A9WMW3 (FTHS_RENSM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	RSal33209_1710

Organism

Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) [Complete proteome] [HAMAP]

Taxonomic identifier

288705 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Renibacterium

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_0000333316
Regions
	Nucleotide binding	69 – 76	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9WMW3 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: DC6559FB43FE7A3D	FASTA	564	59,860
	10 20 30 40 50 60 MTSTTEPMTD LHIAQQAVLH PIFDIADAAG IPEEALEQYG RYKAKVDVRK VPDSGRAGRV 70 80 90 100 110 120 VLVTAVSPTP AGEGKSTTTV GLADSLNRAF EQEGTGRRSM IALREPSLGP TLGMKGGATG 130 140 150 160 170 180 GGYSQVLPMD EINLHFTGDL HAINSANNAL CALIDNHIYQ GNVLNIDPRR ITFKRVLDMN 190 200 210 220 230 240 DRALREVVIG LGGPTQGVPR QDGFDITVAS EIMAVFCLAT DLNDLKSRIG KITFGYNYDR 250 260 270 280 290 300 QPLTVAGLGV EGVLTLLLKE AIKPNLVQTL AGTAALVHGG PFANIAHGCN SVIATSLARR 310 320 330 340 350 360 RADVVVTEAG FGADLGAEKY MDIKSRFADV APSAVVIVAT IRALKMHGGV PKTELSVSDV 370 380 390 400 410 420 AALRRGVTNL ARHISNVRQF GLDPVVSINR FTSDSEEELD WLVSWCESQG VSIAIADVWG 430 440 450 460 470 480 RGGGGDDLAA KVLAALDAPS DFRHLYELEL PVKEKIELIA QKIYGAERVE FSSSALKRIA 490 500 510 520 530 540 EISANGWDSL PVCMAKTQYS FSDDASLLGA PSGFVLHVRD LVPKTGAGFI VALTGAVMTM 550 560 PGLPKQPAAL KMDVDAEGNS VGLS

References

[1]

"Genome sequence of the fish pathogen Renibacterium salmoninarum suggests reductive evolution away from an environmental Arthrobacter ancestor." Wiens G.D., Rockey D.D., Wu Z., Chang J., Levy R., Crane S., Chen D.S., Capri G.R., Burnett J.R., Sudheesh P.S., Schipma M.J., Burd H., Bhattacharyya A., Rhodes L.D., Kaul R., Strom M.S. J. Bacteriol. 190:6970-6982(2008) [PubMed: 18723615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000910 Genomic DNA. Translation: ABY23446.1.
RefSeq	YP_001624860.1. NC_010168.1.
3D structure databases
ProteinModelPortal	A9WMW3.
SMR	A9WMW3. Positions 10-562.
ModBase	Search...
Protein-protein interaction databases
STRING	A9WMW3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5822435.
GenomeReviews	Gene locus RSal33209_1710 in contig CP000910_GR.
KEGG	rsa:RSal33209_1710.
PATRIC	23076715. VBIRenSal21953_1777.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG677721.
OMA	EIMAVLC.
ProtClustDB	PRK13505.
Enzyme and pathway databases
BioCyc	RSAL288705:RSAL33209_1710-MONOMER.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
KO	K01938.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_RENSM

Accession

Primary (citable) accession number: A9WMW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program