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Protein

N-acetylneuraminate epimerase

Gene

nanM

Organism
Brucella suis (strain ATCC 23445 / NCTC 10510)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Converts alpha-N-acetylneuranimic acid (Neu5Ac) to the beta-anomer, accelerating the equilibrium between the alpha- and beta-anomers. Probably facilitates sialidase-negative bacteria to compete sucessfully for limited amounts of extracellular Neu5Ac, which is likely taken up in the beta-anomer. In addition, the rapid removal of sialic acid from solution might be advantageous to the bacterium to damp down host responses.UniRule annotation

Catalytic activityi

N-acetyl-alpha-neuraminate = N-acetyl-beta-neuraminate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciBSUI470137:GJIC-2425-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylneuraminate epimeraseUniRule annotation (EC:5.1.3.24UniRule annotation)
Alternative name(s):
N-acetylneuraminate mutarotaseUniRule annotation
Short name:
Neu5Ac mutarotaseUniRule annotation
Sialic acid epimeraseUniRule annotation
Gene namesi
Name:nanMUniRule annotation
Ordered Locus Names:BSUIS_B0415
OrganismiBrucella suis (strain ATCC 23445 / NCTC 10510)
Taxonomic identifieri470137 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000008545 Componenti: Chromosome II

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626UniRule annotationAdd
BLAST
Chaini27 – 388362N-acetylneuraminate epimerasePRO_0000333052Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati48 – 9245Kelch 1Add
BLAST
Repeati94 – 14754Kelch 2Add
BLAST
Repeati149 – 18638Kelch 3Add
BLAST
Repeati187 – 23246Kelch 4Add
BLAST
Repeati236 – 28550Kelch 5Add
BLAST
Repeati307 – 35650Kelch 6Add
BLAST
Repeati358 – 38730Kelch 7Add
BLAST

Sequence similaritiesi

Belongs to the NanM family.UniRule annotation
Contains 7 Kelch repeats.UniRule annotation

Keywords - Domaini

Kelch repeat, Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000218207.
KOiK17948.
OMAiGAAFTIQ.
OrthoDBiEOG6RC3QP.

Family and domain databases

Gene3Di2.120.10.80. 2 hits.
HAMAPiMF_01195. NanM.
InterProiIPR015915. Kelch-typ_b-propeller.
IPR019936. Mutatrotase_YjhT-like.
[Graphical view]
TIGRFAMsiTIGR03547. muta_rot_YjhT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A9WYA0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSLIGAKRQ AIGIAALAWS TGAVMASEHW PDLPVGIKNG AAARIGNMAY
60 70 80 90 100
VGLGSAGTDF YALDLNNPSK GWVKRANFIG PATNGAAMAA AGGKIFAFSG
110 120 130 140 150
NGKATPDAKS PIIFDTAYVY DPGSDGWSKL DTQTPVGLSG AKAVGLADGR
160 170 180 190 200
IAIFGGYNKE LFDKYLADVG AIDKDKEPEA YRKLVDSYMG MKPEAYRWND
210 220 230 240 250
EVLVYDPAGN NWGSLGANPF LPNCDPAMAT MGEGDFLLVS GGIKPGLRTP
260 270 280 290 300
EAKLVKIRDG AAHWQKVSDL PPLSSSEPQE GVAGAYAGKA GDDVLVAGGA
310 320 330 340 350
NFKGAQANAA AGKWFAHDGL AKSWRDDVYA FDGKDWKVAG KLPRGLAYGA
360 370 380
AFDAPGGLLV VGGEDRDGKA RKEVFLLKWD GKALSVEN
Length:388
Mass (Da):40,600
Last modified:February 5, 2008 - v1
Checksum:i96C1C1383FE02CE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000912 Genomic DNA. Translation: ABY39416.1.
RefSeqiWP_006073529.1. NC_010167.1.

Genome annotation databases

EnsemblBacteriaiABY39416; ABY39416; BSUIS_B0415.
KEGGibmt:BSUIS_B0415.
PATRICi17865226. VBIBruSui83806_0431.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000912 Genomic DNA. Translation: ABY39416.1.
RefSeqiWP_006073529.1. NC_010167.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY39416; ABY39416; BSUIS_B0415.
KEGGibmt:BSUIS_B0415.
PATRICi17865226. VBIBruSui83806_0431.

Phylogenomic databases

HOGENOMiHOG000218207.
KOiK17948.
OMAiGAAFTIQ.
OrthoDBiEOG6RC3QP.

Enzyme and pathway databases

BioCyciBSUI470137:GJIC-2425-MONOMER.

Family and domain databases

Gene3Di2.120.10.80. 2 hits.
HAMAPiMF_01195. NanM.
InterProiIPR015915. Kelch-typ_b-propeller.
IPR019936. Mutatrotase_YjhT-like.
[Graphical view]
TIGRFAMsiTIGR03547. muta_rot_YjhT. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23445 / NCTC 10510.

Entry informationi

Entry nameiNANM_BRUSI
AccessioniPrimary (citable) accession number: A9WYA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: November 11, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Lacks the conserved Glu residue in position 242, which is expected to be an active site residue.Curated

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.