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A9X4C2

- A9X4C2_9ENTO

UniProt

A9X4C2 - A9X4C2_9ENTO

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Protein
Submitted name:

Polyprotein

Gene
N/A
Organism
Enterovirus A71
Status
Unreviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

NTP + H2O = NDP + phosphate.SAAS annotations
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SAAS annotations
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.SAAS annotations

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi136 – 1361CalciumImported
Metal bindingi225 – 2251CalciumImported
Metal bindingi328 – 3281Sodium 1Imported
Metal bindingi331 – 3311Sodium 1; via carbonyl oxygenImported
Metal bindingi343 – 3431Sodium 1; via carbonyl oxygenImported
Metal bindingi593 – 5931Sodium 2Imported
Metal bindingi594 – 5941Sodium 2; via carbonyl oxygenImported
Metal bindingi596 – 5961Sodium 2Imported
Metal bindingi636 – 6361Sodium 2; via carbonyl oxygenImported

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. ion channel activity Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW
  5. RNA binding Source: UniProtKB-KW
  6. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  7. RNA helicase activity Source: InterPro
  8. structural molecule activity Source: InterPro

GO - Biological processi

  1. pore formation by virus in membrane of host cell Source: UniProtKB-KW
  2. protein oligomerization Source: UniProtKB-KW
  3. RNA-protein covalent cross-linking Source: InterPro
  4. transcription, DNA-templated Source: InterPro
  5. viral entry into host cell Source: UniProtKB-KW
  6. viral RNA genome replication Source: InterPro
  7. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

HelicaseSAAS annotations, Hydrolase, Ion channel, Nucleotidyltransferase, Protease, RNA-directed RNA polymeraseSAAS annotations, Thiol proteaseSAAS annotations, Transferase, Viral ion channelSAAS annotations

Keywords - Biological processi

Host-virus interaction, Ion transport, Transport, Viral attachment to host cellSAAS annotations, Viral RNA replicationSAAS annotations, Virus entry into host cell

Keywords - Ligandi

ATP-binding, CalciumImported, Metal-bindingImported, Nucleotide-binding, RNA-bindingSAAS annotations, SodiumImported

Names & Taxonomyi

Protein namesi
Submitted name:
PolyproteinImported
OrganismiEnterovirus A71Imported
Taxonomic identifieri39054 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesPicornaviridaeEnterovirusEnterovirus A

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  2. integral to membrane of host cell Source: UniProtKB-KW
  3. membrane Source: UniProtKB-KW
  4. viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid proteinSAAS annotations, Host cytoplasmSAAS annotations, Host cytoplasmic vesicleSAAS annotations, Host membraneSAAS annotations, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6969VP4ImportedPRO_5000300266Add
BLAST
Chaini70 – 323254VP2ImportedPRO_5000300267Add
BLAST
Chaini324 – 565242VP3ImportedPRO_5000300268Add
BLAST
Chaini566 – 862297VP1ImportedPRO_5000300269Add
BLAST
Chaini863 – 10121502AImportedPRO_5000300270Add
BLAST
Chaini1013 – 1111992BImportedPRO_5000300271Add
BLAST
Chaini1112 – 14403292CImportedPRO_5000300272Add
BLAST
Chaini1441 – 1526863AImportedPRO_5000300273Add
BLAST
Chaini1527 – 1548223BImportedPRO_5000300274Add
BLAST
Chaini1549 – 17311833CImportedPRO_5000300275Add
BLAST
Chaini1732 – 21934623DImportedPRO_5000300276Add
BLAST

Keywords - PTMi

PhosphoproteinSAAS annotations

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZFEX-ray2.70A566-863[»]
B70-323[»]
C324-565[»]
D1-69[»]
3ZFFX-ray3.40A566-862[»]
B70-323[»]
C324-565[»]
D1-69[»]
3ZFGX-ray3.20A566-863[»]
B70-323[»]
C324-565[»]
D1-69[»]
4AEDX-ray3.80A566-862[»]
B70-323[»]
C324-565[»]
D1-69[»]
4C0Uelectron microscopy10.00A566-863[»]
B70-323[»]
C324-565[»]
4C0Yelectron microscopy16.00A566-863[»]
B70-323[»]
C324-565[»]
4C10electron microscopy13.00A566-863[»]
B70-323[»]
C324-565[»]
D1-69[»]
ProteinModelPortaliA9X4C2.

