Reviewed, UniProtKB/Swiss-Prot Q0JF21 (AGI_ORYSJ)

Names and origin

Protein names

Recommended name:
    Lectin
Alternative name(s):
    Agglutinin
Cleaved into the following 2 chains:
    1- Recommended name:
            Lectin 10 kDa peptide
    2- Recommended name:
            Lectin 8 kDa peptide

Gene names

Ordered Locus Names:	Os04g0173800, LOC_Os04g09390
ORF Names:	OSJNBb0015C06.8

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	N-acetyl-D-glucosamine binding lectin.
Tissue specificity	Confined to root caps, several cell layers at the periphery of the coleorhiza and radicle, and in all cell layers of the coleoptile.
Sequence similarities	Contains 4 chitin-binding type-1 domains.

Ontologies

Keywords
Domain	Repeat Signal
Ligand	Chitin-binding Lectin
PTM	Glycoprotein Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	chitin binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28
	Chain	29 – 201	173	Lectin		PRO_0000005262
	Chain	29 – 122	94	Lectin 10 kDa peptide		PRO_0000005263
	Chain	123 – 201	79	Lectin 8 kDa peptide		PRO_0000005264
	Propeptide	202 – 227	26			PRO_0000005265
Regions
	Domain	29 – 70	42	Chitin-binding type-1 1
	Domain	71 – 113	43	Chitin-binding type-1 2
	Domain	114 – 156	43	Chitin-binding type-1 3
	Domain	157 – 199	43	Chitin-binding type-1 4
	Region	38 – 40	3	Substrate binding By similarity
	Region	90 – 101	12	Substrate binding By similarity
	Region	142 – 143	2	Substrate binding By similarity
Amino acid modifications
	Modified residue	29	1	Pyrrolidone carboxylic acid
	Glycosylation	211	1	N-linked (GlcNAc...) Potential
	Disulfide bond	31 ↔ 46		By similarity
	Disulfide bond	40 ↔ 52		By similarity
	Disulfide bond	45 ↔ 59		By similarity
	Disulfide bond	63 ↔ 68		By similarity
	Disulfide bond	74 ↔ 89		By similarity
	Disulfide bond	83 ↔ 95		By similarity
	Disulfide bond	88 ↔ 102		By similarity
	Disulfide bond	106 ↔ 111		By similarity
	Disulfide bond	117 ↔ 132		By similarity
	Disulfide bond	126 ↔ 138		By similarity
	Disulfide bond	131 ↔ 145		By similarity
	Disulfide bond	149 ↔ 154		By similarity
	Disulfide bond	160 ↔ 175		By similarity
	Disulfide bond	169 ↔ 181		By similarity
	Disulfide bond	174 ↔ 188		By similarity
	Disulfide bond	192 ↔ 197		By similarity
Experimental info
	Sequence conflict	119	1	R → S in AAD27887. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q0JF21-1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 691EB39F6690BAF1	FASTA	227	22,796
	10 20 30 40 50 60 MTMTSTTTKA MAMAAAVLAA AAVAATNAQT CGKQNDGMIC PHNLCCSQFG YCGLGRDYCG 70 80 90 100 110 120 TGCQSGACCS SQRCGSQGGG ATCSNNQCCS QYGYCGFGSE YCGSGCQNGP CRADIKCGRN 130 140 150 160 170 180 ANGELCPNNM CCSQWGYCGL GSEFCGNGCQ SGACCPEKRC GKQAGGDKCP NNFCCSAGGY 190 200 210 220 CGLGGNYCGS GCQSGGCYKG GDGMAAILAN NQSVSFEGII ESVAELV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of rice lectin is governed by two temporally and spatially regulated mRNAs in developing embryos." Wilkins T.A., Raikhel N.V. Plant Cell 1:541-549(1989) [PubMed: 2535550] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, TISSUE SPECIFICITY. Strain: cv. Lemont.
[2]	"Sequence and analysis of rice chromosome 4." Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B. Nature 420:316-320(2002) [PubMed: 12447439] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"Molecular evolution of lectin gene in the genus Oryza." Hao Z., Liu Q., Shen D. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-227.
[6]	"Extensive homologies between lectins from non-leguminous plants." Chapot M.-P., Peumans W.J., Strosberg A.D. FEBS Lett. 195:231-234(1986) Cited for: PRELIMINARY PROTEIN SEQUENCE OF 123-157.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M24504 mRNA. Translation: AAA20873.1. AL662990 Genomic DNA. Translation: CAE02230.2. Different initiation. AP008210 Genomic DNA. Translation: BAF14066.1. AF140679 Genomic DNA. Translation: AAD27887.1. AF140682 Genomic DNA. Translation: AAD27890.1.
PIR	A23616. LNRZ. JQ1102.
RefSeq	NP_001052152.1.
UniGene	Os.54304
3D structure databases
HSSP	HSSP built from PDB template 2CWG based on UniProtKB P10968.
SMR	Q0JF21. Positions 29-197.
ModBase	Search...
Genome annotation databases
GeneID	4335068.
KEGG	osa:4335068.
Organism-specific databases
Gramene	P11219.
Family and domain databases
InterPro	IPR001002. Chitin_bd_1. IPR000726. Glyco_hydro_19_cat. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 4 hits.
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00187. Chitin_bind_1. 4 hits. [Graphical view]
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_binding_1. 4 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 4 hits. [Graphical view]
PROSITE	PS00026. CHIT_BIND_I_1. 4 hits. PS50941. CHIT_BIND_I_2. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGI_ORYSJ

Accession

Primary (citable) accession number: Q0JF21
Secondary accession number(s): P11219 , Q7FZU3, Q7XRY8, Q9SBW5, Q9XFF3, Q9XFF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	October 3, 2006
Last modified:	November 25, 2008
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)