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A9WP47 (TRMD_RENSM) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase

EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene names
Name:trmD
Ordered Locus Names:RSal33209_1084
OrganismRenibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) [Complete proteome] [HAMAP]
Taxonomic identifier288705 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeRenibacterium

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP-Rule MF_00605

Catalytic activity

S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine37 in tRNA. HAMAP-Rule MF_00605

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00605

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00605.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine(37)-N(1))-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 263263tRNA (guanine-N(1)-)-methyltransferase HAMAP-Rule MF_00605
PRO_1000082530

Regions

Region137 – 1426S-adenosyl-L-methionine binding By similarity

Sites

Binding site1131S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
A9WP47 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: 49994713A46215AC

FASTA26328,850
        10         20         30         40         50         60 
MRLDVISIFP DYLAPLELSL IGKARQDGLL ELNVHDLRAF TTDRHRTVDD TPYGGGAGMV 

        70         80         90        100        110        120 
MKPEPWALAL ASVVEPAAKS KPVLIVPSPA GEVFNQRIAE ELAAESHLVF ACGRYEGIDE 

       130        140        150        160        170        180 
RVVDWARESF TVRPMSLGDY VLNGGEVAVM AMVEATARLL PGVVGNPDSL LEESHQDGLL 

       190        200        210        220        230        240 
EYPVYTKPSA WRGVDIPAVL LSGNHAKIAR FRRDEQLRRT AQRRPDLLTQ LDPAILDRAD 

       250        260 
LAVLRESGYL TVDGVLTRTS ELG 

« Hide

References

[1]"Genome sequence of the fish pathogen Renibacterium salmoninarum suggests reductive evolution away from an environmental Arthrobacter ancestor."
Wiens G.D., Rockey D.D., Wu Z., Chang J., Levy R., Crane S., Chen D.S., Capri G.R., Burnett J.R., Sudheesh P.S., Schipma M.J., Burd H., Bhattacharyya A., Rhodes L.D., Kaul R., Strom M.S.
J. Bacteriol. 190:6970-6982(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000910 Genomic DNA. Translation: ABY22822.1.
RefSeqYP_001624236.1. NC_010168.1.

3D structure databases

ProteinModelPortalA9WP47.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING288705.RSal33209_1084.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY22822; ABY22822; RSal33209_1084.
GeneID5822172.
KEGGrsa:RSal33209_1084.
PATRIC23075387. VBIRenSal21953_1114.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0336.
HOGENOMHOG000016242.
KOK00554.
OMAWRGHEVP.
OrthoDBEOG6J48RZ.

Enzyme and pathway databases

BioCycRSAL288705:GHX1-1084-MONOMER.

Family and domain databases

Gene3D1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPMF_00605. TrmD.
InterProIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMSSF75217. SSF75217. 1 hit.
TIGRFAMsTIGR00088. trmD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMD_RENSM
AccessionPrimary (citable) accession number: A9WP47
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: June 11, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families