Family & Domainsi

Sequence similaritiesi

Contains RdRp catalytic domain.SAAS annotations
Contains SF3 helicase domain.SAAS annotations
Contains SFhelicase domain.SAAS annotations

Family and domain databases

Gene3Di2.60.120.20. 3 hits.
3.40.50.300. 1 hit.
4.10.80.10. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR014838. P3A.
IPR000081. Peptidase_C3.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR003138. Pico_P1A.
IPR002527. Pico_P2B.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
[Graphical view]
PfamiPF08727. P3A. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF02226. Pico_P1A. 1 hit.
PF00947. Pico_P2A. 1 hit.
PF01552. Pico_P2B. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 3 hits.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
ProDomiPD001306. Peptidase_C3. 1 hit.
PD649346. Pico_P2B. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF89043. SSF89043. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9X4C2-1 [UniParc]FASTAAdd to Basket

« Hide

MGSQVSTQRS GSHENSNSAT EGSTINYTTI NYYKDSYAAT AGKQSLKQDP     50
DKFANPVKDI FTEMAAPLKS PSAEACGYSD RVAQLTIGNS TITTQEAANI 100
IVGYGEWPSY CSDDDATAVD KPTRPDVSVN RFYTLDTKLW EKSSKGWYWK 150
FPDVLTETGV FGQNAQFHYL YRSGFCIHVQ CNASKFHQGA LLVAILPEYV 200
IGTVAGGTGT EDSHPPYKQT QPGADGFELQ HPYVLDAGIP ISQLTVCPHQ 250
WINLRTNNCA TIIVPYMNTL PFDSALNHCN FGLLVVPISP LDFDQGATPV 300
IPITITLAPM CSEFAGLRQA VTQGFPTEPK PGTNQFLTTD DGVSAPILPN 350
FHPTPCIHIP GEVRNLLELC QVETILEVNN VPTNATSLME RLRFPVSAQA 400
GKGELCAVFR ADPGRDGPWQ STMLGQLCGY YTQWSGSLEV TFMFTGSFMA 450
TGKMLIAYTP PGGPLPKDRA TAMLGTHVIW DFGLQSSVTL VIPWISNTHY 500
RAHARDGVFD YYTTGLVSIW YQTNYVVPIG APNTAYIIAL AAAQKNFTMK 550
LCKDTSHILQ TASIQGDRVA DVIESSIGDS VSRALTQALP APTGQNTQVS 600
SHRLDTGEVP ALQAAEIGAS SNTSDESMIE TRCVLNSHST AETTLDSFFS 650
RAGLVGEIDL PLEGTTNPNG YANWDIDITG YAQMRRKVEL FTYMRFDAEF 700
TFVACTPTGQ VVPQLLQYMF VPPGAPKPES RESLAWQTAT NPSVFVKLTD 750
PPAQVSVPFM SPASAYQWFY DGYPTFGEHK QEKDLEYGAC PNNMMGTFSV 800
RNVGSSKSKY PLVVRIYMRM KHVRAWIPRP MRNQNYLFKA NPNYAGNSIK 850
PTGTSRTAIT TLGKFGQQSG AIYVGNFRVV NRHLATHNDW ANLVWEDSSR 900
DLLVSSTTAQ GCDTIARCDC QTGVYYCNSK RKHYPVSFSK PSLIYVEASE 950
YYPARYQSHL MLAAGHSEPG DCGGILRCQH GVVGIVSTGG NGLVGFADVR 1000
DLLWLDEEAM EQGVSDYIKG LGDAFGTGFT DAVSREVEAL KNHLIGSEGA 1050
VEKILKNLIK LISALVIVIR SDYDMVTLTA TLALIGCHGS PWAWIKAKTA 1100
SILGIPIAQK QSASWLKKFN DMANAAKGLE WISSKISKFI DWLKEKIIPA 1150
AREKVEFLNN LKQLPLLENQ ISNLEQSAAS QEDLEAMFGN VSYLAHFCRK 1200
FQPLYATEAK RVYALEKRMN NYMQFKSKHR IEPVCLIIRG SPGTGKSLAT 1250
GIIARAIADK YHSSVYSLPP DPDHFDGYKQ QVVTVMDDLC QNPDGKDMSL 1300
FCQMVSTVDF IPPMASLEEK GVSFTSKFVI ASTNSSNIIV PTVSDSDAIR 1350
RRFYMDCDIE VTDSYKTDLG RLDAGRAAKL CSENNTANFK RCSPLVCGKA 1400
IQLRDRKSKV RYSVDTVVSE LIREYNNRSA IGNTIEALFQ GPPKFRPIRI 1450
SLEEKPAPDA ISDLLASVDS EEVRQYCRDQ GWIIPETPTN VERHLNRAVL 1500
VMQSIATVVA VVSLVYVIYK LFAGFQGAYS GAPKQILKKP VLRTATVQGP 1550
SLDFALSLLR RNIRQVQTDQ GHFTMLGVRD RLAVLPRHSQ PGKTIWVEHK 1600
LVNILDAVEL VDEQGVNLEL TLITLDTNEK FRDITKFIPE SISAASDATL 1650
VINTEHMPSM FVPVGDVVQY GFLNLSGKPT HRTMMYNFPT KAGQCGGVVT 1700
SVGKVIGIHI GGNGRQGFCA GLKRSYFASE QGEIQWVKPN KETGRLNING 1750
PTRTKLEPSV FHDVFEGNKE PAVLHSKDPR LEVDFEQALF SKYVGNTLYE 1800
PDEYIKEAAL HYANQLKQLD IDTSQMSMEE ACYGTENLEA IDLHTSAGYP 1850
YSALGIKKRD ILDSTTRDVS KMKFYMDKYG LDLPYSTYVK DELRSIDKIK 1900
