A9WUC0 (A9WUC0_RENSM) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193
EC=6.3.1.5 HAMAP MF_00193

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	RSal33209_3070

Organism

Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) [Complete proteome] [HAMAP] EMBL ABY24791.1

Taxonomic identifier

288705 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Renibacterium

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 SAAS SAAS003694
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 SAAS SAAS003694
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 SAAS SAAS003694 NAD HAMAP MF_00193 SAAS SAAS003694 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 SAAS SAAS003694 EMBL ABY24791.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	48	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	A9WUC0 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 98474CAA524BE4F7	FASTA	273	29,783
	10 20 30 40 50 60 MRELQAKIIA ELGVKPTIDP AAEVTKRVDF LVDYAKATRT KGFVLGISGG LDSSLAGKLG 70 80 90 100 110 120 QLAAEKLRAE GLDASFTAVR LPYNVQHDEA DAQAALAFIQ PDRSWTCNVA PGVDGLEKEY 130 140 150 160 170 180 KATTGEQLSD FNRGNIKARA RMVAQYALAG EHGLLVIGTD HGAESVTGFF TKFGDGGADI 190 200 210 220 230 240 LPLFTLNKRQ NRQLLTYLGA AERIYQKVPT ADLLDGVPGR ADEDELGLSY DDIDDYLEGR 250 260 270 EVSDAVAEAI EKRFLATRHK RTVPVTLLDD WWK

References

[1]

"Genome sequence of the fish pathogen Renibacterium salmoninarum suggests reductive evolution away from an environmental Arthrobacter ancestor." Wiens G.D., Rockey D.D., Wu Z., Chang J., Levy R., Crane S., Chen D.S., Capri G.R., Burnett J.R., Sudheesh P.S., Schipma M.J., Burd H., Bhattacharyya A., Rhodes L.D., Kaul R., Strom M.S. J. Bacteriol. 190:6970-6982(2008) [PubMed: 18723615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000910 Genomic DNA. Translation: ABY24791.1.
RefSeq	YP_001626205.1. NC_010168.1.
3D structure databases
ProteinModelPortal	A9WUC0.
SMR	A9WUC0. Positions 4-273.
ModBase	Search...
Protein-protein interaction databases
STRING	A9WUC0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5821681.
GenomeReviews	Gene locus RSal33209_3070 in contig CP000910_GR.
KEGG	rsa:RSal33209_3070.
PATRIC	23079591. VBIRenSal21953_3200.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	IAQYEIA.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	RSAL288705:RSAL33209_3070-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A9WUC0_RENSM

Accession

Primary (citable) accession number: A9WUC0

Entry history

Integrated into UniProtKB/TrEMBL:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)