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A9WCU2

- PFKA_CHLAA

UniProt

A9WCU2 - PFKA_CHLAA

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei15 – 151ATP; via amide nitrogenUniRule annotation
    Metal bindingi116 – 1161Magnesium; catalyticUniRule annotation
    Sitei117 – 1171Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
    Active sitei140 – 1401Proton acceptorUniRule annotation
    Binding sitei175 – 1751Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei235 – 2351SubstrateUniRule annotation
    Binding sitei272 – 2721Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi78 – 792ATPUniRule annotation
    Nucleotide bindingi115 – 1184ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCAUR324602:GIXU-261-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:Caur_0258
    OrganismiChloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)
    Taxonomic identifieri324602 [NCBI]
    Taxonomic lineageiBacteriaChloroflexiChloroflexiaChloroflexalesChloroflexineaeChloroflexaceaeChloroflexus
    ProteomesiUP000002008: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 356356ATP-dependent 6-phosphofructokinasePRO_1000079308Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer or homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi324602.Caur_0258.

    Structurei

    3D structure databases

    ProteinModelPortaliA9WCU2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni138 – 1403Substrate bindingUniRule annotation
    Regioni182 – 1843Substrate bindingUniRule annotation
    Regioni278 – 2814Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000248869.
    KOiK00850.
    OMAiHRHRAHA.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_01976. Phosphofructokinase_III.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012829. PFK.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A9WCU2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASKKQRIGV LTSGGDAPGL NAVIRAVVKS ASGLGWEVIG IHDGFEGLLG    50
    TKSYRVLTNA DVQGLLPRGG TILRTTNKGH FGPRRSDELS EADPYVRAVK 100
    AIEEMGLRAL ITIGGEGTQR IALELHKLGA PVIGVPKTID NDLAGTDRTF 150
    GFDTALQVAT DAIDRLHTTA ASHNRVMVLE VMGRHTGWIA LHAGLAGGAD 200
    VILIPEIPFS IERVAEKVMA RDQQGSSFSI IVVAEGARPR GGSEMYIAEG 250
    RLGGIGHWVG EQLEKLTAKE VRVVVLGHLQ RGGSPSPYDR LLSTRYGAAA 300
    VQAAARGIYG EMVALRGQDI VTVPLAEACG HLNRVRPHSD LVLCARSLGI 350
    AFGDEL 356
    Length:356
    Mass (Da):37,703
    Last modified:February 5, 2008 - v1
    Checksum:iC8B7B858A07B643C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000909 Genomic DNA. Translation: ABY33511.1.
    RefSeqiYP_001633900.1. NC_010175.1.

    Genome annotation databases

    EnsemblBacteriaiABY33511; ABY33511; Caur_0258.
    GeneIDi5824865.
    KEGGicau:Caur_0258.
    PATRICi21411026. VBIChlAur28763_0298.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000909 Genomic DNA. Translation: ABY33511.1 .
    RefSeqi YP_001633900.1. NC_010175.1.

    3D structure databases

    ProteinModelPortali A9WCU2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 324602.Caur_0258.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABY33511 ; ABY33511 ; Caur_0258 .
    GeneIDi 5824865.
    KEGGi cau:Caur_0258.
    PATRICi 21411026. VBIChlAur28763_0298.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000248869.
    KOi K00850.
    OMAi HRHRAHA.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci CAUR324602:GIXU-261-MONOMER.

    Family and domain databases

    HAMAPi MF_01976. Phosphofructokinase_III.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012829. PFK.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02483. PFK_mixed. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29366 / DSM 635 / J-10-fl.

    Entry informationi

    Entry nameiPFKA_CHLAA
    AccessioniPrimary (citable) accession number: A9WCU2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3