFLSRDQLRL YKLIWERFVA SQMASAIMDT VTARLINNNV QFRASGSVVK FPGFMKVYVE 430 440 450 460 470 480 SKDDGAEEKD KMLPPLEVGE TVFSKDLEPK QHFTQPPPRY TEARLVRTLE ELGIGRPSTY 490 500 510 520 530 540 VPTLETIQKR GYVGLDNKRF VPTELGEIVI ELILEFFPEI INIEFTANME QSLDEVEEGN 550 560 570 580 590 600 ANWVKIVDDF YVGFEPRLEK AEKEMREVEI KDEPAGEDCE LCNHPMVFKM GKYGKFMACS 610 620 630 640 650 660 NFPDCRNTKP IVKEIGVTCP KCDKGQIIER RSNKKKRLFY GCGTYPECDF VSWDKPIGRK 670 680 690 CPKCEGMLVE KKLKKGVQVQ CISCDYEEEQ QM

References

[1]	"Genome sequence of Bacillus anthracis A0488." Dodson R.J., Munk A.C., Tapia R., Green L., Rogers Y., Detter J.C., Bruce D., Sutton G., Brettin T.S. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: A0488 EMBL EDR16519.1.
[2]	Munk A.C., Tapia R., Green L., Rogers Y., Detter J.C., Bruce D., Brettin T.S. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: A0488 EMBL EDR16519.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ABJC01000056 Genomic DNA. Translation: EDR16519.1.
3D structure databases
ProteinModelPortal	B0AYL1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
PATRIC	24649358. VBIBacAnt7575_5529.
Phylogenomic databases
OMA	EEYWSID.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B0AYL1_BACAN

Accession

Primary (citable) accession number: B0AYL1

Entry history

Integrated into UniProtKB/TrEMBL:	February 26, 2008
Last sequence update:	February 26, 2008
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)