Reviewed, UniProtKB/Swiss-Prot A9WA97 (SYN_CHLAA)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	Caur_1428

Organism

Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) [Complete proteome] [HAMAP]

Taxonomic identifier

324602 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm By similarity HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_1000081845

Sequences

	Sequence		Length	Mass (Da)
	A9WA97-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 9FA56F03C01D8690	FASTA	440	50,042
	10 20 30 40 50 60 MSLLPTATVA TIARYVGQRV TLAGWVYHKT EKGKLIFILL RDGSGTIQCV TFKKNVSEET 70 80 90 100 110 120 FATAQSLTQE SSCRITGSVR ADERAPGGYE LDVESIELIG PSHEYPITPK EHGVEFLMAH 130 140 150 160 170 180 RHLWVRSAKQ HAILRIRAEV IAAAQEWLNE QGFVRFDTPI LTATAAEGTT NLFATDYFDL 190 200 210 220 230 240 GKAYLAQTGQ LYVEAGMMAF GKVYCFGPTF RAEKSKTRRH LTEFWMIEPE VAFADHEDNM 250 260 270 280 290 300 RLQEEFVSAI VARVLERRRD DLQTLERDTT LLEQVRPPFP RITYDEAIEL IAAHQGEVEG 310 320 330 340 350 360 ADPLPWGEDF GAPHETLIAS KFDRPVFVER FPSAVKAFYM QPDPERPEVA LCADLLAPEG 370 380 390 400 410 420 YGEIIGGSQR IHDPILLEQR IREHGLRIED YEWYLDLRRY GTVPHSGFGM GIERVVAWIT 430 440 GTRHIRETIP FPRQLYRIYP

References

[1]

"Complete sequence of Chloroflexus aurantiacus J-10-fl." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Pierson B.K., Blankenship R.E., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000909 Genomic DNA. Translation: ABY34656.1.
RefSeq	YP_001635045.1.
3D structure databases
SMR	A9WA97. Positions 15-440.
ModBase	Search...
Genome annotation databases
GeneID	5828534.
GenomeReviews	Gene locus Caur_1428 in contig CP000909_GR.
KEGG	cau:Caur_1428.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG745843.
OMA	LQKKRHS.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_CHLAA

Accession

Primary (citable) accession number: A9WA97

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	February 9, 2010
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)