A9WCU2 (K6PF_CHLAA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase
Short name=Phosphofructokinase
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase

Gene names

Name:	pfkA
Ordered Locus Names:	Caur_0258

Organism

Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) [Reference proteome] [HAMAP]

Taxonomic identifier

324602 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chloroflexi › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus › Chloroflexus aurantiacus

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	6-phosphofructokinase HAMAP-Rule MF_00339		PRO_1000079308
Regions
	Nucleotide binding	25 – 29	5	ATP By similarity
	Nucleotide binding	167 – 171	5	ATP By similarity
	Nucleotide binding	184 – 200	17	ATP By similarity
Sites
	Active site	140	1	Proton acceptor By similarity
	Metal binding	198	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	200	1	Magnesium By similarity
	Binding site	175	1	Substrate By similarity
	Binding site	272	1	Substrate By similarity
	Binding site	278	1	Substrate By similarity
	Binding site	281	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9WCU2 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: C8B7B858A07B643C	FASTA	356	37,703
	10 20 30 40 50 60 MASKKQRIGV LTSGGDAPGL NAVIRAVVKS ASGLGWEVIG IHDGFEGLLG TKSYRVLTNA 70 80 90 100 110 120 DVQGLLPRGG TILRTTNKGH FGPRRSDELS EADPYVRAVK AIEEMGLRAL ITIGGEGTQR 130 140 150 160 170 180 IALELHKLGA PVIGVPKTID NDLAGTDRTF GFDTALQVAT DAIDRLHTTA ASHNRVMVLE 190 200 210 220 230 240 VMGRHTGWIA LHAGLAGGAD VILIPEIPFS IERVAEKVMA RDQQGSSFSI IVVAEGARPR 250 260 270 280 290 300 GGSEMYIAEG RLGGIGHWVG EQLEKLTAKE VRVVVLGHLQ RGGSPSPYDR LLSTRYGAAA 310 320 330 340 350 VQAAARGIYG EMVALRGQDI VTVPLAEACG HLNRVRPHSD LVLCARSLGI AFGDEL

References

[1]

"Complete sequence of Chloroflexus aurantiacus J-10-fl." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Pierson B.K., Blankenship R.E., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29366 / DSM 635 / J-10-fl.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000909 Genomic DNA. Translation: ABY33511.1.
RefSeq	YP_001633900.1. NC_010175.1.
3D structure databases
ProteinModelPortal	A9WCU2.
ModBase	Search...
Protein-protein interaction databases
STRING	324602.Caur_0258.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABY33511; ABY33511; Caur_0258.
GeneID	5824865.
KEGG	cau:Caur_0258.
PATRIC	21411026. VBIChlAur28763_0298.
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000248869.
KO	K00850.
OMA	TVPLERY.
ProtClustDB	CLSK983903.
Enzyme and pathway databases
BioCyc	CAUR324602:GIXU-261-MONOMER.
UniPathway	UPA00109; UER00182.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase.
InterPro	IPR012003. ATP_PFK_prok. IPR012829. PFK. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
TIGRFAMs	TIGR02483. PFK_mixed. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_CHLAA

Accession

Primary (citable) accession number: A9WCU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program