A9WDH5 (A9WDH5_CHLAA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL ABY37094.1
EC=5.99.1.2 EMBL ABY37094.1

Gene names

Ordered Locus Names:

Caur_3917

Organism

Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) EMBL ABY37094.1

Taxonomic identifier

324602 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length	776 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS005733

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A9WDH5 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 2D83AA4C94391273	FASTA	776	86,765
	10 20 30 40 50 60 MGEKVVIVES PAKARTIQKY LGKGYKVASS MGHVRDLPKS GLAIDIEHDF APSYEIVKPK 70 80 90 100 110 120 VVSELSQALR NAEAVYLATD PDREGEAIAW HITQAVKLPK KTPVYRVVFQ EITRNAVQQA 130 140 150 160 170 180 LQQPRQINQN LVEAQQARRV LDRLVGYQLS PLLWDKVKRG LSAGRVQSVA VRLIVERERE 190 200 210 220 230 240 IEQFQPQEYW TIEADLLKDA GVAPRDLFRA VLIERDGKKL EKFSIENREQ AEAIVADLQG 250 260 270 280 290 300 AAYTVVRVTR RDKRRSPAPP FTTSTLQQEA ARKLGFSAKK TMTLAQRLYE GVDIGGEDGM 310 320 330 340 350 360 VGLITYMRTD SVQVAAEAQT EARDVIGKRF GKEYLPDQPP VYKTKAKGAQ EAHEAIRPTS 370 380 390 400 410 420 SARLPEQLSG KLENDLWRLY DLIWKRFIAS QMAPAVFDST TVDIAATPVV AGAPAYLFRA 430 440 450 460 470 480 TGSVLKFPGF LAVYNVSLDE GEEDEDSERR LPPLAEGEAL QLVELLPIQH FTEPPPRYTE 490 500 510 520 530 540 ASLVKELERL GIGRPSTYAS ILSTIQEREY VEMVDKKLIP TTLGRVVTDL LVEHFGNIVD 550 560 570 580 590 600 YDFTSALEQQ LDDIAEGSKK WVPVLREFYG PFRSTLEQAQ RQMRNVKREE IVTDLDCPKC 610 620 630 640 650 660 GQGKLVIRFG RNGEFLACSR YNREGDGESC DFTSDFHRDE QGQIVIDKAS APETSDVLCN 670 680 690 700 710 720 VCGRPMVIKK SRFGPFLGCS GYPECNNTRR IGRDGKPVPL PEPTGVQCPK CGEGELLRRR 730 740 750 760 770 GKFGRPFYGC SRYPKCDYIT NTLDEAQAGE APAEPAKNEP ALPSPSRSSK RTRKSA

References

[1]

"Complete sequence of Chloroflexus aurantiacus J-10-fl." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Pierson B.K., Blankenship R.E., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29366 / DSM 635 / J-10-fl.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000909 Genomic DNA. Translation: ABY37094.1.
RefSeq	YP_001637483.1. NC_010175.1.
3D structure databases
ProteinModelPortal	A9WDH5.
ModBase	Search...
Protein-protein interaction databases
STRING	A9WDH5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5825727.
GenomeReviews	Gene locus Caur_3917 in contig CP000909_GR.
KEGG	cau:Caur_3917.
PATRIC	21419322. VBIChlAur28763_4392.
Phylogenomic databases
HOGENOM	HBG512935.
OMA	EEYWSID.
PhylomeDB	A9WDH5.
ProtClustDB	PRK06599.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A9WDH5_CHLAA

Accession

Primary (citable) accession number: A9WDH5

Entry history

Integrated into UniProtKB/TrEMBL:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)