A9WG69 (MURB_CHLAA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	Caur_0752

Organism

Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)

Taxonomic identifier

324602 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000332453
Regions
	Domain	22 – 195	174	FAD-binding PCMH-type
Sites
	Active site	169	1	By similarity
	Active site	223	1	Proton donor By similarity
	Active site	293	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9WG69 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 082D9876A590963C	FASTA	297	31,747
	10 20 30 40 50 60 MPVALPVTLY PDEPMARHSS WRAGGTARYY AEPATPDEAI ALAAWAREQQ LPLIWIGRGT 70 80 90 100 110 120 NLLVRDEGFD GVIASYRGQR WELIEHGETA EVWIEAGAPM AGTARRLAAM GWAGLEWAEG 130 140 150 160 170 180 LPGAVGGAIV GNAGCYGGSV AEVLITADLL LNGSECVEWS VHDLAYTYRE SVLKQLLHTG 190 200 210 220 230 240 IPPLVLAGRF RLQRGDPAAL TARMKAIAAE RKQKTPAGSS CGSVFKNPAG DFAGRLIEAA 250 260 270 280 290 GLKGVRIGDA EISTLHANYI INRGQARAAD ILALIDLART KVADQFGITL QLEVRII

References

[1]

"Complete sequence of Chloroflexus aurantiacus J-10-fl." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Pierson B.K., Blankenship R.E., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29366 / DSM 635 / J-10-fl.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000909 Genomic DNA. Translation: ABY33990.1.
RefSeq	YP_001634379.1. NC_010175.1.
3D structure databases
ProteinModelPortal	A9WG69.
ModBase	Search...
Protein-protein interaction databases
STRING	A9WG69.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5824836.
GenomeReviews	Gene locus Caur_0752 in contig CP000909_GR.
KEGG	cau:Caur_0752.
PATRIC	21412159. VBIChlAur28763_0857.
Phylogenomic databases
HOGENOM	HBG686573.
OMA	ISERHAN.
PhylomeDB	A9WG69.
ProtClustDB	PRK14649.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_CHLAA

Accession

Primary (citable) accession number: A9WG69

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program