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A9X4C2

- A9X4C2_9ENTO

UniProt

A9X4C2 - A9X4C2_9ENTO

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Protein

Genome polyprotein

Gene
N/A
Organism
Enterovirus A71
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

NTP + H2O = NDP + phosphate.SAAS annotation
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SAAS annotation
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.SAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi136 – 1361CalciumImported
Metal bindingi225 – 2251CalciumImported

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. ion channel activity Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW
  5. RNA binding Source: UniProtKB-KW
  6. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  7. RNA helicase activity Source: InterPro
  8. structural molecule activity Source: InterPro

GO - Biological processi

  1. pore formation by virus in membrane of host cell Source: UniProtKB-KW
  2. protein oligomerization Source: UniProtKB-KW
  3. RNA-protein covalent cross-linking Source: InterPro
  4. transcription, DNA-templated Source: InterPro
  5. viral entry into host cell Source: UniProtKB-KW
  6. viral RNA genome replication Source: InterPro
  7. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

HelicaseSAAS annotation, Hydrolase, Ion channel, Nucleotidyltransferase, Protease, RNA-directed RNA polymeraseSAAS annotation, Thiol proteaseSAAS annotation, Transferase, Viral ion channelSAAS annotation

Keywords - Biological processi

Host-virus interaction, Ion transport, Transport, Viral attachment to host cellSAAS annotation, Viral RNA replicationSAAS annotation, Virus entry into host cell

Keywords - Ligandi

ATP-binding, CalciumImported, Metal-bindingImported, Nucleotide-binding, RNA-bindingSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyproteinSAAS annotation
OrganismiEnterovirus A71Imported
Taxonomic identifieri39054 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesPicornaviridaeEnterovirusEnterovirus A

Subcellular locationi

Host cytoplasm SAAS annotation
Host cytoplasmic vesicle membrane SAAS annotation; Peripheral membrane protein SAAS annotation; Cytoplasmic side SAAS annotation

GO - Cellular componenti

  1. host cell cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  2. integral to membrane of host cell Source: UniProtKB-KW
  3. membrane Source: UniProtKB-KW
  4. viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid proteinSAAS annotation, Host cytoplasmSAAS annotation, Host cytoplasmic vesicleSAAS annotation, Host membraneSAAS annotation, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6969VP4ImportedPRO_5000300266Add
BLAST
Chaini70 – 323254VP2ImportedPRO_5000300267Add
BLAST
Chaini324 – 565242VP3ImportedPRO_5000300268Add
BLAST
Chaini566 – 862297VP1ImportedPRO_5000300269Add
BLAST
Chaini863 – 10121502AImportedPRO_5000300270Add
BLAST
Chaini1013 – 1111992BImportedPRO_5000300271Add
BLAST
Chaini1112 – 14403292CImportedPRO_5000300272Add
BLAST
Chaini1441 – 1526863AImportedPRO_5000300273Add
BLAST
Chaini1527 – 1548223BImportedPRO_5000300274Add
BLAST
Chaini1549 – 17311833CImportedPRO_5000300275Add
BLAST
Chaini1732 – 21934623DImportedPRO_5000300276Add
BLAST

Keywords - PTMi

PhosphoproteinSAAS annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZFEX-ray2.70A566-863[»]
B70-323[»]
C324-565[»]
D1-69[»]
3ZFFX-ray3.40A566-862[»]
B70-323[»]
C324-565[»]
D1-69[»]
3ZFGX-ray3.20A566-863[»]
B70-323[»]
C324-565[»]
D1-69[»]
4AEDX-ray3.80A566-862[»]
B70-323[»]
C324-565[»]
D1-69[»]
4C0Uelectron microscopy10.00A566-863[»]
B70-323[»]
C324-565[»]
4C0Yelectron microscopy16.00A566-863[»]
B70-323[»]
C324-565[»]
4C10electron microscopy13.00A566-863[»]
B70-323[»]
C324-565[»]
D1-69[»]
ProteinModelPortaliA9X4C2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains RdRp catalytic domain.SAAS annotation
Contains SF3 helicase domain.SAAS annotation
Contains SFhelicase domain.SAAS annotation