KGKSRLIEAS SLNDSVYLRM TFGHLYETFH ANPGTVTGSA VGCNPDTFWS 1950
KLPILLPGSL FAFDYSGYDA SLSPVWFRAL ELVLREIGYS EEAVSLVEGI 2000
NHTHHVYRNK TYCVLGGMPS GCSGTSIFNS MINNIIIRAL LIKTFKGIDL 2050
DELNMVAYGD DVLASYPFPI DCLELARTGK EYGLTMTPAD KSPCFNEVNW 2100
DNVTFLKRGF LPDEQFPFLI HPTMPMKEIH ESIRWTKDAR NTQDHVRSLC 2150
LLAWHNGKQE YEKFVSAIRS VPVGKALAIP NYENLRRNWL ELF 2193
Length:2,193
Mass (Da):242,982
Last modified:February 5, 2008 - v1
Checksum:iF07DD434AE01FB4A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ341368 Genomic RNA. Translation: ABC69262.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ341368 Genomic RNA. Translation: ABC69262.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3ZFE X-ray 2.70 A 566-863 [» ]
B 70-323 [» ]
C 324-565 [» ]
D 1-69 [» ]
3ZFF X-ray 3.40 A 566-862 [» ]
B 70-323 [» ]
C 324-565 [» ]
D 1-69 [» ]
3ZFG X-ray 3.20 A 566-863 [» ]
B 70-323 [» ]
C 324-565 [» ]
D 1-69 [» ]
4AED X-ray 3.80 A 566-862 [» ]
B 70-323 [» ]
C 324-565 [» ]
D 1-69 [» ]
4C0U electron microscopy 10.00 A 566-863 [» ]
B 70-323 [» ]
C 324-565 [» ]
4C0Y electron microscopy 16.00 A 566-863 [» ]
B 70-323 [» ]
C 324-565 [» ]
4C10 electron microscopy 13.00 A 566-863 [» ]
B 70-323 [» ]
C 324-565 [» ]
D 1-69 [» ]
ProteinModelPortali A9X4C2.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.20. 3 hits.
3.40.50.300. 1 hit.
4.10.80.10. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR014838. P3A.
IPR000081. Peptidase_C3.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR003138. Pico_P1A.
IPR002527. Pico_P2B.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
[Graphical view ]
Pfami PF08727. P3A. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF02226. Pico_P1A. 1 hit.
PF00947. Pico_P2A. 1 hit.
PF01552. Pico_P2B. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 3 hits.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
ProDomi PD001306. Peptidase_C3. 1 hit.
PD649346. Pico_P2B. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF89043. SSF89043. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Full genome sequencing of human enterovirus type 71."
    Perera D., Apandi M., Cardosa M.J.
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: MY104-9-SAR-97Imported.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (3.80 ANGSTROMS) OF 566-862; 70-323; 324-565 AND 1-69 IN COMPLEX WITH CALCIUM.
  3. "Structure of human enterovirus 71 in complex with a capsid-binding inhibitor."
    Plevka P., Perera R., Yap M.L., Cardosa J., Kuhn R.J., Rossmann M.G.
    Proc. Natl. Acad. Sci. U.S.A. 110:5463-5467(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF 566-863; 70-323; 324-565 AND 1-69 IN COMPLEX WITH SODIUM.
  4. "Neutralizing antibodies can initiate genome release from human enterovirus 71."
    Plevka P., Lim P.Y., Perera R., Cardosa J., Suksatu A., Kuhn R.J., Rossmann M.G.
    Proc. Natl. Acad. Sci. U.S.A. 111:2134-2139(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (10.00 ANGSTROMS) OF 566-863; 70-323 AND 324-565 IN COMPLEX WITH SODIUM.

Entry informationi

Entry nameiA9X4C2_9ENTO
AccessioniPrimary (citable) accession number: A9X4C2
Entry historyi
Integrated into UniProtKB/TrEMBL: February 5, 2008
Last sequence update: February 5, 2008
Last modified: September 3, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3

Similar proteinsi