A9WAX3 (SYE_CHLAA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Caur_1535

Organism

Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)

Taxonomic identifier

324602 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_1000074317
Regions
	Motif	14 – 24	11	"HIGH" region HAMAP MF_00022_B
	Motif	261 – 265	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	264	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9WAX3 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 4666A8FA4194733F	FASTA	503	56,742
	10 20 30 40 50 60 MSSIEGPVRV RFAPSPTGSL HIGGVRTALF NWLCARHYGG QFILRIEDTD EKRFVPGAAD 70 80 90 100 110 120 DISASLRWVG IDWDEGPDVG GPYGPYVQSE RFEQGIYQPF INQLLEAGLA YMSFTTEEEL 130 140 150 160 170 180 AQMRAAAEAA GIKAFRFRGP ERDWPLERQR EEAASGKPYT IRLKTPLEGE TRFRDLIRGG 190 200 210 220 230 240 DEIVVQNNQL QDIVLIKSTG MPVYHFAHLV DDHLMKITHV MRGEEWVPST PYHVLLYDFF 250 260 270 280 290 300 GWPRPVFAHL PAILRQDGRG KLSKRKDDVA TQRFRERGYL PETIFNYLAL QGWSYDGVTE 310 320 330 340 350 360 IMTRDELIAR FTLERIQPSP ARWNPEKLRD MNGIYIRKLT TEQLAERVLP FMQRAGLIGD 370 380 390 400 410 420 PASETERAYL ISLIPLIHER LEELQEAPDL LAFFYQDVVP GETYNPADLI PKKHDAAQTV 430 440 450 460 470 480 ALLRAAHEAL SQQTDWTPPA LETTLRALCE QLQVKPGPLF GAIRVAITGR SVAPPLFDTL 490 500 AGVGRERSLS RLAAAIAALE NLA

References

[1]

"Complete sequence of Chloroflexus aurantiacus J-10-fl." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Pierson B.K., Blankenship R.E., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29366 / DSM 635 / J-10-fl.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000909 Genomic DNA. Translation: ABY34754.1.
RefSeq	YP_001635143.1. NC_010175.1.
3D structure databases
ProteinModelPortal	A9WAX3.
ModBase	Search...
Protein-protein interaction databases
STRING	A9WAX3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5828641.
GenomeReviews	Gene locus Caur_1535 in contig CP000909_GR.
KEGG	cau:Caur_1535.
PATRIC	21413957. VBIChlAur28763_1745.
Phylogenomic databases
HOGENOM	HBG628189.
OMA	MAHIPLI.
PhylomeDB	A9WAX3.
ProtClustDB	PRK01406.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_CHLAA

Accession

Primary (citable) accession number: A9WAX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program