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A9WHH4 (TRMB_CHLAA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Caur_3101
OrganismChloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) [Reference proteome] [HAMAP]
Taxonomic identifier324602 [NCBI]
Taxonomic lineageBacteriaChloroflexiChloroflexalesChloroflexaceaeChloroflexus

Protein attributes

Sequence length224 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 224224tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000387942

Sites

Binding site571S-adenosyl-L-methionine By similarity
Binding site821S-adenosyl-L-methionine By similarity
Binding site1091S-adenosyl-L-methionine By similarity
Binding site1671Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A9WHH4 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: A30622F44EFDBF20

FASTA22424,960
        10         20         30         40         50         60 
MGRGRYPARL YVTAPPPEIA ARYYRSWPAK ELYHTPEHFP PVSAEGLFGY NAPLTLEFGC 

        70         80         90        100        110        120 
ATGEYLCALA AAQPSSCFVG IDIVAKPLYR AVERAVAGNL SNILFIHADA RLIYQRIPTA 

       130        140        150        160        170        180 
ALHSIILHFP PPLLRNRQRN QLLVSPQLLE CAARTLVPGG YLSFLTDHPA LFALMQELLP 

       190        200        210        220 
AFPALRATPA SVEELAVFES HYHRRWAARG RTISGLRIER VMED 

« Hide

References

[1]"Complete genome sequence of the filamentous anoxygenic phototrophic bacterium Chloroflexus aurantiacus."
Tang K.H., Barry K., Chertkov O., Dalin E., Han C.S., Hauser L.J., Honchak B.M., Karbach L.E., Land M.L., Lapidus A., Larimer F.W., Mikhailova N., Pitluck S., Pierson B.K., Blankenship R.E.
BMC Genomics 12:334-334(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29366 / DSM 635 / J-10-fl.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000909 Genomic DNA. Translation: ABY36300.1.
RefSeqYP_001636689.1. NC_010175.1.

3D structure databases

ProteinModelPortalA9WHH4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING324602.Caur_3101.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY36300; ABY36300; Caur_3101.
GeneID5827575.
KEGGcau:Caur_3101.
PATRIC21417531. VBIChlAur28763_3505.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000033410.
KOK03439.
OMARHKPWAN.
OrthoDBEOG6K6VBC.
ProtClustDBCLSK955837.

Enzyme and pathway databases

BioCycCAUR324602:GIXU-3150-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CHLAA
AccessionPrimary (citable) accession number: A9WHH4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: February 5, 2008
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways