A9XE60 (KBX11_MESEU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Potassium channel toxin MeuTXK-beta-1 Short name=MeuTXKbeta1
Organism	Mesobuthus eupeus (Lesser Asian scorpion) (Buthus eupeus)
Taxonomic identifier	34648 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Mesobuthus

Protein attributes

Sequence length	91 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a low affinity binding to potassium channels of rat brain synaptosomes. Displays weak antibacterial activity against Stenotrophomonas sp. Strongly inhibits the development of the Plasmodium berghei ookinetes. Displays slight hemolytic effect on mouse erythrocytes. Induces cytolysis on Xenopus oocytes at high concentrations. Is not toxic on mice and on the insect Tenebrio molitor. Ref.1
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the long chain scorpion toxin family. Class 1 subfamily.
Mass spectrometry	Molecular mass is 8256.81 Da from positions 20 - 91. Determined by MALDI. Ref.1

Ontologies

Keywords
Biological process	Cytolysis Hemolysis
Cellular component	Secreted
Domain	Signal
Molecular function	Antibiotic Antimicrobial Ion channel impairing toxin Potassium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW hemolysis in other organism Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.1
	Chain	20 – 91	72	Potassium channel toxin MeuTXK-beta-1		PRO_0000394022
Amino acid modifications
	Disulfide bond	57 ↔ 78		By similarity
	Disulfide bond	64 ↔ 83		By similarity
	Disulfide bond	68 ↔ 85		By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9XE60 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: C2879665D440C682	FASTA	91	10,338
	10 20 30 40 50 60 MQRNLVVLLF LGMVALSSCG FREKHFQRFV KYAVPESTLR TVLQTVVHKV GKTQFGCPAY 70 80 90 QGYCDDHCQD IEKKEGFCHG FKCKCGIPMG F

References

[1]

"MeuTXKbeta1, a scorpion venom-derived two-domain potassium channel toxin-like peptide with cytolytic activity." Zhu S., Gao B., Aumelas A., Del Carmen Rodriguez M., Lanz-Mendoza H., Peigneur S., Diego-Garcia E., Martin-Eauclaire M.-F., Tytgat J., Possani L.D. Biochim. Biophys. Acta 1804:872-883(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-23, FUNCTION, MASS SPECTROMETRY. Tissue: Venom and Venom gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF190325 mRNA. Translation: ABP35519.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

KBX11_MESEU

Accession

Primary (citable) accession number: A9XE60

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 18, 2010
Last sequence update:	February 5, 2008
Last modified:	March 6, 2013
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program