Reviewed, UniProtKB/Swiss-Prot A9WDI1 (PYRD_CHLAA)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	Caur_3923

Organism

Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) [Complete proteome] [HAMAP]

Taxonomic identifier

324602 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O(2) = orotate + H(2)O(2).
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane proteinBy similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Dihydroorotate dehydrogenase		PRO_0000336462
Sites
	Active site	191	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9WDI1-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 8EA52C0ABAC594F0	FASTA	363	39,418
	10 20 30 40 50 60 MNRSEALFYR YGIRPILFRL GRGDAETAHE RTLHILALIS RSRLLCKTIG YLTTIRDQRL 70 80 90 100 110 120 QRTVCGIPFP NPVGLAAGMD KDGVAIPAWA ALGFGFVEVG TVTHHPQPGN PRPRLFRLPE 130 140 150 160 170 180 QEALINRMGF NNQGAASLAR RLARLQPAPI PVGVSIGKSK ITPLEQAIDD YRASFRQLFP 190 200 210 220 230 240 YAAYIAINVS SPNTPGLRQL QDADQLRALL AALQHDNAEL GRTDQRGPRP LLVKIAPDLS 250 260 270 280 290 300 DTAIEEVLTV CADHGVAGII ATNTTISREG LTGVDPRLAA EAGGLSGRPL IARALHVVRL 310 320 330 340 350 360 IARLTGNRLP IIGVGGIHTP DDGLRMLEAG ASLIQIYTGL VYYGPLLPRR INRAILTHSK VQQ

References

[1]

"Complete sequence of Chloroflexus aurantiacus J-10-fl." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Pierson B.K., Blankenship R.E., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000909 Genomic DNA. Translation: ABY37100.1.
RefSeq	YP_001637489.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5825733.
GenomeReviews	Gene locus Caur_3923 in contig CP000909_GR.
KEGG	cau:Caur_3923.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. DHO_DHase_1_2. IPR005719. DHO_DHase_2. IPR001295. Dihydroorotate_DHase_core. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_CHLAA

Accession

Primary (citable) accession number: A9WDI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	November 25, 2008
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)