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A9W8P8

- PNP_METEP

UniProt

A9W8P8 - PNP_METEP

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Protein

Polyribonucleotide nucleotidyltransferase

Gene
pnp, Mext_4027
Organism
Methylobacterium extorquens (strain PA1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi487 – 4871Magnesium By similarity
Metal bindingi493 – 4931Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciMEXT419610:GI32-4083-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:Mext_4027
OrganismiMethylobacterium extorquens (strain PA1)
Taxonomic identifieri419610 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000008546: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 745745Polyribonucleotide nucleotidyltransferaseUniRule annotationPRO_0000381907Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi419610.Mext_4027.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini554 – 61360KHAdd
BLAST
Domaini623 – 69169S1 motifAdd
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9W8P8-1 [UniParc]FASTAAdd to Basket

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MFDVQREELI WGDRKLVLET GKTARQADGS VVATYGETTV LATVVAGKEP    50
KAGIDFLPLT VNYQERAYAA GRIPGGYFKR EGRPSEKETL VSRLIDRPIR 100
PLFVEGWRND TQVVVTVLSH DLENDPDIVA MVAASAALTL SGVPFMGPIG 150
AARVGYLNGG YKLNPLVTEI AESTLDLVVA GTQDAVLMVE SEAKELSEDV 200
MLGAVMFGHK HFQPVIEAII RLAEKAAKEP RDFTPPENAD VEAAVLAVAE 250
TELREAYKKT VKQERYAAVD AVKAKVVAAL CPAEGEQKFS PEKVKAAFKE 300
AQSKVVRWNI LDTGSRIDGR DVKTVRSIVS EVGVLPRAHG SSLFTRGETQ 350
ALVVATLGTG EDEQFIDALE GTYKERFLLH YNFPPYSVGE TGRMGSPGRR 400
EIGHGKLAWR AIRPVLPPAH EFPYTIRVVS EITESNGSSS MASVCGGSLS 450
LMDAGVPLRR PVAGIAMGLI LEGERFAVLS DILGDEDHLG DMDFKVAGSE 500
EGITSLQMDI KIAGITEEIM KIALAQAKDG RAHILGEMSK ALTAARPELG 550
EYAPRIETMQ IPTDKIRDVI GTGGKIIREI VEKTGAKINI EDTGIVKIAS 600
SDGKAIKAAY NWIRSIVAEP EAGTIYDGTI VKIMEFGAFV NFFGAKDGLV 650
HISELAAQRV AKVGDVVKEG QKVKVKFLGA DERGKIRLSM KVVDQETGED 700
LTEKLKAERA ERGEPEREER SDRGDRGDRG PRRDRGERRR ESSGE 745
Length:745
Mass (Da):81,002
Last modified:September 1, 2009 - v2
Checksum:iA1F2D7EBD2D871DD
GO

Sequence cautioni

The sequence ABY32397.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000908 Genomic DNA. Translation: ABY32397.1. Different initiation.
RefSeqiYP_001641468.1. NC_010172.1.

Genome annotation databases

EnsemblBacteriaiABY32397; ABY32397; Mext_4027.
GeneIDi5833629.
KEGGimex:Mext_4027.
PATRICi22538691. VBIMetExt98426_4084.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000908 Genomic DNA. Translation: ABY32397.1 . Different initiation.
RefSeqi YP_001641468.1. NC_010172.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 419610.Mext_4027.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABY32397 ; ABY32397 ; Mext_4027 .
GeneIDi 5833629.
KEGGi mex:Mext_4027.
PATRICi 22538691. VBIMetExt98426_4084.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci MEXT419610:GI32-4083-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PA1.

Entry informationi

Entry nameiPNP_METEP
AccessioniPrimary (citable) accession number: A9W8P8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 1, 2009
Last modified: May 14, 2014
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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