Family and domain databases

Gene3Di2.60.120.20. 3 hits.
3.40.50.300. 1 hit.
4.10.80.10. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR014838. P3A.
IPR000081. Peptidase_C3.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR003138. Pico_P1A.
IPR002527. Pico_P2B.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
[Graphical view]
PfamiPF08727. P3A. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF02226. Pico_P1A. 1 hit.
PF00947. Pico_P2A. 1 hit.
PF01552. Pico_P2B. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 3 hits.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
ProDomiPD001306. Peptidase_C3. 1 hit.
PD649346. Pico_P2B. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF89043. SSF89043. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A9X4C2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSQVSTQRS GSHENSNSAT EGSTINYTTI NYYKDSYAAT AGKQSLKQDP
60 70 80 90 100
DKFANPVKDI FTEMAAPLKS PSAEACGYSD RVAQLTIGNS TITTQEAANI
110 120 130 140 150
IVGYGEWPSY CSDDDATAVD KPTRPDVSVN RFYTLDTKLW EKSSKGWYWK
160 170 180 190 200
FPDVLTETGV FGQNAQFHYL YRSGFCIHVQ CNASKFHQGA LLVAILPEYV
210 220 230 240 250
IGTVAGGTGT EDSHPPYKQT QPGADGFELQ HPYVLDAGIP ISQLTVCPHQ
260 270 280 290 300
WINLRTNNCA TIIVPYMNTL PFDSALNHCN FGLLVVPISP LDFDQGATPV
310 320 330 340 350
IPITITLAPM CSEFAGLRQA VTQGFPTEPK PGTNQFLTTD DGVSAPILPN
360 370 380 390 400
FHPTPCIHIP GEVRNLLELC QVETILEVNN VPTNATSLME RLRFPVSAQA
410 420 430 440 450
GKGELCAVFR ADPGRDGPWQ STMLGQLCGY YTQWSGSLEV TFMFTGSFMA
460 470 480 490 500
TGKMLIAYTP PGGPLPKDRA TAMLGTHVIW DFGLQSSVTL VIPWISNTHY
510 520 530 540 550
RAHARDGVFD YYTTGLVSIW YQTNYVVPIG APNTAYIIAL AAAQKNFTMK
560 570 580 590 600
LCKDTSHILQ TASIQGDRVA DVIESSIGDS VSRALTQALP APTGQNTQVS
610 620 630 640 650
SHRLDTGEVP ALQAAEIGAS SNTSDESMIE TRCVLNSHST AETTLDSFFS
660 670 680 690 700
RAGLVGEIDL PLEGTTNPNG YANWDIDITG YAQMRRKVEL FTYMRFDAEF
710 720 730 740 750
TFVACTPTGQ VVPQLLQYMF VPPGAPKPES RESLAWQTAT NPSVFVKLTD
760 770 780 790 800
PPAQVSVPFM SPASAYQWFY DGYPTFGEHK QEKDLEYGAC PNNMMGTFSV
810 820 830 840 850
RNVGSSKSKY PLVVRIYMRM KHVRAWIPRP MRNQNYLFKA NPNYAGNSIK
860 870 880 890 900
PTGTSRTAIT TLGKFGQQSG AIYVGNFRVV NRHLATHNDW ANLVWEDSSR
910 920 930 940 950
DLLVSSTTAQ GCDTIARCDC QTGVYYCNSK RKHYPVSFSK PSLIYVEASE
960 970 980 990 1000
YYPARYQSHL MLAAGHSEPG DCGGILRCQH GVVGIVSTGG NGLVGFADVR
1010 1020 1030 1040 1050
DLLWLDEEAM EQGVSDYIKG LGDAFGTGFT DAVSREVEAL KNHLIGSEGA
1060 1070 1080 1090 1100
VEKILKNLIK LISALVIVIR SDYDMVTLTA TLALIGCHGS PWAWIKAKTA
1110 1120 1130 1140 1150
SILGIPIAQK QSASWLKKFN DMANAAKGLE WISSKISKFI DWLKEKIIPA
1160 1170 1180 1190 1200
AREKVEFLNN LKQLPLLENQ ISNLEQSAAS QEDLEAMFGN VSYLAHFCRK
1210 1220 1230 1240 1250
FQPLYATEAK RVYALEKRMN NYMQFKSKHR IEPVCLIIRG SPGTGKSLAT
1260 1270 1280 1290 1300
GIIARAIADK YHSSVYSLPP DPDHFDGYKQ QVVTVMDDLC QNPDGKDMSL
1310 1320 1330 1340 1350
FCQMVSTVDF IPPMASLEEK GVSFTSKFVI ASTNSSNIIV PTVSDSDAIR
1360 1370 1380 1390 1400
RRFYMDCDIE VTDSYKTDLG RLDAGRAAKL CSENNTANFK RCSPLVCGKA
1410 1420 1430 1440 1450
IQLRDRKSKV RYSVDTVVSE LIREYNNRSA IGNTIEALFQ GPPKFRPIRI
1460 1470 1480 1490 1500
SLEEKPAPDA ISDLLASVDS EEVRQYCRDQ GWIIPETPTN VERHLNRAVL
1510 1520 1530 1540 1550
VMQSIATVVA VVSLVYVIYK LFAGFQGAYS GAPKQILKKP VLRTATVQGP
1560 1570 1580 1590 1600
SLDFALSLLR RNIRQVQTDQ GHFTMLGVRD RLAVLPRHSQ PGKTIWVEHK
1610 1620 1630 1640 1650
LVNILDAVEL VDEQGVNLEL TLITLDTNEK FRDITKFIPE SISAASDATL
1660 1670 1680 1690 1700
VINTEHMPSM FVPVGDVVQY GFLNLSGKPT HRTMMYNFPT KAGQCGGVVT
1710 1720 1730 1740 1750
SVGKVIGIHI GGNGRQGFCA GLKRSYFASE QGEIQWVKPN KETGRLNING
1760 1770 1780 1790 1800
PTRTKLEPSV FHDVFEGNKE PAVLHSKDPR LEVDFEQALF SKYVGNTLYE
