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A9X4C2

- A9X4C2_9ENTO

UniProt

A9X4C2 - A9X4C2_9ENTO

Protein
Submitted name:

Polyprotein

Gene
N/A
Organism
Enterovirus A71
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    NTP + H2O = NDP + phosphate.SAAS annotation
    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SAAS annotation
    Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.SAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi136 – 1361CalciumImported
    Metal bindingi225 – 2251CalciumImported

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. ion channel activity Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW
    5. RNA binding Source: UniProtKB-KW
    6. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    7. RNA helicase activity Source: InterPro
    8. structural molecule activity Source: InterPro

    GO - Biological processi

    1. pore formation by virus in membrane of host cell Source: UniProtKB-KW
    2. protein oligomerization Source: UniProtKB-KW
    3. RNA-protein covalent cross-linking Source: InterPro
    4. transcription, DNA-templated Source: InterPro
    5. viral entry into host cell Source: UniProtKB-KW
    6. viral RNA genome replication Source: InterPro
    7. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    HelicaseSAAS annotation, Hydrolase, Ion channel, Nucleotidyltransferase, Protease, RNA-directed RNA polymeraseSAAS annotation, Thiol proteaseSAAS annotation, Transferase, Viral ion channelSAAS annotation

    Keywords - Biological processi

    Host-virus interaction, Ion transport, Transport, Viral attachment to host cellSAAS annotation, Viral RNA replicationSAAS annotation, Virus entry into host cell

    Keywords - Ligandi

    ATP-binding, CalciumImported, Metal-bindingImported, Nucleotide-binding, RNA-bindingSAAS annotation

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    PolyproteinImported
    OrganismiEnterovirus A71Imported
    Taxonomic identifieri39054 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesPicornaviridaeEnterovirusEnterovirus A

    Subcellular locationi

    Host cytoplasm SAAS annotation
    Host cytoplasmic vesicle membrane SAAS annotation; Peripheral membrane protein SAAS annotation; Cytoplasmic side SAAS annotation

    GO - Cellular componenti

    1. host cell cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    2. integral to membrane of host cell Source: UniProtKB-KW
    3. membrane Source: UniProtKB-KW
    4. viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid proteinSAAS annotation, Host cytoplasmSAAS annotation, Host cytoplasmic vesicleSAAS annotation, Host membraneSAAS annotation, Membrane, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6969VP4ImportedPRO_5000300266Add
    BLAST
    Chaini70 – 323254VP2ImportedPRO_5000300267Add
    BLAST
    Chaini324 – 565242VP3ImportedPRO_5000300268Add
    BLAST
    Chaini566 – 862297VP1ImportedPRO_5000300269Add
    BLAST
    Chaini863 – 10121502AImportedPRO_5000300270Add
    BLAST
    Chaini1013 – 1111992BImportedPRO_5000300271Add
    BLAST
    Chaini1112 – 14403292CImportedPRO_5000300272Add
    BLAST
    Chaini1441 – 1526863AImportedPRO_5000300273Add
    BLAST
    Chaini1527 – 1548223BImportedPRO_5000300274Add
    BLAST
    Chaini1549 – 17311833CImportedPRO_5000300275Add
    BLAST
    Chaini1732 – 21934623DImportedPRO_5000300276Add
    BLAST

    Keywords - PTMi

    PhosphoproteinSAAS annotation

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3ZFEX-ray2.70A566-863[»]
    B70-323[»]
    C324-565[»]
    D1-69[»]
    3ZFFX-ray3.40A566-862[»]
    B70-323[»]
    C324-565[»]
    D1-69[»]
    3ZFGX-ray3.20A566-863[»]
    B70-323[»]
    C324-565[»]
    D1-69[»]
    4AEDX-ray3.80A566-862[»]
    B70-323[»]
    C324-565[»]
    D1-69[»]
    4C0Uelectron microscopy10.00A566-863[»]
    B70-323[»]
    C324-565[»]
    4C0Yelectron microscopy16.00A566-863[»]
    B70-323[»]
    C324-565[»]
    4C10electron microscopy13.00A566-863[»]
    B70-323[»]
    C324-565[»]
    D1-69[»]
    ProteinModelPortaliA9X4C2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains RdRp catalytic domain.SAAS annotation
    Contains SF3 helicase domain.SAAS annotation
    Contains SFhelicase domain.SAAS annotation

