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A9W8P8

- PNP_METEP

UniProt

A9W8P8 - PNP_METEP

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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Methylobacterium extorquens (strain PA1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi487 – 4871MagnesiumUniRule annotation
Metal bindingi493 – 4931MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciMEXT419610:GI32-4083-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Mext_4027
OrganismiMethylobacterium extorquens (strain PA1)
Taxonomic identifieri419610 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000008546: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 745745Polyribonucleotide nucleotidyltransferasePRO_0000381907Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi419610.Mext_4027.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini554 – 61360KHUniRule annotationAdd
BLAST
Domaini623 – 69169S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9W8P8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFDVQREELI WGDRKLVLET GKTARQADGS VVATYGETTV LATVVAGKEP
60 70 80 90 100
KAGIDFLPLT VNYQERAYAA GRIPGGYFKR EGRPSEKETL VSRLIDRPIR
110 120 130 140 150
PLFVEGWRND TQVVVTVLSH DLENDPDIVA MVAASAALTL SGVPFMGPIG
160 170 180 190 200
AARVGYLNGG YKLNPLVTEI AESTLDLVVA GTQDAVLMVE SEAKELSEDV
210 220 230 240 250
MLGAVMFGHK HFQPVIEAII RLAEKAAKEP RDFTPPENAD VEAAVLAVAE
260 270 280 290 300
TELREAYKKT VKQERYAAVD AVKAKVVAAL CPAEGEQKFS PEKVKAAFKE
310 320 330 340 350
AQSKVVRWNI LDTGSRIDGR DVKTVRSIVS EVGVLPRAHG SSLFTRGETQ
360 370 380 390 400
ALVVATLGTG EDEQFIDALE GTYKERFLLH YNFPPYSVGE TGRMGSPGRR
410 420 430 440 450
EIGHGKLAWR AIRPVLPPAH EFPYTIRVVS EITESNGSSS MASVCGGSLS
460 470 480 490 500
LMDAGVPLRR PVAGIAMGLI LEGERFAVLS DILGDEDHLG DMDFKVAGSE
510 520 530 540 550
EGITSLQMDI KIAGITEEIM KIALAQAKDG RAHILGEMSK ALTAARPELG
560 570 580 590 600
EYAPRIETMQ IPTDKIRDVI GTGGKIIREI VEKTGAKINI EDTGIVKIAS
610 620 630 640 650
SDGKAIKAAY NWIRSIVAEP EAGTIYDGTI VKIMEFGAFV NFFGAKDGLV
660 670 680 690 700
HISELAAQRV AKVGDVVKEG QKVKVKFLGA DERGKIRLSM KVVDQETGED
710 720 730 740
LTEKLKAERA ERGEPEREER SDRGDRGDRG PRRDRGERRR ESSGE
Length:745
Mass (Da):81,002
Last modified:September 1, 2009 - v2
Checksum:iA1F2D7EBD2D871DD
GO

Sequence cautioni

The sequence ABY32397.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000908 Genomic DNA. Translation: ABY32397.1. Different initiation.
RefSeqiYP_001641468.1. NC_010172.1.

Genome annotation databases

EnsemblBacteriaiABY32397; ABY32397; Mext_4027.
GeneIDi5833629.
KEGGimex:Mext_4027.
PATRICi22538691. VBIMetExt98426_4084.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000908 Genomic DNA. Translation: ABY32397.1 . Different initiation.
RefSeqi YP_001641468.1. NC_010172.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 419610.Mext_4027.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABY32397 ; ABY32397 ; Mext_4027 .
GeneIDi 5833629.
KEGGi mex:Mext_4027.
PATRICi 22538691. VBIMetExt98426_4084.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci MEXT419610:GI32-4083-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PA1.

Entry informationi

Entry nameiPNP_METEP
AccessioniPrimary (citable) accession number: A9W8P8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 1, 2009
Last modified: October 29, 2014
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3