1810 1820 1830 1840 1850
PDEYIKEAAL HYANQLKQLD IDTSQMSMEE ACYGTENLEA IDLHTSAGYP
1860 1870 1880 1890 1900
YSALGIKKRD ILDSTTRDVS KMKFYMDKYG LDLPYSTYVK DELRSIDKIK
1910 1920 1930 1940 1950
KGKSRLIEAS SLNDSVYLRM TFGHLYETFH ANPGTVTGSA VGCNPDTFWS
1960 1970 1980 1990 2000
KLPILLPGSL FAFDYSGYDA SLSPVWFRAL ELVLREIGYS EEAVSLVEGI
2010 2020 2030 2040 2050
NHTHHVYRNK TYCVLGGMPS GCSGTSIFNS MINNIIIRAL LIKTFKGIDL
2060 2070 2080 2090 2100
DELNMVAYGD DVLASYPFPI DCLELARTGK EYGLTMTPAD KSPCFNEVNW
2110 2120 2130 2140 2150
DNVTFLKRGF LPDEQFPFLI HPTMPMKEIH ESIRWTKDAR NTQDHVRSLC
2160 2170 2180 2190
LLAWHNGKQE YEKFVSAIRS VPVGKALAIP NYENLRRNWL ELF
Length:2,193
Mass (Da):242,982
Last modified:February 5, 2008 - v1
Checksum:iF07DD434AE01FB4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ341368 Genomic RNA. Translation: ABC69262.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ341368 Genomic RNA. Translation: ABC69262.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3ZFE X-ray 2.70 A 566-863 [» ]
B 70-323 [» ]
C 324-565 [» ]
D 1-69 [» ]
3ZFF X-ray 3.40 A 566-862 [» ]
B 70-323 [» ]
C 324-565 [» ]
D 1-69 [» ]
3ZFG X-ray 3.20 A 566-863 [» ]
B 70-323 [» ]
C 324-565 [» ]
D 1-69 [» ]
4AED X-ray 3.80 A 566-862 [» ]
B 70-323 [» ]
C 324-565 [» ]
D 1-69 [» ]
4C0U electron microscopy 10.00 A 566-863 [» ]
B 70-323 [» ]
C 324-565 [» ]
4C0Y electron microscopy 16.00 A 566-863 [» ]
B 70-323 [» ]
C 324-565 [» ]
4C10 electron microscopy 13.00 A 566-863 [» ]
B 70-323 [» ]
C 324-565 [» ]
D 1-69 [» ]
ProteinModelPortali A9X4C2.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.20. 3 hits.
3.40.50.300. 1 hit.
4.10.80.10. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR014838. P3A.
IPR000081. Peptidase_C3.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR003138. Pico_P1A.
IPR002527. Pico_P2B.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
[Graphical view ]
Pfami PF08727. P3A. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF02226. Pico_P1A. 1 hit.
PF00947. Pico_P2A. 1 hit.
PF01552. Pico_P2B. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 3 hits.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
ProDomi PD001306. Peptidase_C3. 1 hit.
PD649346. Pico_P2B. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF89043. SSF89043. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Full genome sequencing of human enterovirus type 71."
    Perera D., Apandi M., Cardosa M.J.
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: MY104-9-SAR-97Imported.
  2. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: MY104-9-SAR-97Imported.
  3. Cited for: X-RAY CRYSTALLOGRAPHY (3.80 ANGSTROMS) OF 566-862; 70-323; 324-565 AND 1-69 IN COMPLEX WITH CALCIUM.
  4. "Structure of human enterovirus 71 in complex with a capsid-binding inhibitor."
    Plevka P., Perera R., Yap M.L., Cardosa J., Kuhn R.J., Rossmann M.G.
    Proc. Natl. Acad. Sci. U.S.A. 110:5463-5467(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF 566-863; 70-323; 324-565 AND 1-69.
  5. "Neutralizing antibodies can initiate genome release from human enterovirus 71."
    Plevka P., Lim P.Y., Perera R., Cardosa J., Suksatu A., Kuhn R.J., Rossmann M.G.
    Proc. Natl. Acad. Sci. U.S.A. 111:2134-2139(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (10.00 ANGSTROMS) OF 566-863; 70-323 AND 324-565.

Entry informationi

Entry nameiA9X4C2_9ENTO
AccessioniPrimary (citable) accession number: A9X4C2
Entry historyi
Integrated into UniProtKB/TrEMBL: February 5, 2008
Last sequence update: February 5, 2008
Last modified: November 26, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3