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A9WWT1 (SYL_BRUSI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BSUIS_B1284
OrganismBrucella suis (strain ATCC 23445 / NCTC 10510) [Complete proteome] [HAMAP]
Taxonomic identifier470137 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length877 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 877877Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000074826

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif628 – 6325"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6311ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A9WWT1 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: 56D2AAA6E29FFD95

FASTA87797,863
        10         20         30         40         50         60 
MAAERYNPRV AEAHWQKVWE ENRTFETDNS DSREKYYVLE MFPYPSGRIH MGHVRNYAMG 

        70         80         90        100        110        120 
DVVARYKRAK GFNVLHPMGW DAFGMPAENA AMQNKVHPKE WTYQNIATMK RQLKSMGLSL 

       130        140        150        160        170        180 
DWSREFATCD VEYYHRQQML FIDLYEKGLV TRKTSKVNWD PVDNTVLANE QVVDGRGWRS 

       190        200        210        220        230        240 
GALVEQRELT QWFFKITDFS EELLAGLDTL DQWPEKVRLM QRNWIGKSEG LQVRFALAAG 

       250        260        270        280        290        300 
TAPAGFSEVE VYTTRPDTLF GAAFVAISAD HPLAKKLSEG NAALSSFIEE CHQQGTSLAA 

       310        320        330        340        350        360 
LETAEKKGFD TGIKVKHPFD DNWELPVYVA NFVLMEYGTG AVFGCPAHDQ RDLDFANKYK 

       370        380        390        400        410        420 
LKVTPVVLPK GEDAASFSIG ETAYTDDGVM INSRFLDGMT PEAAFNEVAS RLEKTDLVGR 

       430        440        450        460        470        480 
PQAVRKVQFR LRDWGISRQR YWGCPIPMIH CESCGVNPVP RADLPVKLPD DVEFDRPGNP 

       490        500        510        520        530        540 
LDRHATWRHV KCPKCGGDAR RETDTMDTFV DSSWYYTRFT APWENEPTDR KAADHWLPVD 

       550        560        570        580        590        600 
QYIGGIEHAI LHLLYSRFFT RAMKVAGHVG VDEPFKGLFT QGMVVHETYK ANGQWVSPAD 

       610        620        630        640        650        660 
IRIEEIDGKR VATMLDSGAP VEIGSIEKMS KSKKNVVDPD DIIASYGADT ARWFVLSDSP 

       670        680        690        700        710        720 
PERDVIWTEA GAEGAHRFVQ RIWRLVAEAA PALKDVAPKA GTQGEALGVS KAAHKAVKAV 

       730        740        750        760        770        780 
GDDIEKLAFN RGVARLYELV NTLSGALQQA ADGKADAEMK GALREATEML VLMTAPMMPH 

       790        800        810        820        830        840 
LAEQCLAELG GKVAGKETLV ARAPWPVFDP ALVVENEIVL PVQINGKKRG DLTIARDADQ 

       850        860        870 
ASIQQAVLEL DFVKAALNGG SPKKIIVVPQ RIVNVVA 

« Hide

References

[1]"Brucella suis ATCC 23445 whole genome shotgun sequencing project."
Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. expand/collapse author list , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S.
Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 23445 / NCTC 10510.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000912 Genomic DNA. Translation: ABY40217.1.
RefSeqYP_001623039.1. NC_010167.1.

3D structure databases

ProteinModelPortalA9WWT1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING470137.BSUIS_B1284.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY40217; ABY40217; BSUIS_B1284.
GeneID5836923.
KEGGbmt:BSUIS_B1284.
PATRIC17867091. VBIBruSui83806_1355.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycBSUI470137:GJIC-3287-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BRUSI
AccessionPrimary (citable) accession number: A9WWT1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries