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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Methylobacterium extorquens (strain PA1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei78Charge relay systemUniRule annotation1
Active sitei158Charge relay systemUniRule annotation1
Active sitei182Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMEXT419610:G1GAN-3426-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:Mext_3323
OrganismiMethylobacterium extorquens (strain PA1)
Taxonomic identifieri419610 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000008546 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000951471 – 493Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST493

Proteomic databases

PRIDEiA9W748

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi419610.Mext_3323

Structurei

3D structure databases

SMRiA9W748
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P Bacteria
COG0154 LUCA
HOGENOMiHOG000116699
KOiK02433
OMAiPITQNVE
OrthoDBiPOG091H00O9

Family and domain databases

Gene3Di3.90.1300.10, 1 hit
HAMAPiMF_00120 GatA, 1 hit
InterProiView protein in InterPro
IPR000120 Amidase
IPR020556 Amidase_CS
IPR023631 Amidase_dom
IPR036928 AS_sf
IPR004412 GatA
PANTHERiPTHR11895 PTHR11895, 1 hit
PfamiView protein in Pfam
PF01425 Amidase, 1 hit
SUPFAMiSSF75304 SSF75304, 1 hit
TIGRFAMsiTIGR00132 gatA, 1 hit
PROSITEiView protein in PROSITE
PS00571 AMIDASES, 1 hit

Sequencei

Sequence statusi: Complete.

A9W748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTALNELTLA EARDGLKAKD FSAREIAQAH LDAIDRAKAL NAYIVATPDR
60 70 80 90 100
ALKMAEVSDQ KIAKGEARPL EGLPLGIKDL FATQGVDTTA GSKILEGFEP
110 120 130 140 150
HYESNVSSQL WRDGAVMLGK LNLDEFAMGS SNETSAYGKT ISPWRRQGSY
160 170 180 190 200
APLVPGGSSG GSAAAVAAHL CLGATATDTG GSIRQPAAFT GTVGIKPTYG
210 220 230 240 250
RCSRWGIIAY ASSLDQAGPI ARTVQDCAIL LGSMAGHDPR DTTSVDMPVP
260 270 280 290 300
DFEAAISRGV KGLTIGIPKE YRVEGMPAEI QRLWDQGADW LREAGATIKE
310 320 330 340 350
ISLPHTQYAL PAYYIVAPAE ASSNLARYDG VRYGLRVPGK DIAGMYENTR
360 370 380 390 400
AAGFGREVKR RIMIGTYVLS AGYYDAYYVR AQKIRTLIKR DFEAAYASGV
410 420 430 440 450
DAILTPATPS AAFGIGEMAS ADPVEMYLND VFTVTVNMAG LPGISVPAGL
460 470 480 490
DAQGLPLGLQ LIGRPFDEET LFAAAQTIEN AAGRISLPKA WWA
Length:493
Mass (Da):52,433
Last modified:February 5, 2008 - v1
Checksum:i232CDF65940D77AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000908 Genomic DNA Translation: ABY31710.1
RefSeqiWP_012254588.1, NC_010172.1

Genome annotation databases

EnsemblBacteriaiABY31710; ABY31710; Mext_3323
KEGGimex:Mext_3323

Similar proteinsi

Entry informationi

Entry nameiGATA_METEP
AccessioniPrimary (citable) accession number: A9W748
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: February 5, 2008
Last modified: May 23, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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