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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.UniRule annotationSAAS annotation

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotationSAAS annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. methionine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. methionyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciRSAL288705:GHX1-1051-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligaseUniRule annotationSAAS annotation (EC:6.1.1.10UniRule annotationSAAS annotation)
Alternative name(s):
Methionyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:metGUniRule annotation
Ordered Locus Names:RSal33209_1051Imported
OrganismiRenibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235)Imported
Taxonomic identifieri288705 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeRenibacterium
ProteomesiUP000002007 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi288705.RSal33209_1051.

Structurei

3D structure databases

ProteinModelPortaliA9WP14.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi305 – 3095"KMSKS" regionUniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0143.
HOGENOMiHOG000200401.
KOiK01874.
OMAiGVFPRYV.
OrthoDBiEOG6CVV9B.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9WP14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTESSNKRPY YLTTAITYPN GVPHIGHAYE YVSADALARF KRLDGFDVFY
60 70 80 90 100
MSGTDEHGIK VQQAAEKEGV TPLELVDRNS AAIRAVHDLV ESSYDRFIRT
110 120 130 140 150
TEPEHYLASQ ELWRRMEANG DIYLDKYTGW YSVRDEAYYT DEQTELRADG
160 170 180 190 200
IRYAIETETE VTWTEEESYF FRLSNYQEKL LALYEAQPEF AAPQYRFNEV
210 220 230 240 250
ISFVKSGLQD LSISRTSFNW GVPVPGNDKH VMYVWVDALT NYLTGAGFPD
260 270 280 290 300
ESSSQWKYWP ADVHIIGKDI SRFHAIFWPG FLMSAGLPLP KRVMIHGFLF
310 320 330 340 350
NNGVKMSKSF GNVVAPADWV AAYGVDAVRF FFLREVSYGQ DGNYSHEAII
360 370 380 390 400
SRKNSDLANN LGNLAQRSLS MVAKNCDGVV PTPGEFSSAD QAILAKAQAL
410 420 430 440 450
LAANRASYEV QEFHRALEAI WAVLGDTNAY FADQAPWVLR KTDPERMATV
460 470 480 490 500
LYVTLEVLRI VALLIQPVMP GSAAKLLDVL GVSAAEGARD FTAVETPLVA
510 520
GTALPAPSPI FPRYEEPAEA SAHGKPQTH
Length:529
Mass (Da):59,191
Last modified:February 5, 2008 - v1
Checksum:i30D9647955EA8BA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000910 Genomic DNA. Translation: ABY22789.1.
RefSeqiWP_012244480.1. NC_010168.1.
YP_001624203.1. NC_010168.1.

Genome annotation databases

EnsemblBacteriaiABY22789; ABY22789; RSal33209_1051.
KEGGirsa:RSal33209_1051.
PATRICi23075311. VBIRenSal21953_1076.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000910 Genomic DNA. Translation: ABY22789.1.
RefSeqiWP_012244480.1. NC_010168.1.
YP_001624203.1. NC_010168.1.

3D structure databases

ProteinModelPortaliA9WP14.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi288705.RSal33209_1051.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY22789; ABY22789; RSal33209_1051.
KEGGirsa:RSal33209_1051.
PATRICi23075311. VBIRenSal21953_1076.

Phylogenomic databases

eggNOGiCOG0143.
HOGENOMiHOG000200401.
KOiK01874.
OMAiGVFPRYV.
OrthoDBiEOG6CVV9B.

Enzyme and pathway databases

BioCyciRSAL288705:GHX1-1051-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the fish pathogen Renibacterium salmoninarum suggests reductive evolution away from an environmental Arthrobacter ancestor."
    Wiens G.D., Rockey D.D., Wu Z., Chang J., Levy R., Crane S., Chen D.S., Capri G.R., Burnett J.R., Sudheesh P.S., Schipma M.J., Burd H., Bhattacharyya A., Rhodes L.D., Kaul R., Strom M.S.
    J. Bacteriol. 190:6970-6982(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235Imported.

Entry informationi

Entry nameiA9WP14_RENSM
AccessioniPrimary (citable) accession number: A9WP14
Entry historyi
Integrated into UniProtKB/TrEMBL: February 5, 2008
Last sequence update: February 5, 2008
Last modified: April 1, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.