Adagio protein 1 - Arabidopsis thaliana (Mouse-ear cress)

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Reviewed, UniProtKB/Swiss-Prot Q94BT6 (ADO1_ARATH)

Last modified November 25, 2008. Version 49. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Adagio protein 1
Alternative name(s):
    Protein ZEITLUPE
    Clock-associated PAS protein ZTL
    LOV kelch protein 1
    Flavin-binding kelch repeat F-box protein 1-like protein 2
      Short name=FKF1-like protein 2
    F-box only protein 2b
      Short name=FBX2b

Gene names

Name:	ADO1
Synonyms:	FKL2, LKP1, ZTL
Ordered Locus Names:	At5g57360
ORF Names:	MSF19.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Component of E3 ubiquitin ligase complexes that plays a central role blue-light-dependent circadian cycles. Acts as a blue-light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO1) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including transition to flowering time, hypocotyl elongation, cotyledons and leaves movement rhythms. APRR1 and APRR5 seem to be substrates of the SCF(ADO1) complex.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with ADO2. Component of an E3 ubiquitin ligase SCF(ADO1) complex composed of SKP1A/ASK1 (or SKP1B/ASK2), CUL1, RBX1 and ADO1. Also interacts with SKP1D/ASK4, SKP1K/ASK11, CRY1, PHYB, APRR1 and APRR5, and probably with SKP1N/ASK14 and SKP1S/ASK19.
Subcellular location	Nucleus. Cytoplasm. Note= Nuclear after 9 hours of illumination (afternoon of long days). Cytoplasmic when plant have been subsequently grown 16 hours in light and 5 hours in dark (early morning of long days).
Tissue specificity	Ubiquitously expressed with higher levels in cotyledons and leaves.
Developmental stage	Mainly present during the light phase, and degraded in a proteasome-dependent manner in dark (at protein level).
Post-translational modification	May be ubiquitinated. Degraded in a proteasome-dependent manner. FMN binds covalently to cysteine after exposure to blue light and is reversed in the dark By similarity.
Miscellaneous	'Zeitlupe' means slow motion in German. 'Adagio' means slowly in Italian.
Sequence similarities	Belongs to the ADAGIO family. Contains 1 F-box domain. Contains 5 Kelch repeats. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain.
Sequence caution	The sequence AAK64006.1 differs from that shown. Reason: Frameshift at positions 462 and 467.

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Ontologies

Keywords
Biological process	Biological rhythms Sensory transduction Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Domain	Kelch repeat Repeat
Ligand	Chromophore FMN Flavoprotein
Molecular function	Photoreceptor protein Receptor
PTM	Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW rhythmic process Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Inferred from electronic annotation. Source: InterPro ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	photoreceptor activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.4 Ref.8 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
APRR1	Q9LKL2	4	EBI-300691,EBI-618423
AT4g34210	O49484	2	EBI-300691,EBI-401185
CRY1	Q43125	1	EBI-300691,EBI-300703
GI	Q9SQI2	4	EBI-300691,EBI-446380
PHYB	P14713	1	EBI-300691,EBI-300727
SKP1A	Q39255	4	EBI-300691,EBI-532357
SKP1B	Q9FHW7	2	EBI-300691,EBI-604076

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 609

609

Adagio protein 1

PRO_0000119956

Regions

Domain

32 – 114

PAS

Domain

118 – 161

PAC

Domain

195 – 241

F-box

Repeat

292 – 342

Kelch 1

Repeat

345 – 392

Kelch 2

Repeat

397 – 445

Kelch 3

Repeat

450 – 501

Kelch 4

Repeat

516 – 564

Kelch 5

Amino acid modifications

Modified residue

S-4a-FMN cysteine By similarity

Experimental info

Mutagenesis

200

L → A: No SCF(ADO1) complex formation and reduced cyclic degradation of ADO1; when associated with A-213

Mutagenesis

213

L → A: No SCF(ADO1) complex formation and reduced cyclic degradation of ADO1; when associated with A-200

Mutagenesis

320

D → N in ztl-2; affects circadian clock by lengthening the free-running period of clock-controlled processes

Mutagenesis

425

D → N in ztl-1; affects circadian clock by lengthening the free-running period of clock-controlled processes

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Sequences

Sequence

Length

Mass (Da)

Tools

Q94BT6-1 [UniParc].

Last modified November 8, 2005. Version 2.
Checksum: B25192FCBE019093
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FASTA

609

65,906

        10         20         30         40         50         60 
MEWDSGSDLS ADDASSLADD EEGGLFPGGG PIPYPVGNLL HTAPCGFVVT DAVEPDQPII 

        70         80         90        100        110        120 
YVNTVFEMVT GYRAEEVLGG NCRFLQCRGP FAKRRHPLVD SMVVSEIRKC IDEGIEFQGE 

       130        140        150        160        170        180 
LLNFRKDGSP LMNRLRLTPI YGDDDTITHI IGIQFFIETD IDLGPVLGSS TKEKSIDGIY 

       190        200        210        220        230        240 
SALAAGERNV SRGMCGLFQL SDEVVSMKIL SRLTPRDVAS VSSVCRRLYV LTKNEDLWRR 

       250        260        270        280        290        300 
VCQNAWGSET TRVLETVPGA KRLGWGRLAR ELTTLEAAAW RKLSVGGSVE PSRCNFSACA 

       310        320        330        340        350        360 
VGNRVVLFGG EGVNMQPMND TFVLDLNSDY PEWQHVKVSS PPPGRWGHTL TCVNGSNLVV 

