Reviewed, UniProtKB/Swiss-Prot A9WBM7 (PROA_CHLAA)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Caur_1616

Organism

Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) [Complete proteome] [HAMAP]

Taxonomic identifier

324602 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Gamma-glutamyl phosphate reductase		PRO_0000340877

Sequences

	Sequence		Length	Mass (Da)
	A9WBM7-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 68BC84715CCE3378	FASTA	422	44,515
	10 20 30 40 50 60 MVDLEAIGRR AKTAARALAK LSTEQKNAAL CAIADGLLAR QDKILAANAA DVADAEKGGT 70 80 90 100 110 120 PPAIVDRMLL TPARLAAIAG DCRQVASLPD PVGEIFDRRE LPSGLRLYKR RVPIGVIGAI 130 140 150 160 170 180 YEARPNVTVD IASLCLKAGN AVILRGGSDI ARSVAATTEV IALALEQAGL PAFAVQSIID 190 200 210 220 230 240 PDRELVRQLL RLDRYVDMII PRGGAGLHRF CVENATVPVI VGGMGVSHIY VEPSADFARA 250 260 270 280 290 300 VPVIVNAKVQ RPGACNALDT LLVHRAAAST FLPLVAAALA QHGVELRCDL EALAILADAP 310 320 330 340 350 360 GHEAWNLKPA SPTDFGCEFL ALIVAIKIVG SIDEALDHIA LYGGHSEAIL TGDPISAERF 370 380 390 400 410 420 TREVDATAVF VNASTRFNDG GQFGLGAEVA ISTNRLHARG PMGLQELTTY TWIGEGDYLV RA

References

[1]

"Complete sequence of Chloroflexus aurantiacus J-10-fl." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Pierson B.K., Blankenship R.E., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000909 Genomic DNA. Translation: ABY34834.1.
RefSeq	YP_001635223.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5826062.
GenomeReviews	Gene locus Caur_1616 in contig CP000909_GR.
KEGG	cau:Caur_1616.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DHase_C. IPR016162. Ald_DHase_N. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DHase. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_CHLAA

Accession

Primary (citable) accession number: A9WBM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	February 5, 2008
Last modified:	November 25, 2008
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)