Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A9WL73 (PCKG_RENSM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]

Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Gene names
Name:pckG
Ordered Locus Names:RSal33209_0029
OrganismRenibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) [Complete proteome] [HAMAP]
Taxonomic identifier288705 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeRenibacterium

Protein attributes

Sequence length607 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00452

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 607607Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452
PRO_1000080987

Regions

Nucleotide binding273 – 2786GTP By similarity
Nucleotide binding516 – 5194GTP By similarity
Region388 – 3903Substrate binding By similarity

Sites

Active site2741 By similarity
Metal binding2301Manganese By similarity
Metal binding2501Manganese; via tele nitrogen By similarity
Metal binding2971Manganese By similarity
Binding site811Substrate By similarity
Binding site2231Substrate; via amide nitrogen By similarity
Binding site2301Substrate By similarity
Binding site2721Substrate By similarity
Binding site3901GTP By similarity
Binding site4211GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
A9WL73 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: 12E563E9DAE4DBA0

FASTA60766,756
        10         20         30         40         50         60 
MGLTAQPVLD TAPTTNKRLL DWVAEVAELT QPESIYWVDG SEAENTRLTD ELVAAGTLTR 

        70         80         90        100        110        120 
LNPELFPNSF AGFSDPKDVA RVEEQTFICS EKEHDAGFTN NWMEPAEMRT KLSGLFKGSM 

       130        140        150        160        170        180 
RGRTMYVIPF VMGHLDAKSP KFGVEITDSA YVVASMRIMA RIGTEVLRKI EEPDAFFVPA 

       190        200        210        220        230        240 
IHSLGAPLEP GQADVPWPCN DEKWIVHFPE SREIWSYGSG YGGNALLGKK CYALRIASVM 

       250        260        270        280        290        300 
AHDEGWLAEH MLILKLTSPE QKTYYMAAAF PSACGKTNLA LLDPTIKGWK VETLGDDITW 

       310        320        330        340        350        360 
MNFDDEGALR AVNPEAGLFG VAPGTGWDTN PNAMRAIAKG NSIFTNVALT DDGGVWWEGM 

       370        380        390        400        410        420 
TTETPSHLTD WRGNSWTPDS AEPAAHPNSR FCTPIDQIDM LAPEYNEPDG VPLSAILFGG 

       430        440        450        460        470        480 
RRKTTIPLVT QSRDWTNGIF MGSTLSSETT AAAAGAVGVV RRDPMAMLPF IGYDAGDYLK 

       490        500        510        520        530        540 
HWVELSKQGD QEKLPKIFLV NWFRRTADGG FAWPGFGDNS RVVTWAIERI EGKADAVETP 

       550        560        570        580        590        600 
IGFVPTKESL DLDGLDISDE QLSAALNVDA LEWAAEAESI DEWYRRFGGS LPEELLGELE 


GLKERLG 

« Hide

References

[1]"Genome sequence of the fish pathogen Renibacterium salmoninarum suggests reductive evolution away from an environmental Arthrobacter ancestor."
Wiens G.D., Rockey D.D., Wu Z., Chang J., Levy R., Crane S., Chen D.S., Capri G.R., Burnett J.R., Sudheesh P.S., Schipma M.J., Burd H., Bhattacharyya A., Rhodes L.D., Kaul R., Strom M.S.
J. Bacteriol. 190:6970-6982(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000910 Genomic DNA. Translation: ABY21787.1.
RefSeqYP_001623201.1. NC_010168.1.

3D structure databases

ProteinModelPortalA9WL73.
SMRA9WL73. Positions 12-607.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING288705.RSal33209_0029.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY21787; ABY21787; RSal33209_0029.
GeneID5822592.
KEGGrsa:RSal33209_0029.
PATRIC23073176. VBIRenSal21953_0029.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHOG000191700.
KOK01596.
OMAGNNIFTN.
OrthoDBEOG6MPWQS.

Enzyme and pathway databases

BioCycRSAL288705:GHX1-29-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_RENSM
AccessionPrimary (citable) accession number: A9WL73
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways