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A9WL73

- PCKG_RENSM

UniProt

A9WL73 - PCKG_RENSM

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei81 – 811SubstrateUniRule annotation
    Binding sitei223 – 2231Substrate; via amide nitrogenUniRule annotation
    Metal bindingi230 – 2301ManganeseUniRule annotation
    Binding sitei230 – 2301SubstrateUniRule annotation
    Metal bindingi250 – 2501Manganese; via tele nitrogenUniRule annotation
    Binding sitei272 – 2721SubstrateUniRule annotation
    Active sitei274 – 2741UniRule annotation
    Metal bindingi297 – 2971ManganeseUniRule annotation
    Binding sitei390 – 3901GTPUniRule annotation
    Binding sitei421 – 4211GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi273 – 2786GTPUniRule annotation
    Nucleotide bindingi516 – 5194GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciRSAL288705:GHX1-29-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:RSal33209_0029
    OrganismiRenibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235)
    Taxonomic identifieri288705 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeRenibacterium
    ProteomesiUP000002007: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 607607Phosphoenolpyruvate carboxykinase [GTP]PRO_1000080987Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi288705.RSal33209_0029.

    Structurei

    3D structure databases

    ProteinModelPortaliA9WL73.
    SMRiA9WL73. Positions 12-607.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni388 – 3903Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiGNNIFTN.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A9WL73-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLTAQPVLD TAPTTNKRLL DWVAEVAELT QPESIYWVDG SEAENTRLTD    50
    ELVAAGTLTR LNPELFPNSF AGFSDPKDVA RVEEQTFICS EKEHDAGFTN 100
    NWMEPAEMRT KLSGLFKGSM RGRTMYVIPF VMGHLDAKSP KFGVEITDSA 150
    YVVASMRIMA RIGTEVLRKI EEPDAFFVPA IHSLGAPLEP GQADVPWPCN 200
    DEKWIVHFPE SREIWSYGSG YGGNALLGKK CYALRIASVM AHDEGWLAEH 250
    MLILKLTSPE QKTYYMAAAF PSACGKTNLA LLDPTIKGWK VETLGDDITW 300
    MNFDDEGALR AVNPEAGLFG VAPGTGWDTN PNAMRAIAKG NSIFTNVALT 350
    DDGGVWWEGM TTETPSHLTD WRGNSWTPDS AEPAAHPNSR FCTPIDQIDM 400
    LAPEYNEPDG VPLSAILFGG RRKTTIPLVT QSRDWTNGIF MGSTLSSETT 450
    AAAAGAVGVV RRDPMAMLPF IGYDAGDYLK HWVELSKQGD QEKLPKIFLV 500
    NWFRRTADGG FAWPGFGDNS RVVTWAIERI EGKADAVETP IGFVPTKESL 550
    DLDGLDISDE QLSAALNVDA LEWAAEAESI DEWYRRFGGS LPEELLGELE 600
    GLKERLG 607
    Length:607
    Mass (Da):66,756
    Last modified:February 5, 2008 - v1
    Checksum:i12E563E9DAE4DBA0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000910 Genomic DNA. Translation: ABY21787.1.
    RefSeqiYP_001623201.1. NC_010168.1.

    Genome annotation databases

    EnsemblBacteriaiABY21787; ABY21787; RSal33209_0029.
    GeneIDi5822592.
    KEGGirsa:RSal33209_0029.
    PATRICi23073176. VBIRenSal21953_0029.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000910 Genomic DNA. Translation: ABY21787.1 .
    RefSeqi YP_001623201.1. NC_010168.1.

    3D structure databases

    ProteinModelPortali A9WL73.
    SMRi A9WL73. Positions 12-607.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 288705.RSal33209_0029.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABY21787 ; ABY21787 ; RSal33209_0029 .
    GeneIDi 5822592.
    KEGGi rsa:RSal33209_0029.
    PATRICi 23073176. VBIRenSal21953_0029.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi GNNIFTN.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci RSAL288705:GHX1-29-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the fish pathogen Renibacterium salmoninarum suggests reductive evolution away from an environmental Arthrobacter ancestor."
      Wiens G.D., Rockey D.D., Wu Z., Chang J., Levy R., Crane S., Chen D.S., Capri G.R., Burnett J.R., Sudheesh P.S., Schipma M.J., Burd H., Bhattacharyya A., Rhodes L.D., Kaul R., Strom M.S.
      J. Bacteriol. 190:6970-6982(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235.

    Entry informationi

    Entry nameiPCKG_RENSM
    AccessioniPrimary (citable) accession number: A9WL73
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3