A9W733 (A9W733_METEP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

Mext_3308

Organism

Methylobacterium extorquens (strain PA1) [Complete proteome] [HAMAP]

Taxonomic identifier

419610 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	910 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733 RuleBase RU000518
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A9W733 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 0BE4FDB8856FDE36	FASTA	910	98,716
	10 20 30 40 50 60 MKVVVVESPA KAKTINKYLG RDYEVLASFG HIRDLPAKDG SVDPEADFAM IWEVEDRGAK 70 80 90 100 110 120 RVSEIARAVK GAEKLILATD PDREGEAISW HVIEALTARK ALKGIPVERV TFNAITKASV 130 140 150 160 170 180 DAAMKKPRQI DQALVDAYLA RRALDYLVGF NLSPVLWRKL PGARSAGRVQ SVALRLVVER 190 200 210 220 230 240 EMEIERFKPR EYWSIIATLV TEAGGVFEAR LVGADGKRIQ RLDVGNAEEA AAFKRDLELA 250 260 270 280 290 300 TFQVASIEAK PAKRHPQPPF TTSTLQQEAS RKLGMAPAQT MRVAQRLYEG VDVGGETVGI 310 320 330 340 350 360 ITYMRTDGVD MAPEAIQDAR QVIGREFGDA YVPGAPRKYT VKAKNAQEAH EAVRPTDMGR 370 380 390 400 410 420 LPKHVARHLE PEQAKLYDLI WTRTIASQME SAELERTTVE VSANVGPRRI DLRATGQVVK 430 440 450 460 470 480 FDGFLALYQE GKDDEEDEDA KRLPAMKAGD ALKRERIAST QHFTEPPPRY SEASLVKRME 490 500 510 520 530 540 ELGIGRPSTY AAVLQTLRDR EYVRIEKKRL QPEDKGRLVT GFLESFFKRY VEYDFTASLE 550 560 570 580 590 600 EQLDRVSNAE IDWRSVLRDF WRDFSAAIGG TKELRVAEVL EALNGLLGPH IFPEKADGGD 610 620 630 640 650 660 PRACPTCGAG QLSLKLGKFG AFIGCSNYPE CKYTRQLSAS GVEGEGDGSS AEGGQPGVRV 670 680 690 700 710 720 LGEDPATGLP VTLRDGRFGP FVQLGEASAE KGAEKPKRSS LPKGMTPGSV TLEIALRLLS 730 740 750 760 770 780 LPREVAKHPE TGEPILANLG RFGPYVQHGK TYANLGKDDD VLEIGANRAI DLIVAKEQGG 790 800 810 820 830 840 GRGRPSSDPG RTLGQHPDGA ALVVKAGKYG PYVSDGSVNA TLPKTMAAES LTLEQAIDLV 850 860 870 880 890 900 NAKRASGGGK KPVRKTASRA RAPAKKAEAG EAKKPAVRKT AAKTAAKAPA TKAAAKPAAA 910 KKATAKTKAG

References

[1]

"Complete sequence of Methylobacterium extorquens PA1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Marx C., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000908 Genomic DNA. Translation: ABY31695.1.
RefSeq	YP_001640766.1. NC_010172.1.
3D structure databases
ProteinModelPortal	A9W733.
ModBase	Search...
Protein-protein interaction databases
STRING	A9W733.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5833571.
GenomeReviews	Gene locus Mext_3308 in contig CP000908_GR.
KEGG	mex:Mext_3308.
PATRIC	22537216. VBIMetExt98426_3355.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	KKVMPRL.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A9W733_METEP

Accession

Primary (citable) accession number: A9W733

Entry history

Integrated into UniProtKB/TrEMBL:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)