       370        380        390        400        410        420 
FGGCGQQGLL NDVFVLNLDA KPPTWREISG LAPPLPRSWH SSCTLDGTKL IVSGGCADSG 

       430        440        450        460        470        480 
VLLSDTFLLD LSIEKPVWRE IPAAWTPPSR LGHTLSVYGG RKILMFGGLA KSGPLKFRSS 

       490        500        510        520        530        540 
DVFTMDLSEE EPCWRCVTGS GMPGAGNPGG VAPPPRLDHV AVNLPGGRIL IFGGSVAGLH 

       550        560        570        580        590        600 
SASQLYLLDP TEDKPTWRIL NIPGRPPRFA WGHGTCVVGG TRAIVLGGQT GEEWMLSELH 


ELSLASYLT

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ZEITLUPE encodes a novel clock-associated PAS protein from Arabidopsis." Somers D.E., Schultz T.F., Milnamow M., Kay S.A. Cell 101:319-329(2000) [PubMed: 10847686] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, MUTAGENESIS OF ASP-320 AND ASP-425.
[2]	"FKF1, a clock-controlled gene that regulates the transition to flowering in Arabidopsis." Nelson D.C., Lasswell J.E., Rogg L.E., Cohen M.A., Bartel B. Cell 101:331-340(2000) [PubMed: 10847687] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[3]	"LKP1 (LOV kelch protein 1): a factor involved in the regulation of flowering time in Arabidopsis." Kiyosue T., Wada M. Plant J. 23:807-815(2000) [PubMed: 10998191] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION.
[4]	"An Arabidopsis circadian clock component interacts with both CRY1 and phyB." Jarillo J.A., Capel J., Tang R.-H., Yang H.-Q., Alonso J.M., Ecker J.R., Cashmore A.R. Nature 410:487-490(2001) [PubMed: 11260718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CRY1 AND PHYB.
[5]	"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones." Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S. DNA Res. 5:379-391(1998) [PubMed: 10048488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[7]	"F-box proteins in Arabidopsis." Xiao W., Jang J.-C. Trends Plant Sci. 5:454-457(2000) [PubMed: 11077244] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[8]	"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis." Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L. Plant J. 34:753-767(2003) [PubMed: 12795696] [Abstract] Cited for: INTERACTION WITH SKP1A; SKP1B; SKP1D; SKP1K AND SKP1S.
[9]	"Circadian phase-specific degradation of the F-box protein ZTL is mediated by the proteasome." Kim W.-Y., Geng R., Somers D.E. Proc. Natl. Acad. Sci. U.S.A. 100:4933-4938(2003) [PubMed: 12665620] [Abstract] Cited for: INDUCTION.
[10]	"Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis." Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T. J. Exp. Bot. 55:2015-2027(2004) [PubMed: 15310821] [Abstract] Cited for: INTERACTION WITH SKP1A; SKP1B; SKP1D; SKP1K; SKP1N; ADO2; APRR1 AND APRR5.
[11]	"The F-box protein ZEITLUPE confers dosage-dependent control on the circadian clock, photomorphogenesis, and flowering time." Somers D.E., Kim W.-Y., Geng R. Plant Cell 16:769-782(2004) [PubMed: 14973171] [Abstract] Cited for: FUNCTION.
[12]	"Formation of an SCF(ZTL) complex is required for proper regulation of circadian timing." Han L., Mason M., Risseeuw E.P., Crosby W.L., Somers D.E. Plant J. 40:291-301(2004) [PubMed: 15447654] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF LEU-200 AND LEU-213, IDENTIFICATION IN A SCF(ADO1) COMPLEX.

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Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

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Cross-references

Sequence databases
	AF254413 mRNA. Translation: AAF70288.1. AF216525 mRNA. Translation: AAF32300.1. AB038796 mRNA. Translation: BAB18914.1. AF252294 mRNA. Translation: AAK27433.1. AB016891 Genomic DNA. Translation: BAB08473.1. AY039902 mRNA. Translation: AAK64006.1. Frameshift. BT008772 mRNA. Translation: AAP68211.1.
RefSeq	NP_568855.1.
UniGene	At.21022
3D structure databases
HSSP	HSSP built from PDB template 1N9L based on UniProtKB Q8LPE0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q94BT6.
Genome annotation databases
GeneID	835842.
GenomeReviews	Gene locus AT5G57360 in contig BA000015_GR.
KEGG	ath:AT5G57360.
NMPDR	fig\|3702.1.peg.27688.
Organism-specific databases
GeneFarm	5106. 485.
TAIR	At5g57360.
Gene expression databases
GermOnline	AT5G57360. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001810. F-box. IPR015915. Kelch-typ_b-propeller. IPR006652. Kelch_1. IPR011498. Kelch_2. IPR001610. PAC. IPR000014. PAS. IPR000700. PAS-assoc_C. [Graphical view]
Gene3D	G3DSA:2.120.10.80. Kelch-typ_b-propeller. 1 hit.
Pfam	PF00646. F-box. 1 hit. PF01344. Kelch_1. 1 hit. PF07646. Kelch_2. 4 hits. [Graphical view]
SMART	SM00256. FBOX. 1 hit. SM00086. PAC. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. 1 hit. PS50113. PAC. False negative. PS50112. PAS. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

ADO1_ARATH

Accession

Primary (citable) accession number: Q94BT6
Secondary accession number(s): Q9LDF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	November 25, 2008
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Web resources

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents