A9WWR5 (HYPRE_BRUSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxyproline epimerase
EC=5.1.1.8

Alternative name(s):
Hydroxyproline-2-epimerase
Short name=HyPRE

Gene names

Ordered Locus Names:

BSUIS_B1268

Organism

Brucella suis (strain ATCC 23445 / NCTC 10510) [Complete proteome] [HAMAP]

Taxonomic identifier

470137 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows intracellular utilization of 4-hydroxyproline, one of the major constituents of host collagen, by converting 4-hydroxy-L-proline to 4-hydroxy-D-proline, which can be further metabolized by intracellular 4-hydroxy-D-proline oxidases. Strong B-cell mitogen. Plays an important role in the regulation of intra- and extracellular amino acid pools, allowing the bacterium to profit from host precursors and enzymatic pathways By similarity.
Catalytic activity	Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the proline racemase family. 4-hydroxyproline epimerase subfamily.

Ontologies

Keywords
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	4-hydroxyproline epimerase activity Inferred from electronic annotation. Source: EC proline racemase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	4-hydroxyproline epimerase		PRO_0000354031
Sites
	Active site	90	1	Proton acceptor By similarity
	Active site	253	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9WWR5 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 0963CC7E039F5717	FASTA	333	36,593
	10 20 30 40 50 60 MARHSFFCVD GHTCGNPVRL VAGGGPNLNG STMMEKRAHF LAEYDWIRTG LMFEPRGHDM 70 80 90 100 110 120 MSGSILYPPT RPDCDVAVLF IETSGCLPMC GHGTIGTVTM AIEQGLVTPK TPGKLNLDTP 130 140 150 160 170 180 AGLVAIEYEQ DGQYVERVRL TNVPAFLYAE GLEVECPDLG PIKVDVAYGG NFYAIVEPQE 190 200 210 220 230 240 NYTDMDDYSA LQLIAWSPVL RQRLNEKYKF QHPELPDINR LSHILWTGKP EHPQAHARNA 250 260 270 280 290 300 VFYGDKAIDR SPCGTGTSAR MAQLAAKGKL KPGDEFIHES IIGSLFHGRV ERAAEVAGRP 310 320 330 AIVPSIAGWA RMTGYNTIFI DDRDPFAHGF SVA

References

[1]

"Brucella suis ATCC 23445 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23445 / NCTC 10510.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000912 Genomic DNA. Translation: ABY40201.1.
RefSeq	YP_001623023.1. NC_010167.1.
3D structure databases
ProteinModelPortal	A9WWR5.
ModBase	Search...
Protein-protein interaction databases
STRING	A9WWR5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5836132.
GenomeReviews	Gene locus BSUIS_B1268 in contig CP000912_GR.
KEGG	bmt:BSUIS_B1268.
PATRIC	17867061. VBIBruSui83806_1340.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG333420.
OMA	CNIPAFL.
ProtClustDB	PRK13971.
Enzyme and pathway databases
BioCyc	BSUI470137:BSUIS_B1268-MONOMER.
Family and domain databases
InterPro	IPR008794. Pro_racemase. [Graphical view]
KO	K12658.
PANTHER	PTHR18835. PTHR18835. 1 hit.
Pfam	PF05544. Pro_racemase. 1 hit. [Graphical view]
PIRSF	PIRSF029792. Pro_racemase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HYPRE_BRUSI

Accession

Primary (citable) accession number: A9WWR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program