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A9X4C2 (A9X4C2_9ENTO) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein names
OrganismEnterovirus A71 EMBL ABC69262.1
Taxonomic identifier39054 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesPicornaviridaeEnterovirusEnterovirus A

Protein attributes

Sequence length2193 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

NTP + H2O = NDP + phosphate. SAAS SAAS014759

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). SAAS SAAS014759

Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly. SAAS SAAS014759

Subcellular location

Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity SAAS SAAS014759.

Sequence similarities

Contains RdRp catalytic domain. SAAS SAAS014759

Contains SF3 helicase domain. SAAS SAAS014759

Contains SFhelicase domain. SAAS SAAS014759

Ontologies

Keywords
   Biological processHost-virus interaction
Ion transport
Transport
Viral attachment to host cell SAAS SAAS014759
Viral RNA replication SAAS SAAS014759
Virus entry into host cell
   Cellular componentCapsid protein SAAS SAAS014759
Host cytoplasm SAAS SAAS014759
Host cytoplasmic vesicle SAAS SAAS014759
Host membrane SAAS SAAS014759
Membrane
Virion
   LigandATP-binding
Calcium PDB 4AED
Metal-binding PDB 4C10 PDB 3ZFE PDB 4AED
Nucleotide-binding
RNA-binding SAAS SAAS014759
Sodium PDB 4C10 PDB 3ZFE
   Molecular functionHelicase SAAS SAAS014759
Hydrolase
Ion channel
Nucleotidyltransferase
Protease
RNA-directed RNA polymerase SAAS SAAS014759
Thiol protease SAAS SAAS014759
Transferase
Viral ion channel SAAS SAAS014759
   PTMPhosphoprotein SAAS SAAS014759
   Technical term3D-structure PDB 4C10 PDB 3ZFE PDB 3ZFF PDB 3ZFG PDB 4AED PDB 4C0U PDB 4C0Y
Gene Ontology (GO)
   Biological_processRNA-protein covalent cross-linking

Inferred from electronic annotation. Source: InterPro

pore formation by virus in membrane of host cell

Inferred from electronic annotation. Source: UniProtKB-KW

protein oligomerization

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

viral RNA genome replication

Inferred from electronic annotation. Source: InterPro

viral entry into host cell

Inferred from electronic annotation. Source: UniProtKB-KW

virion attachment to host cell

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell cytoplasmic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane of host cell

Inferred from electronic annotation. Source: UniProtKB-KW

membrane

Inferred from electronic annotation. Source: UniProtKB-KW

viral capsid

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA helicase activity

Inferred from electronic annotation. Source: InterPro

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

ion channel activity

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

structural molecule activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6969VP4 EMBL ABC69262.1
PRO_5000300266
Chain70 – 323254VP2 EMBL ABC69262.1
PRO_5000300267
Chain324 – 565242VP3 EMBL ABC69262.1
PRO_5000300268
Chain566 – 862297VP1 EMBL ABC69262.1
PRO_5000300269
Chain863 – 10121502A EMBL ABC69262.1
PRO_5000300270
Chain1013 – 1111992B EMBL ABC69262.1
PRO_5000300271
Chain1112 – 14403292C EMBL ABC69262.1
PRO_5000300272
Chain1441 – 1526863A EMBL ABC69262.1
PRO_5000300273
Chain1527 – 1548223B EMBL ABC69262.1
PRO_5000300274
Chain1549 – 17311833C EMBL ABC69262.1
PRO_5000300275
Chain1732 – 21934623D EMBL ABC69262.1
PRO_5000300276

Sites

Metal binding1361Calcium PDB 4AED
Metal binding2251Calcium PDB 4AED
Metal binding3281Sodium 1 PDB 4C10 PDB 3ZFE
Metal binding3311Sodium 1; via carbonyl oxygen PDB 4C10 PDB 3ZFE
Metal binding3431Sodium 1; via carbonyl oxygen PDB 4C10 PDB 3ZFE
Metal binding5931Sodium 2 PDB 4C10 PDB 3ZFE
Metal binding5941Sodium 2; via carbonyl oxygen PDB 4C10 PDB 3ZFE
Metal binding5961Sodium 2 PDB 4C10 PDB 3ZFE
Metal binding6361Sodium 2; via carbonyl oxygen PDB 4C10 PDB 3ZFE

Sequences

Sequence LengthMass (Da)Tools
A9X4C2 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: F07DD434AE01FB4A

FASTA2,193242,982
        10         20         30         40         50         60 
MGSQVSTQRS GSHENSNSAT EGSTINYTTI NYYKDSYAAT AGKQSLKQDP DKFANPVKDI 

        70         80         90        100        110        120 
FTEMAAPLKS PSAEACGYSD RVAQLTIGNS TITTQEAANI IVGYGEWPSY CSDDDATAVD 

       130        140        150        160        170        180 
KPTRPDVSVN RFYTLDTKLW EKSSKGWYWK FPDVLTETGV FGQNAQFHYL YRSGFCIHVQ 

       190        200        210        220        230        240 
CNASKFHQGA LLVAILPEYV IGTVAGGTGT EDSHPPYKQT QPGADGFELQ HPYVLDAGIP 

       250        260        270        280        290        300 
ISQLTVCPHQ WINLRTNNCA TIIVPYMNTL PFDSALNHCN FGLLVVPISP LDFDQGATPV 

       310        320        330        340        350        360 
IPITITLAPM CSEFAGLRQA VTQGFPTEPK PGTNQFLTTD DGVSAPILPN FHPTPCIHIP 

       370        380        390        400        410        420 
GEVRNLLELC QVETILEVNN VPTNATSLME RLRFPVSAQA GKGELCAVFR ADPGRDGPWQ 

       430        440        450        460        470        480 
STMLGQLCGY YTQWSGSLEV TFMFTGSFMA TGKMLIAYTP PGGPLPKDRA TAMLGTHVIW 

       490        500        510        520        530        540 
DFGLQSSVTL VIPWISNTHY RAHARDGVFD YYTTGLVSIW YQTNYVVPIG APNTAYIIAL 

       550        560        570        580        590        600 
AAAQKNFTMK LCKDTSHILQ TASIQGDRVA DVIESSIGDS VSRALTQALP APTGQNTQVS 

       610        620        630        640        650        660 
SHRLDTGEVP ALQAAEIGAS SNTSDESMIE TRCVLNSHST AETTLDSFFS RAGLVGEIDL 

       670        680        690        700        710        720 
PLEGTTNPNG YANWDIDITG YAQMRRKVEL FTYMRFDAEF TFVACTPTGQ VVPQLLQYMF 

       730        740        750        760        770        780 
VPPGAPKPES RESLAWQTAT NPSVFVKLTD PPAQVSVPFM SPASAYQWFY DGYPTFGEHK 

       790        800        810        820        830        840 
QEKDLEYGAC PNNMMGTFSV RNVGSSKSKY PLVVRIYMRM KHVRAWIPRP MRNQNYLFKA 

       850        860        870        880        890        900 
NPNYAGNSIK PTGTSRTAIT TLGKFGQQSG AIYVGNFRVV NRHLATHNDW ANLVWEDSSR 

       910        920        930        940        950        960 
DLLVSSTTAQ GCDTIARCDC QTGVYYCNSK RKHYPVSFSK PSLIYVEASE YYPARYQSHL 

       970        980        990       1000       1010       1020 
MLAAGHSEPG DCGGILRCQH GVVGIVSTGG NGLVGFADVR DLLWLDEEAM EQGVSDYIKG 

      1030       1040       1050       1060       1070       1080 
LGDAFGTGFT DAVSREVEAL KNHLIGSEGA VEKILKNLIK LISALVIVIR SDYDMVTLTA 

      1090       1100       1110       1120       1130       1140 
TLALIGCHGS PWAWIKAKTA SILGIPIAQK QSASWLKKFN DMANAAKGLE WISSKISKFI 

      1150       1160       1170       1180       1190       1200 
DWLKEKIIPA AREKVEFLNN LKQLPLLENQ ISNLEQSAAS QEDLEAMFGN VSYLAHFCRK 

      1210       1220       1230       1240       1250       1260 
FQPLYATEAK RVYALEKRMN NYMQFKSKHR IEPVCLIIRG SPGTGKSLAT GIIARAIADK 

      1270       1280       1290       1300       1310       1320 
YHSSVYSLPP DPDHFDGYKQ QVVTVMDDLC QNPDGKDMSL FCQMVSTVDF IPPMASLEEK 

      1330       1340       1350       1360       1370       1380 
GVSFTSKFVI ASTNSSNIIV PTVSDSDAIR RRFYMDCDIE VTDSYKTDLG RLDAGRAAKL 

      1390       1400       1410       1420       1430       1440 
CSENNTANFK RCSPLVCGKA IQLRDRKSKV RYSVDTVVSE LIREYNNRSA IGNTIEALFQ 

      1450       1460       1470       1480       1490       1500 
GPPKFRPIRI SLEEKPAPDA ISDLLASVDS EEVRQYCRDQ GWIIPETPTN VERHLNRAVL 

      1510       1520       1530       1540       1550       1560 
VMQSIATVVA VVSLVYVIYK LFAGFQGAYS GAPKQILKKP VLRTATVQGP SLDFALSLLR 

      1570       1580       1590       1600       1610       1620 
RNIRQVQTDQ GHFTMLGVRD RLAVLPRHSQ PGKTIWVEHK LVNILDAVEL VDEQGVNLEL 