    Family and domain databases

    Gene3Di2.60.120.20. 3 hits.
    3.40.50.300. 1 hit.
    4.10.80.10. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR014838. P3A.
    IPR000081. Peptidase_C3.
    IPR000199. Peptidase_C3A/C3B_picornavir.
    IPR003138. Pico_P1A.
    IPR002527. Pico_P2B.
    IPR001676. Picornavirus_capsid.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view]
    PfamiPF08727. P3A. 1 hit.
    PF00548. Peptidase_C3. 1 hit.
    PF02226. Pico_P1A. 1 hit.
    PF00947. Pico_P2A. 1 hit.
    PF01552. Pico_P2B. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00073. Rhv. 3 hits.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view]
    ProDomiPD001306. Peptidase_C3. 1 hit.
    PD649346. Pico_P2B. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF89043. SSF89043. 1 hit.
    PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A9X4C2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSQVSTQRS GSHENSNSAT EGSTINYTTI NYYKDSYAAT AGKQSLKQDP     50
    DKFANPVKDI FTEMAAPLKS PSAEACGYSD RVAQLTIGNS TITTQEAANI 100
    IVGYGEWPSY CSDDDATAVD KPTRPDVSVN RFYTLDTKLW EKSSKGWYWK 150
    FPDVLTETGV FGQNAQFHYL YRSGFCIHVQ CNASKFHQGA LLVAILPEYV 200
    IGTVAGGTGT EDSHPPYKQT QPGADGFELQ HPYVLDAGIP ISQLTVCPHQ 250
    WINLRTNNCA TIIVPYMNTL PFDSALNHCN FGLLVVPISP LDFDQGATPV 300
    IPITITLAPM CSEFAGLRQA VTQGFPTEPK PGTNQFLTTD DGVSAPILPN 350
    FHPTPCIHIP GEVRNLLELC QVETILEVNN VPTNATSLME RLRFPVSAQA 400
    GKGELCAVFR ADPGRDGPWQ STMLGQLCGY YTQWSGSLEV TFMFTGSFMA 450
    TGKMLIAYTP PGGPLPKDRA TAMLGTHVIW DFGLQSSVTL VIPWISNTHY 500
    RAHARDGVFD YYTTGLVSIW YQTNYVVPIG APNTAYIIAL AAAQKNFTMK 550
    LCKDTSHILQ TASIQGDRVA DVIESSIGDS VSRALTQALP APTGQNTQVS 600
    SHRLDTGEVP ALQAAEIGAS SNTSDESMIE TRCVLNSHST AETTLDSFFS 650
    RAGLVGEIDL PLEGTTNPNG YANWDIDITG YAQMRRKVEL FTYMRFDAEF 700
    TFVACTPTGQ VVPQLLQYMF VPPGAPKPES RESLAWQTAT NPSVFVKLTD 750
    PPAQVSVPFM SPASAYQWFY DGYPTFGEHK QEKDLEYGAC PNNMMGTFSV 800
    RNVGSSKSKY PLVVRIYMRM KHVRAWIPRP MRNQNYLFKA NPNYAGNSIK 850
    PTGTSRTAIT TLGKFGQQSG AIYVGNFRVV NRHLATHNDW ANLVWEDSSR 900
    DLLVSSTTAQ GCDTIARCDC QTGVYYCNSK RKHYPVSFSK PSLIYVEASE 950
    YYPARYQSHL MLAAGHSEPG DCGGILRCQH GVVGIVSTGG NGLVGFADVR 1000
    DLLWLDEEAM EQGVSDYIKG LGDAFGTGFT DAVSREVEAL KNHLIGSEGA 1050
    VEKILKNLIK LISALVIVIR SDYDMVTLTA TLALIGCHGS PWAWIKAKTA 1100
    SILGIPIAQK QSASWLKKFN DMANAAKGLE WISSKISKFI DWLKEKIIPA 1150
    AREKVEFLNN LKQLPLLENQ ISNLEQSAAS QEDLEAMFGN VSYLAHFCRK 1200
    FQPLYATEAK RVYALEKRMN NYMQFKSKHR IEPVCLIIRG SPGTGKSLAT 1250
    GIIARAIADK YHSSVYSLPP DPDHFDGYKQ QVVTVMDDLC QNPDGKDMSL 1300
    FCQMVSTVDF IPPMASLEEK GVSFTSKFVI ASTNSSNIIV PTVSDSDAIR 1350
    RRFYMDCDIE VTDSYKTDLG RLDAGRAAKL CSENNTANFK RCSPLVCGKA 1400
    IQLRDRKSKV RYSVDTVVSE LIREYNNRSA IGNTIEALFQ GPPKFRPIRI 1450
    SLEEKPAPDA ISDLLASVDS EEVRQYCRDQ GWIIPETPTN VERHLNRAVL 1500
    VMQSIATVVA VVSLVYVIYK LFAGFQGAYS GAPKQILKKP VLRTATVQGP 1550
    SLDFALSLLR RNIRQVQTDQ GHFTMLGVRD RLAVLPRHSQ PGKTIWVEHK 1600
    LVNILDAVEL VDEQGVNLEL TLITLDTNEK FRDITKFIPE SISAASDATL 1650
    VINTEHMPSM FVPVGDVVQY GFLNLSGKPT HRTMMYNFPT KAGQCGGVVT 1700
    SVGKVIGIHI GGNGRQGFCA GLKRSYFASE QGEIQWVKPN KETGRLNING 1750
    PTRTKLEPSV FHDVFEGNKE PAVLHSKDPR LEVDFEQALF SKYVGNTLYE 1800
    PDEYIKEAAL HYANQLKQLD IDTSQMSMEE ACYGTENLEA IDLHTSAGYP 1850
    YSALGIKKRD ILDSTTRDVS KMKFYMDKYG LDLPYSTYVK DELRSIDKIK 1900
    KGKSRLIEAS SLNDSVYLRM TFGHLYETFH ANPGTVTGSA VGCNPDTFWS 1950
