A9WTD8 (A9WTD8_RENSM) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL ABY24459.1
EC=5.99.1.2 EMBL ABY24459.1

Gene names

Ordered Locus Names:

RSal33209_2734

Organism

Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) [Complete proteome] [HAMAP] EMBL ABY24459.1

Taxonomic identifier

288705 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Renibacterium

Protein attributes

Sequence length	912 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A9WTD8 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: C7530ABD7A3977D5	FASTA	912	99,501
	10 20 30 40 50 60 MPTKASSKRA LTGKKLVIVE SPAKGKTISG YLGEGFEVEA SYGHIRDLPQ PSDLPAELKK 70 80 90 100 110 120 SSVGKFAVDL DNNFEPYYVV PGDKKKRVAE LKAALKDADE LYLATDGDRE GEAIAWHLLE 130 140 150 160 170 180 VLKPKVPVYR LTFPEITKEA IQRAMGELRD VDMPMVDAQE TRRVLDRLYG YEISPVLWRK 190 200 210 220 230 240 VSRGLSAGRV QSVATRLVVE RERERMAFRS ASYWDLSGEF TPENSAQAFK AKLVALDGAR 250 260 270 280 290 300 VATGRDFNDT GVLTSKNVAH LDEAIAAALA AGLQQAKFAV RSVENKPYTR RPAAPFTTST 310 320 330 340 350 360 LQQEAARKLW FSARVTMRVA QSLYENGYIT YMRTDSTALS SQAVNAARRQ AAELYDPSFV 370 380 390 400 410 420 PDAPRVYAGK AKNAQEAHEA IRPAGDSFRI PAQVAKALSS DEFKLYELIW KRTVASQMAD 430 440 450 460 470 480 AKGSTASVRL GAVATGGQNA EFSASGTVIT FQGFLAAYEE GRDEGRNKDD ADSDGGRLPN 490 500 510 520 530 540 LAAQDALTAT EIEPLGHETS PPPRYTEASL VKTLEERGIG RPSTYAATIS TIMDRGYVSQ 550 560 570 580 590 600 RGSALVPSWV AFSVVRLLEQ YFQNYVDYDF TAGMEEDLDR ISRGEETGPH WLKLFYFGDE 610 620 630 640 650 660 DGSGLLPIVN NLGEIDAREI NSVPISDGIT LRVGKFGPYL EKPVPADAPE GTEPERANVP 670 680 690 700 710 720 EDLAPDELTA EKAIELINTA APEERVLGVD PISGHSVVAK NGRYGAYVTE LIPEPTAEDL 730 740 750 760 770 780 ANAPVEYYKN GKPKPAKKPV KAKPRTGSLF ASMSVENVSL DEALQLMSLP RVLGADAEGN 790 800 810 820 830 840 EITVQNGRFG PYLKKGSDSR SIGSEEEIFT ITLEQALEIY SQPKQRGARA AVPPLAEFGD 850 860 870 880 890 900 DPTSGKKIVV KEGRFGPYIT DGETNITVPR STSIEELTRE RAVELLADKR AKGPVKRPAK 910 RPAAKAKAGS RR

References

[1]

"Genome sequence of the fish pathogen Renibacterium salmoninarum suggests reductive evolution away from an environmental Arthrobacter ancestor." Wiens G.D., Rockey D.D., Wu Z., Chang J., Levy R., Crane S., Chen D.S., Capri G.R., Burnett J.R., Sudheesh P.S., Schipma M.J., Burd H., Bhattacharyya A., Rhodes L.D., Kaul R., Strom M.S. J. Bacteriol. 190:6970-6982(2008) [PubMed: 18723615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000910 Genomic DNA. Translation: ABY24459.1.
RefSeq	YP_001625873.1. NC_010168.1.
3D structure databases
ProteinModelPortal	A9WTD8.
ModBase	Search...
Protein-protein interaction databases
STRING	A9WTD8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5824550.
GenomeReviews	Gene locus RSal33209_2734 in contig CP000910_GR.
KEGG	rsa:RSal33209_2734.
PATRIC	23078883. VBIRenSal21953_2850.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	KKVMPRL.
ProtClustDB	CLSK960232.
Enzyme and pathway databases
BioCyc	RSAL288705:RSAL33209_2734-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A9WTD8_RENSM

Accession

Primary (citable) accession number: A9WTD8

Entry history

Integrated into UniProtKB/TrEMBL:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)