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A9W8M8

- BIOB_METEP

UniProt

A9W8M8 - BIOB_METEP

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Protein
Biotin synthase
Gene
bioB, Mext_4006
Organism
Methylobacterium extorquens (strain PA1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi110 – 1101Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi142 – 1421Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi202 – 2021Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi274 – 2741Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMEXT419610:GI32-4062-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Mext_4006
OrganismiMethylobacterium extorquens (strain PA1)
Taxonomic identifieri419610 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000008546: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Biotin synthaseUniRule annotation
PRO_0000381460Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi419610.Mext_4006.

Structurei

3D structure databases

ProteinModelPortaliA9W8M8.
SMRiA9W8M8. Positions 16-325.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A9W8M8-1 [UniParc]FASTAAdd to Basket

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MSDTVVSLTA SPAAIRHDWT LAEIQAIHDM PLLDLVHRAG VVHRAHNDPA    50
DIQRAALLSI KTGGCPEDCA YCPQSAHHKG ANLPRERLMP VDAVLKEAAA 100
AKANGAHRFC MGAAWRKPKD GPDFDAVLEM VRGVRGLGME ACVTLGMLTQ 150
SQAQRLAEAG LTSYNHNLDT GPEFYGDIIS TRTYDDRLQT LEHVRQAGIG 200
VCCGGIVGMG ERVRDRAEML LVLANHAPHP ESVPINALVA VEGTPLEDRP 250
PIDPLDLVRM CATARIVMPK ARVRLSAGRK SLTREAQILC FLAGANSIFY 300
GERLLTTANN EADADAELLR DIGVPVPEVT LAAAE 335
Length:335
Mass (Da):36,063
Last modified:February 5, 2008 - v1
Checksum:i1D3EB9757274E706
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000908 Genomic DNA. Translation: ABY32377.1.
RefSeqiYP_001641448.1. NC_010172.1.

Genome annotation databases

EnsemblBacteriaiABY32377; ABY32377; Mext_4006.
GeneIDi5831487.
KEGGimex:Mext_4006.
PATRICi22538647. VBIMetExt98426_4062.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000908 Genomic DNA. Translation: ABY32377.1 .
RefSeqi YP_001641448.1. NC_010172.1.

3D structure databases

ProteinModelPortali A9W8M8.
SMRi A9W8M8. Positions 16-325.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 419610.Mext_4006.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABY32377 ; ABY32377 ; Mext_4006 .
GeneIDi 5831487.
KEGGi mex:Mext_4006.
PATRICi 22538647. VBIMetExt98426_4062.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MEXT419610:GI32-4062-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PA1.

Entry informationi

Entry nameiBIOB_METEP
AccessioniPrimary (citable) accession number: A9W8M8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 5, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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