A9WYC6 (GLPK_BRUSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	BSUIS_B0444

Organism

Brucella suis (strain ATCC 23445 / NCTC 10510) [Complete proteome] [HAMAP]

Taxonomic identifier

470137 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism By similarity. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Glycerol kinase HAMAP MF_00186		PRO_1000077411
Regions
	Nucleotide binding	411 – 415	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	265	1	ATP By similarity
	Binding site	308	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9WYC6-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 82583AF6F93A0A99	FASTA	498	54,736
	10 20 30 40 50 60 MSGYILAIDQ GTTSTRSMLF DRNMRVVGLG QQEFTQHFPS SGWVEHDAEE IWKSVQSTIR 70 80 90 100 110 120 IALAQAGISA ADVAAIGITN QRKTTVVWDR ISGKPVHRAI VWQDRRTAQF CDELKRRNLE 130 140 150 160 170 180 PLFTEKTGLL LDPYFSGTKL AWLLNHVPGL RERAQKGQVC FGTIDSWLIY KLTGGKAHVT 190 200 210 220 230 240 DATNASRTLI YHIGENRWDD ELLDILGIPA AMLPEVKDCA ADFGMTDPAL FGVSIPILGV 250 260 270 280 290 300 AGDQQAAVIG NACFEPGMMK STYGTGCFAL LNTGTDRVTS SNRLLTTIAY RLDGVTTYAL 310 320 330 340 350 360 EGSIFIAGAA VQWLRDEMGF ISVASEVSAL AEKADPNQRI YLVPAFTGLG APYWDAEARG 370 380 390 400 410 420 AIFGLTRGTG RAEFARAALE SVAYQTFDLL EAMQGDWKGA TNHTVLRVDG GMVASDWTMQ 430 440 450 460 470 480 RLADILNAPV DRPVFLETTV LGAAWLAASR AGIWPDRKGF SERWQRDCRF EPAMPEKERE 490 SAIAGWRDSV SRCLTRPQ

References

[1]

"Brucella suis ATCC 23445 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000912 Genomic DNA. Translation: ABY39442.1.
RefSeq	YP_001622264.1.
3D structure databases
ProteinModelPortal	A9WYC6.
SMR	A9WYC6. Positions 4-494.
ModBase	Search...
Genome annotation databases
GeneID	5836346.
GenomeReviews	Gene locus BSUIS_B0444 in contig CP000912_GR.
KEGG	bmt:BSUIS_B0444.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG511469.
OMA	GWVEHEP.
ProtClustDB	CLSK863056.
Enzyme and pathway databases
BioCyc	BSUI470137:BSUIS_B0444-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_BRUSI

Accession

Primary (citable) accession number: A9WYC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	August 10, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)