ID   FTHS_CHLAA              Reviewed;         572 AA.
AC   A9WIW3;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   27-MAR-2024, entry version 77.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=Caur_2634;
OS   Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl).
OC   Bacteria; Chloroflexota; Chloroflexia; Chloroflexales; Chloroflexineae;
OC   Chloroflexaceae; Chloroflexus.
OX   NCBI_TaxID=324602;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29366 / DSM 635 / J-10-fl;
RX   PubMed=21714912; DOI=10.1186/1471-2164-12-334;
RA   Tang K.H., Barry K., Chertkov O., Dalin E., Han C.S., Hauser L.J.,
RA   Honchak B.M., Karbach L.E., Land M.L., Lapidus A., Larimer F.W.,
RA   Mikhailova N., Pitluck S., Pierson B.K., Blankenship R.E.;
RT   "Complete genome sequence of the filamentous anoxygenic phototrophic
RT   bacterium Chloroflexus aurantiacus.";
RL   BMC Genomics 12:334-334(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000909; ABY35840.1; -; Genomic_DNA.
DR   RefSeq; WP_012258493.1; NC_010175.1.
DR   RefSeq; YP_001636229.1; NC_010175.1.
DR   AlphaFoldDB; A9WIW3; -.
DR   SMR; A9WIW3; -.
DR   STRING; 324602.Caur_2634; -.
DR   EnsemblBacteria; ABY35840; ABY35840; Caur_2634.
DR   KEGG; cau:Caur_2634; -.
DR   PATRIC; fig|324602.8.peg.2969; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_0; -.
DR   InParanoid; A9WIW3; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000002008; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0004477; F:methenyltetrahydrofolate cyclohydrolase activity; IBA:GO_Central.
DR   GO; GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IBA:GO_Central.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IBA:GO_Central.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..572
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_1000087649"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   572 AA;  60580 MW;  25EFDFB2BF369EF9 CRC64;
     MKTSLQIAAE ARLEPIAAIA ERLGLPVRYL EPYGRYRGKI DLTFLDDYHD RPLGRYVLVS
     AITPTPLGEG KTTTAIGLAM ALNRIGKRAA VTLRQSSLGP VFGIKGGGAG GGYSQIVPLV
     ESILHLNGDI HAVSQAHNQL AALTDNSWYH GNPLDIDPDR IEIRRVVDVN DRFLRQVMIG
     LGGKQNGFPR QTGFDISVAS ELMAILAMVN GVGARAALRD LRSRIGRMVV AFRRDGTPIT
     AEDVRGAGAA TVLMREALKP NLMQTIENTP ALIHAGPFAN IAQGNSSILA DLIALRCADY
     VVTEAGFGVD IGAEKFFNLK CRASGLWPDV AVIVATIRAL KAHSGKYDIV AGKPLPPALL
     HENPDDVISG GANLRRQIEN LHQFKVPVIV ALNAYPEDTP AEIDAVAHIA TTAGAAGMAV
     SNVYAAGSAG GVDLARLVIE IAERPGPRPV QFLYPLEWSL ADKITTIAHR IYGAAAVTFS
     PTAAAQLAAL EDAGFGNLPI CMAKTHLSLS HDPALRGAPE GFTFPIREVR LSAGAGFILP
     IAGTTVTMPG LGAHPAAHQI DIDDEGNIVG LF
//