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A9W8M8 (BIOB_METEP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Mext_4006
OrganismMethylobacterium extorquens (strain PA1) [Complete proteome] [HAMAP]
Taxonomic identifier419610 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 335335Biotin synthase HAMAP-Rule MF_01694
PRO_0000381460

Sites

Metal binding651Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding721Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1101Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1421Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2021Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2741Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A9W8M8 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: 1D3EB9757274E706

FASTA33536,063
        10         20         30         40         50         60 
MSDTVVSLTA SPAAIRHDWT LAEIQAIHDM PLLDLVHRAG VVHRAHNDPA DIQRAALLSI 

        70         80         90        100        110        120 
KTGGCPEDCA YCPQSAHHKG ANLPRERLMP VDAVLKEAAA AKANGAHRFC MGAAWRKPKD 

       130        140        150        160        170        180 
GPDFDAVLEM VRGVRGLGME ACVTLGMLTQ SQAQRLAEAG LTSYNHNLDT GPEFYGDIIS 

       190        200        210        220        230        240 
TRTYDDRLQT LEHVRQAGIG VCCGGIVGMG ERVRDRAEML LVLANHAPHP ESVPINALVA 

       250        260        270        280        290        300 
VEGTPLEDRP PIDPLDLVRM CATARIVMPK ARVRLSAGRK SLTREAQILC FLAGANSIFY 

       310        320        330 
GERLLTTANN EADADAELLR DIGVPVPEVT LAAAE 

« Hide

References

[1]"Complete sequence of Methylobacterium extorquens PA1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., Marx C., Richardson P.
Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PA1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000908 Genomic DNA. Translation: ABY32377.1.
RefSeqYP_001641448.1. NC_010172.1.

3D structure databases

ProteinModelPortalA9W8M8.
SMRA9W8M8. Positions 16-325.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING419610.Mext_4006.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY32377; ABY32377; Mext_4006.
GeneID5831487.
KEGGmex:Mext_4006.
PATRIC22538647. VBIMetExt98426_4062.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2331944.

Enzyme and pathway databases

BioCycMEXT419610:GI32-4062-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_METEP
AccessionPrimary (citable) accession number: A9W8M8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 5, 2008
Last modified: February 19, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways