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A9W8P8

- PNP_METEP

UniProt

A9W8P8 - PNP_METEP

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Methylobacterium extorquens (strain PA1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 2 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi487 – 4871MagnesiumUniRule annotation
    Metal bindingi493 – 4931MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciMEXT419610:GI32-4083-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:Mext_4027
    OrganismiMethylobacterium extorquens (strain PA1)
    Taxonomic identifieri419610 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
    ProteomesiUP000008546: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 745745Polyribonucleotide nucleotidyltransferasePRO_0000381907Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi419610.Mext_4027.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini554 – 61360KHUniRule annotationAdd
    BLAST
    Domaini623 – 69169S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218326.
    KOiK00962.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A9W8P8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFDVQREELI WGDRKLVLET GKTARQADGS VVATYGETTV LATVVAGKEP    50
    KAGIDFLPLT VNYQERAYAA GRIPGGYFKR EGRPSEKETL VSRLIDRPIR 100
    PLFVEGWRND TQVVVTVLSH DLENDPDIVA MVAASAALTL SGVPFMGPIG 150
    AARVGYLNGG YKLNPLVTEI AESTLDLVVA GTQDAVLMVE SEAKELSEDV 200
    MLGAVMFGHK HFQPVIEAII RLAEKAAKEP RDFTPPENAD VEAAVLAVAE 250
    TELREAYKKT VKQERYAAVD AVKAKVVAAL CPAEGEQKFS PEKVKAAFKE 300
    AQSKVVRWNI LDTGSRIDGR DVKTVRSIVS EVGVLPRAHG SSLFTRGETQ 350
    ALVVATLGTG EDEQFIDALE GTYKERFLLH YNFPPYSVGE TGRMGSPGRR 400
    EIGHGKLAWR AIRPVLPPAH EFPYTIRVVS EITESNGSSS MASVCGGSLS 450
    LMDAGVPLRR PVAGIAMGLI LEGERFAVLS DILGDEDHLG DMDFKVAGSE 500
    EGITSLQMDI KIAGITEEIM KIALAQAKDG RAHILGEMSK ALTAARPELG 550
    EYAPRIETMQ IPTDKIRDVI GTGGKIIREI VEKTGAKINI EDTGIVKIAS 600
    SDGKAIKAAY NWIRSIVAEP EAGTIYDGTI VKIMEFGAFV NFFGAKDGLV 650
    HISELAAQRV AKVGDVVKEG QKVKVKFLGA DERGKIRLSM KVVDQETGED 700
    LTEKLKAERA ERGEPEREER SDRGDRGDRG PRRDRGERRR ESSGE 745
    Length:745
    Mass (Da):81,002
    Last modified:September 1, 2009 - v2
    Checksum:iA1F2D7EBD2D871DD
    GO

    Sequence cautioni

    The sequence ABY32397.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000908 Genomic DNA. Translation: ABY32397.1. Different initiation.
    RefSeqiYP_001641468.1. NC_010172.1.

    Genome annotation databases

    EnsemblBacteriaiABY32397; ABY32397; Mext_4027.
    GeneIDi5833629.
    KEGGimex:Mext_4027.
    PATRICi22538691. VBIMetExt98426_4084.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000908 Genomic DNA. Translation: ABY32397.1 . Different initiation.
    RefSeqi YP_001641468.1. NC_010172.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 419610.Mext_4027.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABY32397 ; ABY32397 ; Mext_4027 .
    GeneIDi 5833629.
    KEGGi mex:Mext_4027.
    PATRICi 22538691. VBIMetExt98426_4084.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218326.
    KOi K00962.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci MEXT419610:GI32-4083-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PA1.

    Entry informationi

    Entry nameiPNP_METEP
    AccessioniPrimary (citable) accession number: A9W8P8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 49 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3