    KLPILLPGSL FAFDYSGYDA SLSPVWFRAL ELVLREIGYS EEAVSLVEGI 2000
    NHTHHVYRNK TYCVLGGMPS GCSGTSIFNS MINNIIIRAL LIKTFKGIDL 2050
    DELNMVAYGD DVLASYPFPI DCLELARTGK EYGLTMTPAD KSPCFNEVNW 2100
    DNVTFLKRGF LPDEQFPFLI HPTMPMKEIH ESIRWTKDAR NTQDHVRSLC 2150
    LLAWHNGKQE YEKFVSAIRS VPVGKALAIP NYENLRRNWL ELF 2193
    Length:2,193
    Mass (Da):242,982
    Last modified:February 5, 2008 - v1
    Checksum:iF07DD434AE01FB4A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ341368 Genomic RNA. Translation: ABC69262.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ341368 Genomic RNA. Translation: ABC69262.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3ZFE X-ray 2.70 A 566-863 [» ]
    B 70-323 [» ]
    C 324-565 [» ]
    D 1-69 [» ]
    3ZFF X-ray 3.40 A 566-862 [» ]
    B 70-323 [» ]
    C 324-565 [» ]
    D 1-69 [» ]
    3ZFG X-ray 3.20 A 566-863 [» ]
    B 70-323 [» ]
    C 324-565 [» ]
    D 1-69 [» ]
    4AED X-ray 3.80 A 566-862 [» ]
    B 70-323 [» ]
    C 324-565 [» ]
    D 1-69 [» ]
    4C0U electron microscopy 10.00 A 566-863 [» ]
    B 70-323 [» ]
    C 324-565 [» ]
    4C0Y electron microscopy 16.00 A 566-863 [» ]
    B 70-323 [» ]
    C 324-565 [» ]
    4C10 electron microscopy 13.00 A 566-863 [» ]
    B 70-323 [» ]
    C 324-565 [» ]
    D 1-69 [» ]
    ProteinModelPortali A9X4C2.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.20. 3 hits.
    3.40.50.300. 1 hit.
    4.10.80.10. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR014838. P3A.
    IPR000081. Peptidase_C3.
    IPR000199. Peptidase_C3A/C3B_picornavir.
    IPR003138. Pico_P1A.
    IPR002527. Pico_P2B.
    IPR001676. Picornavirus_capsid.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view ]
    Pfami PF08727. P3A. 1 hit.
    PF00548. Peptidase_C3. 1 hit.
    PF02226. Pico_P1A. 1 hit.
    PF00947. Pico_P2A. 1 hit.
    PF01552. Pico_P2B. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00073. Rhv. 3 hits.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view ]
    ProDomi PD001306. Peptidase_C3. 1 hit.
    PD649346. Pico_P2B. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF89043. SSF89043. 1 hit.
    PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Full genome sequencing of human enterovirus type 71."
      Perera D., Apandi M., Cardosa M.J.
      Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: MY104-9-SAR-97Imported.
    2. Cited for: NUCLEOTIDE SEQUENCE.
      Strain: MY104-9-SAR-97Imported.
    3. Cited for: X-RAY CRYSTALLOGRAPHY (3.80 ANGSTROMS) OF 566-862; 70-323; 324-565 AND 1-69 IN COMPLEX WITH CALCIUM.
    4. "Structure of human enterovirus 71 in complex with a capsid-binding inhibitor."
      Plevka P., Perera R., Yap M.L., Cardosa J., Kuhn R.J., Rossmann M.G.
      Proc. Natl. Acad. Sci. U.S.A. 110:5463-5467(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF 566-863; 70-323; 324-565 AND 1-69.
    5. "Neutralizing antibodies can initiate genome release from human enterovirus 71."
      Plevka P., Lim P.Y., Perera R., Cardosa J., Suksatu A., Kuhn R.J., Rossmann M.G.
      Proc. Natl. Acad. Sci. U.S.A. 111:2134-2139(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY ELECTRON MICROSCOPY (10.00 ANGSTROMS) OF 566-863; 70-323 AND 324-565.

    Entry informationi

    Entry nameiA9X4C2_9ENTO
    AccessioniPrimary (citable) accession number: A9X4C2
    Entry historyi
    Integrated into UniProtKB/TrEMBL: February 5, 2008
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3