A9W982 (SYE2_METEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	Mext_4753

Organism

Methylobacterium extorquens (strain PA1) [Complete proteome] [HAMAP]

Taxonomic identifier

419610 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Glutamate--tRNA ligase 2 HAMAP MF_00022_B		PRO_0000367709
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00022_B
	Motif	242 – 246	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	245	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9W982 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 8224A7CD69B79AA8	FASTA	449	48,704
	10 20 30 40 50 60 MAASPLVRFA PSPTGFLHIG NARPALLNAL FARREGGRFL LRLDDTDRER STEEFATAAA 70 80 90 100 110 120 EDLGWLGIEP DLFFRQSDRT ALYDTAAERL KAAGRLYPCY ETPEELDRRR KRQLGRGLPP 130 140 150 160 170 180 IYDRAALSLT EAERAAFEAD GRQPHWRFKL DHRVVAWNDL VRGESHVDCA SLSDPVLVRA 190 200 210 220 230 240 DGSYLYTLPS VVDDAEVGVT HVIRGEDHVT NTGVQVQLFE ALAAAVPAFG HHNLLTTADG 250 260 270 280 290 300 EGLSKRLGHL SLRSLREAGY EPAAVRSLAV LTGSAEAVRP IASLDELAGL VDLAHLSRAP 310 320 330 340 350 360 ARFDPAELDG MNARLVHDMP LEAVHERLAA LGVPAEAAEA FWLAVRANLG RVSDAAAWWQ 370 380 390 400 410 420 VVNGPVTPVI AEPDFIARAA DLLPEAPFGP GTWKAWTDAV KAETGAKGRA LFMPLRLALT 430 440 GLDHGPDLAA LLPLIGRERA VRRLAGETA

References

[1]

"Complete sequence of Methylobacterium extorquens PA1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Marx C., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000908 Genomic DNA. Translation: ABY33121.1.
RefSeq	YP_001642192.1. NC_010172.1.
3D structure databases
ProteinModelPortal	A9W982.
ModBase	Search...
Protein-protein interaction databases
STRING	A9W982.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5834557.
GenomeReviews	Gene locus Mext_4753 in contig CP000908_GR.
KEGG	mex:Mext_4753.
PATRIC	22540215. VBIMetExt98426_4839.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	DQERIEW.
ProtClustDB	PRK12558.
Enzyme and pathway databases
BioCyc	MEXT419610:MEXT_4753-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_METEP

Accession

Primary (citable) accession number: A9W982

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program