      1630       1640       1650       1660       1670       1680 
TLITLDTNEK FRDITKFIPE SISAASDATL VINTEHMPSM FVPVGDVVQY GFLNLSGKPT 

      1690       1700       1710       1720       1730       1740 
HRTMMYNFPT KAGQCGGVVT SVGKVIGIHI GGNGRQGFCA GLKRSYFASE QGEIQWVKPN 

      1750       1760       1770       1780       1790       1800 
KETGRLNING PTRTKLEPSV FHDVFEGNKE PAVLHSKDPR LEVDFEQALF SKYVGNTLYE 

      1810       1820       1830       1840       1850       1860 
PDEYIKEAAL HYANQLKQLD IDTSQMSMEE ACYGTENLEA IDLHTSAGYP YSALGIKKRD 

      1870       1880       1890       1900       1910       1920 
ILDSTTRDVS KMKFYMDKYG LDLPYSTYVK DELRSIDKIK KGKSRLIEAS SLNDSVYLRM 

      1930       1940       1950       1960       1970       1980 
TFGHLYETFH ANPGTVTGSA VGCNPDTFWS KLPILLPGSL FAFDYSGYDA SLSPVWFRAL 

      1990       2000       2010       2020       2030       2040 
ELVLREIGYS EEAVSLVEGI NHTHHVYRNK TYCVLGGMPS GCSGTSIFNS MINNIIIRAL 

      2050       2060       2070       2080       2090       2100 
LIKTFKGIDL DELNMVAYGD DVLASYPFPI DCLELARTGK EYGLTMTPAD KSPCFNEVNW 

      2110       2120       2130       2140       2150       2160 
DNVTFLKRGF LPDEQFPFLI HPTMPMKEIH ESIRWTKDAR NTQDHVRSLC LLAWHNGKQE 

      2170       2180       2190 
YEKFVSAIRS VPVGKALAIP NYENLRRNWL ELF 

« Hide

References

[1]"Full genome sequencing of human enterovirus type 71."
Perera D., Apandi M., Cardosa M.J.
Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: MY104-9-SAR-97 EMBL ABC69262.1.
[2]"Crystal structure of human enterovirus 71."
Plevka P., Perera R., Cardosa J., Kuhn R.J., Rossmann M.G.
Science 336:1274-1274(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.80 ANGSTROMS) OF 566-862; 70-323; 324-565 AND 1-69 IN COMPLEX WITH CALCIUM.
[3]"Structure of human enterovirus 71 in complex with a capsid-binding inhibitor."
Plevka P., Perera R., Yap M.L., Cardosa J., Kuhn R.J., Rossmann M.G.
Proc. Natl. Acad. Sci. U.S.A. 110:5463-5467(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF 566-863; 70-323; 324-565 AND 1-69 IN COMPLEX WITH SODIUM.
[4]"Neutralizing antibodies can initiate genome release from human enterovirus 71."
Plevka P., Lim P.Y., Perera R., Cardosa J., Suksatu A., Kuhn R.J., Rossmann M.G.
Proc. Natl. Acad. Sci. U.S.A. 111:2134-2139(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (10.00 ANGSTROMS) OF 566-863; 70-323 AND 324-565 IN COMPLEX WITH SODIUM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ341368 Genomic RNA. Translation: ABC69262.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZFEX-ray2.70A566-863[»]
B70-323[»]
C324-565[»]
D1-69[»]
3ZFFX-ray3.40A566-862[»]
B70-323[»]
C324-565[»]
D1-69[»]
3ZFGX-ray3.20A566-863[»]
B70-323[»]
C324-565[»]
D1-69[»]
4AEDX-ray3.80A566-862[»]
B70-323[»]
C324-565[»]
D1-69[»]
4C0Uelectron microscopy10.00A566-863[»]
B70-323[»]
C324-565[»]
4C0Yelectron microscopy16.00A566-863[»]
B70-323[»]
C324-565[»]
4C10electron microscopy13.00A566-863[»]
B70-323[»]
C324-565[»]
D1-69[»]
ProteinModelPortalA9X4C2.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.300. 1 hit.
4.10.80.10. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR014838. P3A.
IPR000081. Peptidase_C3.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR003138. Pico_P1A.
IPR002527. Pico_P2B.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF08727. P3A. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF02226. Pico_P1A. 1 hit.
PF00947. Pico_P2A. 1 hit.
PF01552. Pico_P2B. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 3 hits.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
ProDomPD001306. Peptidase_C3. 1 hit.
PD649346. Pico_P2B. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF89043. SSF89043. 1 hit.
PROSITEPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA9X4C2_9ENTO
AccessionPrimary (citable) accession number: A9X4C2
Entry history
Integrated into UniProtKB/TrEMBL: February 5, 2008
Last sequence update: February 5, 2008
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)