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A9WS85 (SYL_RENSM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:RSal33209_1940
OrganismRenibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) [Complete proteome] [HAMAP]
Taxonomic identifier288705 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeRenibacterium

Protein attributes

Sequence length862 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence ABY23673.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 862862Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334801

Regions

Motif79 – 8911"HIGH" region HAMAP-Rule MF_00049
Motif627 – 6315"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6301ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A9WS85 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: F244F35CF99EC356

FASTA86295,215
        10         20         30         40         50         60 
MVQSRGFSLS SVRNGTEGNT MSQHTSQPAA DVPVDVADEN RYDIAAIEAK WLPRWEEANI 

        70         80         90        100        110        120 
FAPLDDGSKE RRYVCDMFPY PSGDLHMGHA EAFVMGDVVG RYWKLKGFDV LHPVGWDSFG 

       130        140        150        160        170        180 
LPAENAAIKR NAHPAEWTYA NIETQAASFK RYAIGVDWSR RLQTSDPEYY RWTQWLFLRF 

       190        200        210        220        230        240 
YERGLAYRKN SPVNWCPKDL TVLANEQVIN GACERCGTPV TKKNLNQWYF KITDYADRLL 

       250        260        270        280        290        300 
DDMAQLEGHW PERVLAMQRN WIGRSQGAHV DFVIDGMSDA ETGEAKKVTV FTTRPDTLYG 

       310        320        330        340        350        360 
ATFFAVAVDA ALADELVTEE QRAELSAYQE RVKHLSDIER QESDREKTGV FLGRYAINPI 

       370        380        390        400        410        420 
DGEMLPIWAA DYVLADYGTG AIMAVPAHDQ RDLDFARAFD LPVRQVLDTG AENPAETGTA 

       430        440        450        460        470        480 
AIGDGVLVNS GELDGLAKAE AIPAAISLLS KIGTGEATTN YRLRDWLLSR QRFWGAPVPI 

       490        500        510        520        530        540 
IHCAECGEVP VPDDQLPVRL PENLRGEQLA PKGTSPLASV ADWVNVDCPK CGGAATRDTD 

       550        560        570        580        590        600 
TIDTFVDSSW YYLRYTSPEY TDGPFDPAAA TRWMGVDQYV GGVEHAILHL LYSRFFVKVL 

       610        620        630        640        650        660 
NDLGLLEAKE PFKALLNQGQ VLNGGKAMSK SLGNGVDLSQ QLDRFGVDAV RLTMVFASPP 

       670        680        690        700        710        720 
EDDVDWADVS PSGSAKFLAR AWRLAQDVTS EIGVDPATGD RALRSVTHRT LSEAADLIDA 

       730        740        750        760        770        780 
HKFNVVVAKT MELVNITRKS IDSGCGPADP AVREAVEAVA VILSLFAPYT AEDIWAELGH 

       790        800        810        820        830        840 
QDFVARASWP TVDDSLLVQS TITAVVQVQG KVRDRLEVPT DITEDQLREL ALASEIVQKT 

       850        860 
LDGRGIRTVI VRAPKLVNIV PA 

« Hide

References

[1]"Genome sequence of the fish pathogen Renibacterium salmoninarum suggests reductive evolution away from an environmental Arthrobacter ancestor."
Wiens G.D., Rockey D.D., Wu Z., Chang J., Levy R., Crane S., Chen D.S., Capri G.R., Burnett J.R., Sudheesh P.S., Schipma M.J., Burd H., Bhattacharyya A., Rhodes L.D., Kaul R., Strom M.S.
J. Bacteriol. 190:6970-6982(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000910 Genomic DNA. Translation: ABY23673.1. Different initiation.
RefSeqYP_001625087.1. NC_010168.1.

3D structure databases

ProteinModelPortalA9WS85.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING288705.RSal33209_1940.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY23673; ABY23673; RSal33209_1940.
GeneID5822648.
KEGGrsa:RSal33209_1940.
PATRIC23077217. VBIRenSal21953_2025.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycRSAL288705:GHX1-1940-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RENSM
AccessionPrimary (citable) accession number: A9WS85
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries