A9WS93 (GLPK_RENSM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	RSal33209_1948

Organism

Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) [Complete proteome] [HAMAP]

Taxonomic identifier

288705 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Renibacterium

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism By similarity. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Glycerol kinase HAMAP MF_00186		PRO_1000077425
Regions
	Nucleotide binding	413 – 417	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	246	1	Substrate By similarity
	Binding site	268	1	ATP By similarity
	Binding site	312	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9WS93 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 52668EB8A086E3CD	FASTA	504	54,633
	10 20 30 40 50 60 MSQYVIAIDQ GTTSSRAIIF DHDGNIVSTG QLEHEQIFPK AGWVEHNATE IWNNTREVIG 70 80 90 100 110 120 TALSKANLTR HDIAAVGITN QRETAVVWDK TTGEPVYNAI VWQDTRTQSI VDELAVGGGV 130 140 150 160 170 180 ERFKDKVGLP LATYFSGTKI KWILDNVDGA RERAAAGDLL FGNTDSWVTW NLTGGVDGGV 190 200 210 220 230 240 HITDVTNASR TMFMDLATLS WDQEILDAFG VPASMLPTIK SSSEVYGTVH TSQLLREVPV 250 260 270 280 290 300 AGILGDQQAA TFGQAAFNTG EAKNTYGTGC FLIFNTGEEI VHSKNGLLTT VGYKLGDAPT 310 320 330 340 350 360 HYALEGSIAV AGSLIQWLRD TLGMISSAPE VEELAASVED NGGVYIVPAF SGLFAPYWRS 370 380 390 400 410 420 DARGAIVGMT RYVNRNHIAR AALEATAFQT REVLDAVNAD SGVPLTELKV DGGMVANDAL 430 440 450 460 470 480 MQFQADILGV PVIRPKVTET TALGAAYAAG LAVGFWNDLG ELSSNWAEDK RWEPAMEEDE 490 500 RARQLRLWKK AVTKSMDWVD EDVR

References

[1]

"Genome sequence of the fish pathogen Renibacterium salmoninarum suggests reductive evolution away from an environmental Arthrobacter ancestor." Wiens G.D., Rockey D.D., Wu Z., Chang J., Levy R., Crane S., Chen D.S., Capri G.R., Burnett J.R., Sudheesh P.S., Schipma M.J., Burd H., Bhattacharyya A., Rhodes L.D., Kaul R., Strom M.S. J. Bacteriol. 190:6970-6982(2008) [PubMed: 18723615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000910 Genomic DNA. Translation: ABY23681.1.
RefSeq	YP_001625095.1. NC_010168.1.
3D structure databases
ProteinModelPortal	A9WS93.
SMR	A9WS93. Positions 2-503.
ModBase	Search...
Protein-protein interaction databases
STRING	A9WS93.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5822656.
GenomeReviews	Gene locus RSal33209_1948 in contig CP000910_GR.
KEGG	rsa:RSal33209_1948.
PATRIC	23077235. VBIRenSal21953_2034.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG511469.
OMA	ATYFSGT.
ProtClustDB	CLSK928226.
Enzyme and pathway databases
BioCyc	RSAL288705:RSAL33209_1948-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_RENSM

Accession

Primary (citable) accession number: A9WS93

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program